Burkholderia plantarii
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6432 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B6RZU9|A0A0B6RZU9_BURPL Chaperone SurA OS=Burkholderia plantarii OX=41899 GN=surA PE=3 SV=1
MM1 pKa = 7.78 SYY3 pKa = 7.45 TTPSSLSDD11 pKa = 3.07 FMGRR15 pKa = 11.84 FEE17 pKa = 5.28 NGGLLSGNTSGGTTTFYY34 pKa = 11.33 YY35 pKa = 10.53 EE36 pKa = 4.4 PSTNTINFGYY46 pKa = 10.4 GINLSFASGSTQARR60 pKa = 11.84 GVIDD64 pKa = 3.81 SALSAANISLSDD76 pKa = 3.96 ALWSKK81 pKa = 10.51 IKK83 pKa = 10.66 NATAATRR90 pKa = 11.84 GALVSDD96 pKa = 4.58 LNNLVTNDD104 pKa = 3.47 GSSPDD109 pKa = 3.56 WQAVAASVTSAYY121 pKa = 7.59 TQQIVVPEE129 pKa = 4.32 LQSHH133 pKa = 6.36 IPNLASLPATTQWALEE149 pKa = 4.02 DD150 pKa = 3.61 AFYY153 pKa = 11.19 NSPSLLGPLVYY164 pKa = 10.31 GAANSGNLASIAVQLAFNEE183 pKa = 4.33 EE184 pKa = 4.29 TPNAAQSGAEE194 pKa = 3.98 LRR196 pKa = 11.84 NLARR200 pKa = 11.84 AALALGGSITFAPGGNTISSIDD222 pKa = 3.4 WSKK225 pKa = 11.94 ADD227 pKa = 3.55 IASVGQFLSQIVSSAPVGKK246 pKa = 10.57 GGSTADD252 pKa = 3.19 AYY254 pKa = 9.67 VNKK257 pKa = 9.2 WGSSASASYY266 pKa = 8.76 LTLTDD271 pKa = 3.2 SLYY274 pKa = 11.04 SYY276 pKa = 11.24 VEE278 pKa = 3.8 SQGRR282 pKa = 11.84 YY283 pKa = 7.99 VVDD286 pKa = 3.61 TGQATYY292 pKa = 11.21 ASIVSQNSDD301 pKa = 3.55 DD302 pKa = 4.26 PNFGNLTAADD312 pKa = 3.49 IAALNGMSVSATLTAGQVIDD332 pKa = 4.03 IPTSVTEE339 pKa = 4.27 GLPASLSSSLSYY351 pKa = 10.53 TLDD354 pKa = 3.2 VTTGRR359 pKa = 11.84 VVASPITDD367 pKa = 3.07 GMFGGSVTVQNSATGAFLGYY387 pKa = 9.78 FDD389 pKa = 5.51 AGTVTGEE396 pKa = 3.54 AAAASGQWTLSLNGGRR412 pKa = 11.84 SVTFNADD419 pKa = 2.9 SSISVSDD426 pKa = 3.8 PAGSGSNFAPGASINVSSTGSATVLSSPGGTFSPVFVPTGNAYY469 pKa = 8.22 GTDD472 pKa = 3.38 LSTINSGQDD481 pKa = 2.7 VGLATDD487 pKa = 3.96 ISALMGIAAPIAIDD501 pKa = 4.87 AVALDD506 pKa = 4.02 YY507 pKa = 11.41 LSDD510 pKa = 4.99 RR511 pKa = 11.84 IQYY514 pKa = 11.17 SNVSDD519 pKa = 3.1 KK520 pKa = 10.48 WVAAFIGPAPEE531 pKa = 4.44 GTFSLSSTLLSSTFDD546 pKa = 3.37 PTTAALIEE554 pKa = 4.39 LKK556 pKa = 9.46 NTIANRR562 pKa = 11.84 PKK564 pKa = 8.84 PTWVSISIDD573 pKa = 3.29 VTGFNSVPKK582 pKa = 9.23 TSASVGILQQTDD594 pKa = 3.34 AQVSPGTSLVQATMAGSQEE613 pKa = 3.83 AAQVLNAYY621 pKa = 8.97 INKK624 pKa = 9.68 GYY626 pKa = 10.42 AAAGAQTATSIATTLSGEE644 pKa = 4.3 PTDD647 pKa = 4.12 FTSPIAKK654 pKa = 8.76 IQSWYY659 pKa = 8.3 GTTRR663 pKa = 11.84 IQGAGNGSTIGPTAGVPDD681 pKa = 5.24 EE682 pKa = 3.98 ISAYY686 pKa = 10.45 GSTAVAGAVPSSNGGYY702 pKa = 9.76 LIHH705 pKa = 6.4 STAPADD711 pKa = 3.83 TVRR714 pKa = 11.84 ALQDD718 pKa = 3.44 IQSLTPTAVAVARR731 pKa = 11.84 SVAGGATAFGAAEE744 pKa = 4.42 FAAVQADD751 pKa = 3.72 SDD753 pKa = 3.94 GSAASEE759 pKa = 3.67 ATARR763 pKa = 11.84 NLAVFANQAWGSALTEE779 pKa = 3.97 YY780 pKa = 10.82 LQVAEE785 pKa = 4.8 NVSAMSQEE793 pKa = 3.96 LSNVSAEE800 pKa = 4.17 LTALEE805 pKa = 4.16 PVSYY809 pKa = 10.13 EE810 pKa = 3.74 VTGNLVNGYY819 pKa = 9.67 SYY821 pKa = 11.31 YY822 pKa = 10.91 SSGDD826 pKa = 3.19 AAFAADD832 pKa = 4.27 TFNGFADD839 pKa = 3.84 GMKK842 pKa = 9.81 TFAATKK848 pKa = 10.26 DD849 pKa = 3.71 GLDD852 pKa = 3.41 RR853 pKa = 11.84 ALLAIAQADD862 pKa = 3.59 NASRR866 pKa = 11.84 IFVGEE871 pKa = 4.15 NGSSTTLEE879 pKa = 3.94 SDD881 pKa = 2.8 TDD883 pKa = 3.55 IFVAGGGNEE892 pKa = 4.07 TVADD896 pKa = 3.99 GTGQDD901 pKa = 4.08 SILVSSGSGNLYY913 pKa = 10.0 ISNFKK918 pKa = 10.86 AGINGDD924 pKa = 3.47 QLQFLGVGDD933 pKa = 4.11 NVVASDD939 pKa = 4.41 DD940 pKa = 4.3 GNGGTLLKK948 pKa = 10.6 YY949 pKa = 10.81 GSGNFIDD956 pKa = 5.64 LIGVSAGSLNLYY968 pKa = 10.06 QNITGVSAISFVGVSSPASFGLGGNRR994 pKa = 11.84 IYY996 pKa = 10.87 DD997 pKa = 3.9 GQVHH1001 pKa = 6.55 ISSITGTNSGSTLSGDD1017 pKa = 3.75 SAATTLTGGAGNDD1030 pKa = 3.49 TFKK1033 pKa = 11.41 VEE1035 pKa = 4.02 GNGYY1039 pKa = 9.35 VINGAGGTNTVSYY1052 pKa = 10.82 GDD1054 pKa = 3.56 VPFGVSVNLQTGTDD1068 pKa = 3.58 NLGTTLFAIQNVEE1081 pKa = 3.69 GSSYY1085 pKa = 11.44 DD1086 pKa = 3.45 DD1087 pKa = 4.39 TIAGDD1092 pKa = 3.73 TNNNVLDD1099 pKa = 4.32 GKK1101 pKa = 10.71 GGNDD1105 pKa = 3.44 TLIGGGGNDD1114 pKa = 3.52 TYY1116 pKa = 11.68 VFDD1119 pKa = 4.73 AGYY1122 pKa = 11.0 GSVTIEE1128 pKa = 4.75 NGLAPNGIPSSEE1140 pKa = 4.14 LLFGAGIDD1148 pKa = 5.37 ANDD1151 pKa = 3.1 LWFTRR1156 pKa = 11.84 SGNDD1160 pKa = 2.98 LTIQVLGTDD1169 pKa = 3.28 NQVTVKK1175 pKa = 10.25 GWFSNAWQQLASISLSDD1192 pKa = 3.88 GLQVSPQTVATILKK1206 pKa = 9.51 LQALYY1211 pKa = 10.72 VAANPGFDD1219 pKa = 4.49 PDD1221 pKa = 3.94 TAQAMPGGIDD1231 pKa = 3.23 LTSYY1235 pKa = 10.21 FGPVSNPTTVPTATNVALATEE1256 pKa = 4.28 HH1257 pKa = 7.03 LYY1259 pKa = 10.61 TSGVATQGANLAGSAATQINNPVGSAWSANVYY1291 pKa = 10.75 ANNLAYY1297 pKa = 10.08 GSKK1300 pKa = 10.48 ALPAPYY1306 pKa = 10.37 GSGEE1310 pKa = 4.04 NYY1312 pKa = 10.02 AYY1314 pKa = 10.22 QYY1316 pKa = 10.94 YY1317 pKa = 8.24 VTGSSQPYY1325 pKa = 7.73 VALSVRR1331 pKa = 11.84 PWGQYY1336 pKa = 10.96 APGTSISTTPPGVTSYY1352 pKa = 10.07 QQIYY1356 pKa = 10.45 ALTPSTFYY1364 pKa = 11.41 VKK1366 pKa = 10.04 ATTSVSPSSSSNPDD1380 pKa = 2.84 SSLVFASDD1388 pKa = 3.59 PNVMVNAINAVTEE1401 pKa = 4.17 AVDD1404 pKa = 4.03 LATTGATLAQAISTADD1420 pKa = 3.55 TARR1423 pKa = 11.84 QTALGSAVAANSAGAAFGSQPAVQARR1449 pKa = 11.84 ADD1451 pKa = 3.58 ATTAEE1456 pKa = 4.44 ADD1458 pKa = 3.58 LAAALAQWAPVQSNLVTAGGVLASSQAALNGILPGTQVSTQQRR1501 pKa = 11.84 TGYY1504 pKa = 9.63 GLSGPSTVTVTYY1516 pKa = 6.11 TTNYY1520 pKa = 10.25 SFYY1523 pKa = 10.18 TSTDD1527 pKa = 3.1 QAEE1530 pKa = 4.29 FNALQSAVSNAQGAVGEE1547 pKa = 4.62 ADD1549 pKa = 3.83 FLMLSTLQSALQDD1562 pKa = 3.63 FGAYY1566 pKa = 9.49 EE1567 pKa = 4.09 RR1568 pKa = 11.84 VQVGGTSSNLGADD1581 pKa = 3.73 DD1582 pKa = 5.68 GGDD1585 pKa = 3.71 LLIAAGGGYY1594 pKa = 8.09 HH1595 pKa = 5.48 TVVGGAGRR1603 pKa = 11.84 DD1604 pKa = 3.46 TFVFGGWNDD1613 pKa = 3.44 TSSATVQNFATGTQGDD1629 pKa = 4.32 RR1630 pKa = 11.84 LLLIPAQNRR1639 pKa = 11.84 TVYY1642 pKa = 9.44 LTDD1645 pKa = 3.78 GGGTTDD1651 pKa = 4.29 ASFFVDD1657 pKa = 3.2 NGGVDD1662 pKa = 3.91 TVALAGVSLGALSLYY1677 pKa = 11.14 DD1678 pKa = 3.63 NFNGVDD1684 pKa = 3.23 TASFANEE1691 pKa = 3.82 SHH1693 pKa = 6.19 GVSIDD1698 pKa = 3.47 LATVTPRR1705 pKa = 11.84 SYY1707 pKa = 11.23 DD1708 pKa = 3.03 GSTHH1712 pKa = 5.3 IRR1714 pKa = 11.84 NLVGSNYY1721 pKa = 10.32 GDD1723 pKa = 3.62 TLTGDD1728 pKa = 3.58 EE1729 pKa = 4.42 QDD1731 pKa = 3.56 NVITGGAGNDD1741 pKa = 3.73 VLSGGAGNNTLDD1753 pKa = 4.25 GGGGNNTVSYY1763 pKa = 9.86 AGSPGGVTVDD1773 pKa = 3.86 LTAGTASNGYY1783 pKa = 10.0 GGTDD1787 pKa = 3.32 TLSHH1791 pKa = 5.92 IQNVIGSGGNDD1802 pKa = 3.19 TMFGDD1807 pKa = 4.28 ANNNVLDD1814 pKa = 4.34 GNGGNDD1820 pKa = 3.32 VLVGGGGSDD1829 pKa = 3.31 TYY1831 pKa = 11.63 VFDD1834 pKa = 4.48 AGYY1837 pKa = 9.48 GQDD1840 pKa = 3.54 VIVNGVAGNAGPSGQLTLGAGLSAANLRR1868 pKa = 11.84 FVQSGNDD1875 pKa = 3.41 LVIEE1879 pKa = 4.13 VLGTTEE1885 pKa = 4.03 QVTVQDD1891 pKa = 3.84 WFQAGASYY1899 pKa = 10.56 RR1900 pKa = 11.84 QLATIALADD1909 pKa = 3.84 GTTLSMTDD1917 pKa = 3.21 VNGLVAEE1924 pKa = 4.35 GHH1926 pKa = 6.69 DD1927 pKa = 4.18 WTPLSTPSASTSPTLAGRR1945 pKa = 11.84 YY1946 pKa = 8.82 VYY1948 pKa = 10.02 IRR1950 pKa = 11.84 KK1951 pKa = 9.3 NDD1953 pKa = 3.65 GASTGLVTSEE1963 pKa = 3.64 IQVWGTDD1970 pKa = 3.5 GSDD1973 pKa = 3.22 LALGKK1978 pKa = 10.29 AVTSSPSWGDD1988 pKa = 2.85 GTTAVNAVNGSTSASGGIFASASADD2013 pKa = 2.79 GWVEE2017 pKa = 3.59 VDD2019 pKa = 3.89 LGSVQTIAGVDD2030 pKa = 2.94 VWGRR2034 pKa = 11.84 DD2035 pKa = 3.62 DD2036 pKa = 5.56 GSEE2039 pKa = 4.05 EE2040 pKa = 3.9 MNGNYY2045 pKa = 8.4 TVYY2048 pKa = 10.74 VSNQDD2053 pKa = 3.16 MGGLDD2058 pKa = 3.73 AGQLQAVPGARR2069 pKa = 11.84 AYY2071 pKa = 10.35 QEE2073 pKa = 4.16 NQPGPMDD2080 pKa = 3.56 VKK2082 pKa = 9.52 VTSWQAPSTVTQTTGRR2098 pKa = 11.84 YY2099 pKa = 8.82 VYY2101 pKa = 10.43 VRR2103 pKa = 11.84 KK2104 pKa = 10.0 NDD2106 pKa = 3.65 GASTGLVTSEE2116 pKa = 3.74 IQVWGAGGVDD2126 pKa = 3.66 LALGKK2131 pKa = 8.44 TVTSSSSWSSDD2142 pKa = 2.62 TTAANAINGVTSASGGLFASASLDD2166 pKa = 3.03 GWIEE2170 pKa = 3.67 IDD2172 pKa = 3.81 LGSVQAIDD2180 pKa = 5.94 GIDD2183 pKa = 2.9 VWGRR2187 pKa = 11.84 DD2188 pKa = 3.36 DD2189 pKa = 5.73 GAFEE2193 pKa = 4.27 MNGDD2197 pKa = 3.53 YY2198 pKa = 10.24 TVYY2201 pKa = 10.79 VSNQDD2206 pKa = 3.73 LAGLDD2211 pKa = 3.4 ASQIQASVPNVQRR2224 pKa = 11.84 FQQNDD2229 pKa = 3.34 RR2230 pKa = 11.84 GPNDD2234 pKa = 3.38 VAVSSWQAPTALAATAGRR2252 pKa = 11.84 YY2253 pKa = 9.12 VYY2255 pKa = 10.45 VRR2257 pKa = 11.84 KK2258 pKa = 10.0 NDD2260 pKa = 3.65 GASTGLVTSEE2270 pKa = 3.8 IQVWGADD2277 pKa = 3.61 GSDD2280 pKa = 3.23 LALGKK2285 pKa = 8.41 TVTSSTSWGDD2295 pKa = 3.15 STMAANAVNGSTGASGGIFASASADD2320 pKa = 2.83 GWIEE2324 pKa = 3.73 VDD2326 pKa = 3.64 LGSVQTINGIDD2337 pKa = 3.0 VWGRR2341 pKa = 11.84 DD2342 pKa = 4.31 DD2343 pKa = 5.46 GALDD2347 pKa = 3.39 MNGNYY2352 pKa = 8.38 TVYY2355 pKa = 10.8 VSNQDD2360 pKa = 3.75 LAGLSAAQAQATAGVKK2376 pKa = 9.66 QFQQSQQSQQGALDD2390 pKa = 3.71 TAVTSWQTPATAATTLGRR2408 pKa = 11.84 YY2409 pKa = 9.26 VYY2411 pKa = 10.43 VRR2413 pKa = 11.84 KK2414 pKa = 10.0 NDD2416 pKa = 3.65 GASTGLVTSEE2426 pKa = 3.64 IQVWGTDD2433 pKa = 3.5 GSDD2436 pKa = 3.11 LALGKK2441 pKa = 8.45 TVTSSKK2447 pKa = 10.5 SWGDD2451 pKa = 3.19 STTAANAINGVTSASGGIFASASADD2476 pKa = 2.82 GWVEE2480 pKa = 3.42 IDD2482 pKa = 4.41 LGSAQTIAGIDD2493 pKa = 3.04 VWGRR2497 pKa = 11.84 DD2498 pKa = 4.31 DD2499 pKa = 5.46 GALDD2503 pKa = 3.39 MNGNYY2508 pKa = 8.89 TVFVSNQDD2516 pKa = 3.46 MAGLDD2521 pKa = 3.56 ANQIQSISGAKK2532 pKa = 9.94 AFQQNQQGPLDD2543 pKa = 3.75 VSVTSWQSAAGSMVQAMAAFSSSSAGATNTTFVPQTQQSSMLAASMQQ2590 pKa = 3.38
Molecular weight: 261.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.668
IPC2_protein 3.732
IPC_protein 3.808
Toseland 3.567
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.465
Solomon 3.808
Lehninger 3.77
Nozaki 3.91
DTASelect 4.215
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.923
Patrickios 1.316
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A0B6RS76|A0A0B6RS76_BURPL Uncharacterized protein OS=Burkholderia plantarii OX=41899 GN=BGL_2c01060 PE=4 SV=1
MM1 pKa = 7.59 SFRR4 pKa = 11.84 LTSRR8 pKa = 11.84 LHH10 pKa = 5.28 RR11 pKa = 11.84 AARR14 pKa = 11.84 RR15 pKa = 11.84 LSQLEE20 pKa = 3.55 PRR22 pKa = 11.84 RR23 pKa = 11.84 IVRR26 pKa = 11.84 TLRR29 pKa = 11.84 HH30 pKa = 4.88 HH31 pKa = 5.53 QARR34 pKa = 11.84 TRR36 pKa = 11.84 ALATRR41 pKa = 11.84 AVASTPRR48 pKa = 11.84 LDD50 pKa = 4.77 GLRR53 pKa = 11.84 AWLHH57 pKa = 5.75 SFLAAMRR64 pKa = 11.84 AHH66 pKa = 6.93 AGARR70 pKa = 11.84 RR71 pKa = 11.84 LGLPALLRR79 pKa = 11.84 KK80 pKa = 9.51 PSSLRR85 pKa = 11.84 LLALRR90 pKa = 11.84 RR91 pKa = 11.84 ANGPRR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 11.84 VNRR101 pKa = 11.84 PRR103 pKa = 11.84 RR104 pKa = 11.84 LSASPGWFAFGARR117 pKa = 3.97
Molecular weight: 13.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.506
IPC2_protein 11.14
IPC_protein 12.764
Toseland 12.925
ProMoST 13.422
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.442
Grimsley 12.969
Solomon 13.422
Lehninger 13.32
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.149
IPC_peptide 13.422
IPC2_peptide 12.413
IPC2.peptide.svr19 9.186
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6432
0
6432
2225259
44
7858
346.0
37.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.097 ± 0.058
0.89 ± 0.011
5.595 ± 0.027
4.877 ± 0.035
3.529 ± 0.019
8.575 ± 0.039
2.341 ± 0.016
4.305 ± 0.024
2.426 ± 0.029
10.345 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.129 ± 0.016
2.472 ± 0.024
5.472 ± 0.029
3.321 ± 0.02
7.718 ± 0.044
5.393 ± 0.059
5.308 ± 0.037
7.516 ± 0.024
1.36 ± 0.012
2.333 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here