Microbacterium caowuchunii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N0THF5|A0A5N0THF5_9MICO ABC transporter permease subunit OS=Microbacterium caowuchunii OX=2614638 GN=F6B40_07245 PE=3 SV=1
MM1 pKa = 7.01TRR3 pKa = 11.84ASALTSTAILTLALAGCSGSMLAEE27 pKa = 3.88NRR29 pKa = 11.84GGDD32 pKa = 3.39QLSNLRR38 pKa = 11.84TAIEE42 pKa = 4.32SNADD46 pKa = 3.47CAEE49 pKa = 4.17LFSLLDD55 pKa = 4.37QIDD58 pKa = 4.19EE59 pKa = 4.74DD60 pKa = 4.27ADD62 pKa = 4.01AYY64 pKa = 10.23PSAQGEE70 pKa = 4.48LVNIGCFMRR79 pKa = 11.84DD80 pKa = 3.07STRR83 pKa = 11.84NDD85 pKa = 3.18ADD87 pKa = 4.12LAAQAPDD94 pKa = 4.14SPWLGVPGKK103 pKa = 9.62EE104 pKa = 4.04VEE106 pKa = 4.32PSLTCISAAEE116 pKa = 4.11TAANEE121 pKa = 4.16ADD123 pKa = 3.67STRR126 pKa = 11.84AEE128 pKa = 4.09PLIAATLDD136 pKa = 3.42SCQSVDD142 pKa = 2.52EE143 pKa = 4.31WMSVITAHH151 pKa = 6.57PGVMGMMDD159 pKa = 3.93GYY161 pKa = 10.42IPQLMDD167 pKa = 3.95LQSACYY173 pKa = 9.37SYY175 pKa = 11.47VGAAVCQDD183 pKa = 2.88ALARR187 pKa = 11.84GVRR190 pKa = 11.84VGPP193 pKa = 3.94

Molecular weight: 20.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6KR96|A0A5J6KR96_9MICO ABC transporter substrate-binding protein OS=Microbacterium caowuchunii OX=2614638 GN=F6B40_13615 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3053

0

3053

992905

31

4833

325.2

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.706 ± 0.073

0.511 ± 0.012

6.234 ± 0.036

5.691 ± 0.041

3.061 ± 0.024

9.042 ± 0.041

1.957 ± 0.026

4.466 ± 0.028

1.728 ± 0.035

10.061 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.017

1.81 ± 0.026

5.577 ± 0.038

2.666 ± 0.024

7.714 ± 0.059

5.387 ± 0.026

6.032 ± 0.041

9.073 ± 0.04

1.501 ± 0.019

1.971 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski