Bovine leukemia virus (BLV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Deltaretrovirus

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O92812|O92812_BLV Gag-Pro-Pol polyprotein OS=Bovine leukemia virus OX=11901 GN=gag-pro-pol PE=4 SV=2
MM1 pKa = 7.49ASVVGWGPHH10 pKa = 5.48SLHH13 pKa = 6.78ACPALVLSNDD23 pKa = 3.22VTIDD27 pKa = 3.51AWCPLCGPHH36 pKa = 6.85EE37 pKa = 4.18RR38 pKa = 11.84LQFEE42 pKa = 5.11RR43 pKa = 11.84IDD45 pKa = 3.57TTLTCEE51 pKa = 3.95THH53 pKa = 7.04RR54 pKa = 11.84ITWTADD60 pKa = 2.77GRR62 pKa = 11.84PFGLNGTLFPRR73 pKa = 11.84LHH75 pKa = 7.17VSEE78 pKa = 4.88TRR80 pKa = 11.84PQGPRR85 pKa = 11.84RR86 pKa = 11.84LWINCPLPAVRR97 pKa = 11.84AQPGPVSLSPFEE109 pKa = 4.06QSPFQPYY116 pKa = 9.18QCQLPSASSDD126 pKa = 3.15GCPIIGHH133 pKa = 6.78GLLPWNNLVTHH144 pKa = 6.54PVLGKK149 pKa = 10.28VLILNQMANFSLLPPFDD166 pKa = 4.03TLLVDD171 pKa = 4.04PLRR174 pKa = 11.84LSVFAPDD181 pKa = 2.93TRR183 pKa = 11.84GAIRR187 pKa = 11.84YY188 pKa = 8.31LSTLLTLCPVTCILPLGEE206 pKa = 4.33PFSPNVPICRR216 pKa = 11.84FPRR219 pKa = 11.84DD220 pKa = 3.37TSEE223 pKa = 4.35PPLSEE228 pKa = 4.47FEE230 pKa = 4.65LPLIQTPGLSWSVPAIDD247 pKa = 5.57LFLTGPPSPYY257 pKa = 10.44DD258 pKa = 3.54RR259 pKa = 11.84LHH261 pKa = 5.97VWSSPQALQRR271 pKa = 11.84FLHH274 pKa = 6.92DD275 pKa = 3.76PTLTWSEE282 pKa = 3.97LVASGKK288 pKa = 10.09LRR290 pKa = 11.84LDD292 pKa = 3.72SPLKK296 pKa = 10.66LQLLEE301 pKa = 4.42NEE303 pKa = 4.0WLSRR307 pKa = 11.84LFF309 pKa = 3.92

Molecular weight: 34.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q77YG2|Q77YG2_BLV Env polyprotein OS=Bovine leukemia virus OX=11901 GN=env PE=4 SV=1
MM1 pKa = 7.34PKK3 pKa = 9.71EE4 pKa = 3.67RR5 pKa = 11.84RR6 pKa = 11.84SRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84PQPIIRR16 pKa = 11.84WQVLLVGGPTLYY28 pKa = 10.24MPARR32 pKa = 11.84PWFCPMMSPSMPGAPSAGPMNDD54 pKa = 3.53SNSKK58 pKa = 10.42GSTPRR63 pKa = 11.84SPARR67 pKa = 11.84PTVSPGPPMDD77 pKa = 5.52DD78 pKa = 3.81LLASMEE84 pKa = 4.35HH85 pKa = 6.83CSLDD89 pKa = 3.7CMSPRR94 pKa = 11.84PAPKK98 pKa = 10.57GPDD101 pKa = 3.56DD102 pKa = 4.58SGSTAPFRR110 pKa = 11.84PFALSPARR118 pKa = 11.84FHH120 pKa = 6.85FPPSSSPPSSPTNANCPRR138 pKa = 11.84PLATVAPSSGTAFFPGTTT156 pKa = 3.63

Molecular weight: 16.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3362

156

1417

560.3

61.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.852 ± 0.555

2.052 ± 0.326

3.867 ± 0.113

3.688 ± 0.336

2.855 ± 0.396

5.711 ± 0.346

2.439 ± 0.244

4.7 ± 0.35

3.48 ± 0.508

11.957 ± 0.918

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.952 ± 0.347

4.164 ± 0.255

12.255 ± 0.983

6.246 ± 0.503

5.473 ± 0.287

7.466 ± 0.546

5.413 ± 0.149

4.521 ± 0.197

2.677 ± 0.206

2.231 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski