Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Nitrosococcus; Nitrosococcus oceani

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2995 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3JEM2|Q3JEM2_NITOC Thiosulfate sulfurtransferase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=Noc_0191 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.34NLARR6 pKa = 11.84HH7 pKa = 5.75CFIFLGLLLVPTLGAAGLNIFPEE30 pKa = 4.68LDD32 pKa = 3.58SNDD35 pKa = 3.85NNNTSSNNTSQEE47 pKa = 3.88STQNFSNTADD57 pKa = 4.2PEE59 pKa = 4.4PEE61 pKa = 4.31ADD63 pKa = 3.79TNASPPANKK72 pKa = 10.22DD73 pKa = 3.3EE74 pKa = 5.32IIAEE78 pKa = 4.39TKK80 pKa = 10.32AGCATDD86 pKa = 3.84PASCGVTLSSFLDD99 pKa = 3.35STGFGEE105 pKa = 4.77TEE107 pKa = 4.25PNNHH111 pKa = 6.15AMGADD116 pKa = 3.0AMEE119 pKa = 4.75FGVEE123 pKa = 4.03YY124 pKa = 10.53AGQLYY129 pKa = 10.53SPEE132 pKa = 4.09DD133 pKa = 3.71VDD135 pKa = 3.39WFRR138 pKa = 11.84ITTTEE143 pKa = 4.09SNQMLTVNFNVPGLNDD159 pKa = 3.07ITGWNLSIRR168 pKa = 11.84DD169 pKa = 3.44SGGNIFSEE177 pKa = 5.26VYY179 pKa = 9.59TGFDD183 pKa = 4.06FGPEE187 pKa = 4.11SPLQTILSRR196 pKa = 11.84AGTYY200 pKa = 9.52YY201 pKa = 11.08VVVKK205 pKa = 10.11SLKK208 pKa = 9.24QAQEE212 pKa = 3.97EE213 pKa = 4.6TRR215 pKa = 11.84SSDD218 pKa = 3.35QSGEE222 pKa = 3.58ADD224 pKa = 4.93LIYY227 pKa = 10.73EE228 pKa = 4.52HH229 pKa = 7.05LPHH232 pKa = 7.41EE233 pKa = 4.39YY234 pKa = 10.23RR235 pKa = 11.84LAAFLGDD242 pKa = 3.66SQVTTEE248 pKa = 4.23PLDD251 pKa = 3.66VNFFDD256 pKa = 6.29AEE258 pKa = 4.26VEE260 pKa = 4.36PNDD263 pKa = 4.81SRR265 pKa = 11.84DD266 pKa = 3.38EE267 pKa = 4.41ANPLTFATPLASNVTMEE284 pKa = 4.44GLISGPLIFGSVGFAFEE301 pKa = 4.49EE302 pKa = 4.33DD303 pKa = 3.1WFVYY307 pKa = 8.93DD308 pKa = 3.52TAGNEE313 pKa = 3.7ILSIEE318 pKa = 4.22FCASQDD324 pKa = 4.1CEE326 pKa = 4.15DD327 pKa = 4.1STWQVTVYY335 pKa = 10.97DD336 pKa = 3.97EE337 pKa = 4.32NEE339 pKa = 3.88RR340 pKa = 11.84MLLTGRR346 pKa = 11.84TDD348 pKa = 3.31MEE350 pKa = 3.93QNYY353 pKa = 9.95YY354 pKa = 10.72LGIRR358 pKa = 11.84NPGKK362 pKa = 8.64YY363 pKa = 10.13FIRR366 pKa = 11.84IGVAPALDD374 pKa = 4.12EE375 pKa = 4.42EE376 pKa = 4.91SGGAQYY382 pKa = 11.08VCSIDD387 pKa = 3.15PTMPLKK393 pKa = 10.69DD394 pKa = 3.83CPSPSEE400 pKa = 3.79RR401 pKa = 11.84TLLVEE406 pKa = 5.02SPWHH410 pKa = 5.52QYY412 pKa = 10.38NFTVTSTKK420 pKa = 10.41LPPLMSEE427 pKa = 3.99VDD429 pKa = 3.57NPP431 pKa = 3.65

Molecular weight: 47.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3J6M5|Q3J6M5_NITOC ATP synthase subunit a OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=atpB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.02NLKK11 pKa = 9.99RR12 pKa = 11.84KK13 pKa = 7.29RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TQGGRR28 pKa = 11.84AIINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2995

0

2995

987868

37

1867

329.8

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.483 ± 0.051

0.996 ± 0.017

4.707 ± 0.031

6.604 ± 0.041

3.945 ± 0.03

7.61 ± 0.039

2.419 ± 0.022

5.859 ± 0.03

4.182 ± 0.038

11.286 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.165 ± 0.021

3.183 ± 0.023

4.961 ± 0.036

4.472 ± 0.033

6.583 ± 0.041

5.649 ± 0.034

4.969 ± 0.031

6.573 ± 0.035

1.484 ± 0.022

2.87 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski