Legionella oakridgensis ATCC 33761 = DSM 21215
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2907 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0BHA3|W0BHA3_9GAMM Coenzyme F420-reducing hydrogenase alpha subunit OS=Legionella oakridgensis ATCC 33761 = DSM 21215 OX=1268635 GN=Loa_02480 PE=3 SV=1
MM1 pKa = 7.49 SNSDD5 pKa = 3.62 FEE7 pKa = 4.55 QFLTALGAFEE17 pKa = 5.21 SGIDD21 pKa = 3.5 TTQTQTTSWMQYY33 pKa = 10.18 LQVFDD38 pKa = 4.77 PNRR41 pKa = 11.84 GNVDD45 pKa = 3.65 PNSVNLSDD53 pKa = 4.62 PNDD56 pKa = 3.79 LAEE59 pKa = 4.5 LQYY62 pKa = 10.72 HH63 pKa = 5.06 VHH65 pKa = 5.66 NTLGFLGKK73 pKa = 10.09 YY74 pKa = 8.49 QFGEE78 pKa = 4.07 PLLIDD83 pKa = 4.07 LGYY86 pKa = 10.69 YY87 pKa = 10.18 SPAPTGYY94 pKa = 10.76 YY95 pKa = 8.46 GTTATNEE102 pKa = 4.0 WQGTWTGKK110 pKa = 10.41 NGVDD114 pKa = 3.44 SKK116 pKa = 11.6 EE117 pKa = 3.82 EE118 pKa = 3.77 FMTNVQEE125 pKa = 4.04 LAIRR129 pKa = 11.84 EE130 pKa = 4.21 AFAMNMGVINQYY142 pKa = 10.96 LNQAGKK148 pKa = 8.9 TIDD151 pKa = 4.36 DD152 pKa = 4.01 FLGHH156 pKa = 6.76 EE157 pKa = 4.32 FTYY160 pKa = 10.69 TRR162 pKa = 11.84 FGEE165 pKa = 3.99 AHH167 pKa = 6.79 IATVTMSGILASAHH181 pKa = 5.41 LQGPGGVAQLLLHH194 pKa = 6.72 NIASSDD200 pKa = 3.6 EE201 pKa = 4.18 YY202 pKa = 10.41 GTNILFYY209 pKa = 8.68 MDD211 pKa = 4.15 KK212 pKa = 10.59 FGGYY216 pKa = 7.47 HH217 pKa = 5.8 TPFGTNGDD225 pKa = 4.06 DD226 pKa = 3.82 FLVGSDD232 pKa = 3.73 YY233 pKa = 11.6 SEE235 pKa = 4.01 TFMGEE240 pKa = 4.0 GGHH243 pKa = 6.31 NEE245 pKa = 4.09 YY246 pKa = 9.04 EE247 pKa = 4.3 TGGGNDD253 pKa = 4.6 KK254 pKa = 10.79 IIIAQNITGNDD265 pKa = 3.52 VIQDD269 pKa = 3.11 FDD271 pKa = 3.33 IARR274 pKa = 11.84 DD275 pKa = 3.92 VISLTKK281 pKa = 10.54 FPGLTFADD289 pKa = 4.48 LSITALNNNAVIHH302 pKa = 6.16 FPNGQTVTLNNVDD315 pKa = 3.62 ASQISANQFVSGPYY329 pKa = 9.97 KK330 pKa = 10.64 IMWNAGSGDD339 pKa = 3.8 TIIEE343 pKa = 4.21 NFNLQHH349 pKa = 7.28 DD350 pKa = 5.44 LIDD353 pKa = 4.08 LNYY356 pKa = 10.93 AFASNNLALYY366 pKa = 9.05 EE367 pKa = 4.31 EE368 pKa = 4.87 NGSAVIDD375 pKa = 4.03 VIGNNQRR382 pKa = 11.84 IILAGIEE389 pKa = 4.2 LDD391 pKa = 3.71 EE392 pKa = 4.89 LSPFHH397 pKa = 7.16 FIKK400 pKa = 10.8 APTDD404 pKa = 3.7 FAQVHH409 pKa = 5.96 FGINSDD415 pKa = 3.45 HH416 pKa = 6.7 TPPVVDD422 pKa = 4.76 PGDD425 pKa = 3.98 ANTGNDD431 pKa = 3.26 NPADD435 pKa = 3.9 GGDD438 pKa = 3.6 NNNQQGTMDD447 pKa = 4.32 GDD449 pKa = 3.68 VFAYY453 pKa = 7.38 TWNWGARR460 pKa = 11.84 DD461 pKa = 3.9 VIQGFDD467 pKa = 3.28 ATEE470 pKa = 4.07 DD471 pKa = 4.06 KK472 pKa = 10.8 IDD474 pKa = 4.21 LQNFWTSFDD483 pKa = 4.26 RR484 pKa = 11.84 IQFYY488 pKa = 11.47 DD489 pKa = 3.59 NANGDD494 pKa = 3.88 AVIDD498 pKa = 3.82 LTTLNNQTITLADD511 pKa = 3.42 VSVQQLSSANFIGVTGTMGIHH532 pKa = 6.24 NEE534 pKa = 4.16 PNNPPANEE542 pKa = 4.11 PTDD545 pKa = 3.83 PSPPSEE551 pKa = 4.16 NDD553 pKa = 3.53 AIPPEE558 pKa = 4.48 DD559 pKa = 3.59 TTGSGDD565 pKa = 3.53 SYY567 pKa = 11.21 VFTWKK572 pKa = 9.52 WGAAEE577 pKa = 4.37 TINNFDD583 pKa = 3.31 VGQDD587 pKa = 3.74 VIDD590 pKa = 5.08 LKK592 pKa = 11.12 SFWSSYY598 pKa = 7.87 SQFSIYY604 pKa = 10.73 DD605 pKa = 3.63 NPQGNAVIDD614 pKa = 4.13 LSQLNNQTITIQGVSSAEE632 pKa = 3.73 LSAKK636 pKa = 10.47 NIVGVAGTIDD646 pKa = 3.31 QALTGHH652 pKa = 6.99 SIPPSDD658 pKa = 3.96 HH659 pKa = 6.96 TDD661 pKa = 3.52 PPDD664 pKa = 3.38 TGTVTDD670 pKa = 4.91 VISHH674 pKa = 6.87 PVNSTNLVASDD685 pKa = 3.76 AVQDD689 pKa = 3.72 VFNFTWSWGSQNVIDD704 pKa = 5.15 GFNPTQDD711 pKa = 3.38 TVDD714 pKa = 4.27 LSRR717 pKa = 11.84 FWTTNDD723 pKa = 3.72 QVDD726 pKa = 4.25 IYY728 pKa = 11.79 NNAQGDD734 pKa = 4.09 AVIDD738 pKa = 3.99 LSALNNQTITLLGVNADD755 pKa = 5.46 DD756 pKa = 5.2 LNQDD760 pKa = 3.08 NVIFF764 pKa = 4.45
Molecular weight: 83.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.745
IPC_protein 3.795
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.923
Patrickios 1.507
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|W0BDB8|W0BDB8_9GAMM Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase OS=Legionella oakridgensis ATCC 33761 = DSM 21215 OX=1268635 GN=Loa_01076 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.18 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 DD15 pKa = 3.36 HH16 pKa = 6.66 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 AGRR28 pKa = 11.84 LVLKK32 pKa = 10.36 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2907
0
2907
808281
29
3405
278.0
31.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.324 ± 0.048
1.236 ± 0.016
4.694 ± 0.033
5.863 ± 0.048
4.374 ± 0.033
6.178 ± 0.048
2.846 ± 0.022
7.237 ± 0.043
5.634 ± 0.04
11.051 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.621 ± 0.023
4.263 ± 0.037
4.15 ± 0.026
4.787 ± 0.033
4.727 ± 0.036
6.15 ± 0.038
5.21 ± 0.033
6.058 ± 0.042
1.176 ± 0.016
3.421 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here