Leptonychotes weddellii papillomavirus 5
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8B2T9|A0A2I8B2T9_9PAPI Replication protein E1 OS=Leptonychotes weddellii papillomavirus 5 OX=2077306 GN=E1 PE=3 SV=1
MM1 pKa = 7.75 APRR4 pKa = 11.84 AARR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.63 RR10 pKa = 11.84 ADD12 pKa = 3.1 AGSLYY17 pKa = 9.95 RR18 pKa = 11.84 HH19 pKa = 6.46 CVQGGDD25 pKa = 4.55 CIPDD29 pKa = 3.28 VVNKK33 pKa = 10.18 FEE35 pKa = 5.09 HH36 pKa = 6.18 KK37 pKa = 9.55 TPADD41 pKa = 4.87 RR42 pKa = 11.84 ILQIGGSLVYY52 pKa = 10.22 FGGAGISTGRR62 pKa = 11.84 GAGGYY67 pKa = 9.33 RR68 pKa = 11.84 PLGGTRR74 pKa = 11.84 TFTFGSRR81 pKa = 11.84 GAAGPGRR88 pKa = 11.84 LPPLLADD95 pKa = 3.41 TLGPVDD101 pKa = 3.36 ATVYY105 pKa = 10.64 EE106 pKa = 5.24 GITFDD111 pKa = 3.8 SPAVSVGVDD120 pKa = 3.32 EE121 pKa = 5.44 GPAIEE126 pKa = 4.48 LTPIGGRR133 pKa = 11.84 DD134 pKa = 3.72 GQPGSGAPTISTTTDD149 pKa = 2.48 SDD151 pKa = 4.06 VAILEE156 pKa = 4.58 VGTSTPSSRR165 pKa = 11.84 PTSRR169 pKa = 11.84 GGNVVSRR176 pKa = 11.84 SQFEE180 pKa = 4.18 NPTFVPTQPGTPGVGEE196 pKa = 4.74 FSAQEE201 pKa = 4.18 NILVDD206 pKa = 3.62 HH207 pKa = 6.86 SGGGVSVGEE216 pKa = 4.14 FEE218 pKa = 4.93 EE219 pKa = 5.75 IEE221 pKa = 4.32 LGDD224 pKa = 3.77 LGEE227 pKa = 4.39 RR228 pKa = 11.84 TDD230 pKa = 3.53 TGVPKK235 pKa = 10.49 TSTPKK240 pKa = 10.42 SRR242 pKa = 11.84 LGDD245 pKa = 3.59 LVSKK249 pKa = 10.89 AKK251 pKa = 10.23 SLYY254 pKa = 9.23 NRR256 pKa = 11.84 RR257 pKa = 11.84 VTQTRR262 pKa = 11.84 VEE264 pKa = 4.07 NPEE267 pKa = 3.67 FLGRR271 pKa = 11.84 PSSLVEE277 pKa = 4.24 FGFEE281 pKa = 3.87 NPAFEE286 pKa = 5.31 GDD288 pKa = 3.43 VTLTFDD294 pKa = 4.18 RR295 pKa = 11.84 VQEE298 pKa = 4.06 AKK300 pKa = 10.36 AAPDD304 pKa = 3.3 LAFRR308 pKa = 11.84 DD309 pKa = 4.03 IIKK312 pKa = 10.36 LSRR315 pKa = 11.84 PIYY318 pKa = 10.35 SEE320 pKa = 4.21 APGGRR325 pKa = 11.84 VRR327 pKa = 11.84 VSRR330 pKa = 11.84 LGTKK334 pKa = 8.53 GTIRR338 pKa = 11.84 LRR340 pKa = 11.84 SGTLIGGQTHH350 pKa = 6.1 YY351 pKa = 10.82 FRR353 pKa = 11.84 DD354 pKa = 3.84 LSSIGAPSLEE364 pKa = 4.03 MSVLGQTSGEE374 pKa = 4.31 STVVLGSDD382 pKa = 3.28 SSIINGVGLEE392 pKa = 4.16 GTVAFPEE399 pKa = 4.22 EE400 pKa = 3.95 QLLLDD405 pKa = 4.01 EE406 pKa = 5.47 LSEE409 pKa = 4.23 NFQNSQLVFSDD420 pKa = 3.16 SHH422 pKa = 6.4 QSHH425 pKa = 7.19 IIEE428 pKa = 4.55 LPRR431 pKa = 11.84 IAEE434 pKa = 4.41 SPRR437 pKa = 11.84 SSYY440 pKa = 11.04 VIYY443 pKa = 8.8 DD444 pKa = 3.8 TADD447 pKa = 3.37 STVVSHH453 pKa = 7.18 KK454 pKa = 10.86 GNEE457 pKa = 3.95 NYY459 pKa = 10.28 FISTLTPPDD468 pKa = 4.05 TEE470 pKa = 5.23 PDD472 pKa = 3.91 TEE474 pKa = 4.26 PQTPDD479 pKa = 3.01 EE480 pKa = 4.47 TPRR483 pKa = 11.84 APRR486 pKa = 11.84 RR487 pKa = 11.84 PLDD490 pKa = 4.3 DD491 pKa = 4.25 SDD493 pKa = 5.1 GLTFDD498 pKa = 4.33 LHH500 pKa = 6.56 PGLLRR505 pKa = 11.84 RR506 pKa = 11.84 KK507 pKa = 8.63 KK508 pKa = 10.4 KK509 pKa = 9.51 RR510 pKa = 11.84 VRR512 pKa = 11.84 YY513 pKa = 9.54 YY514 pKa = 10.22 IEE516 pKa = 3.8
Molecular weight: 55.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.184
IPC2_protein 5.181
IPC_protein 5.143
Toseland 5.105
ProMoST 5.232
Dawson 5.143
Bjellqvist 5.283
Wikipedia 5.016
Rodwell 5.067
Grimsley 5.041
Solomon 5.143
Lehninger 5.105
Nozaki 5.27
DTASelect 5.436
Thurlkill 5.105
EMBOSS 5.08
Sillero 5.347
Patrickios 4.368
IPC_peptide 5.156
IPC2_peptide 5.347
IPC2.peptide.svr19 5.305
Protein with the highest isoelectric point:
>tr|A0A2I8B2Q3|A0A2I8B2Q3_9PAPI Protein E7 OS=Leptonychotes weddellii papillomavirus 5 OX=2077306 GN=E7 PE=3 SV=1
MM1 pKa = 7.89 EE2 pKa = 5.27 KK3 pKa = 10.31 PQTLRR8 pKa = 11.84 ALAASLGEE16 pKa = 3.86 PLQRR20 pKa = 11.84 ILVQCVFCGAPMTWPDD36 pKa = 3.41 KK37 pKa = 10.98 VAFEE41 pKa = 4.32 NKK43 pKa = 9.13 CLSVVWCRR51 pKa = 11.84 EE52 pKa = 3.82 RR53 pKa = 11.84 FFGACVSCTNVRR65 pKa = 11.84 SYY67 pKa = 11.33 WDD69 pKa = 3.3 VLNNRR74 pKa = 11.84 GPTLEE79 pKa = 4.04 ATGVEE84 pKa = 4.36 SLFNKK89 pKa = 9.41 PLSDD93 pKa = 3.24 IPVRR97 pKa = 11.84 CMYY100 pKa = 10.58 CLAQLTVLEE109 pKa = 5.16 KK110 pKa = 10.34 IACAVNEE117 pKa = 4.2 RR118 pKa = 11.84 PFVLVRR124 pKa = 11.84 KK125 pKa = 9.44 LFRR128 pKa = 11.84 NTCSEE133 pKa = 3.98 CTQYY137 pKa = 11.38 DD138 pKa = 3.55 RR139 pKa = 11.84 SS140 pKa = 3.74
Molecular weight: 15.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.445
IPC2_protein 7.454
IPC_protein 7.293
Toseland 6.634
ProMoST 7.849
Dawson 7.98
Bjellqvist 8.521
Wikipedia 7.878
Rodwell 7.995
Grimsley 6.547
Solomon 8.039
Lehninger 8.068
Nozaki 8.931
DTASelect 8.126
Thurlkill 8.185
EMBOSS 8.2
Sillero 8.595
Patrickios 3.808
IPC_peptide 8.039
IPC2_peptide 8.083
IPC2.peptide.svr19 8.01
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2416
121
601
402.7
44.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.381 ± 0.584
2.442 ± 0.707
6.167 ± 0.327
6.871 ± 0.517
4.553 ± 0.57
8.609 ± 1.142
1.987 ± 0.211
3.684 ± 0.265
5.05 ± 0.799
8.609 ± 0.445
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.78 ± 0.326
3.56 ± 0.521
6.747 ± 0.658
3.518 ± 0.484
6.291 ± 0.714
8.195 ± 0.432
6.25 ± 0.534
6.167 ± 0.665
1.242 ± 0.291
2.897 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here