Psychromicrobium lacuslunae
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2887 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D4BYY9|A0A0D4BYY9_9MICC Uncharacterized protein OS=Psychromicrobium lacuslunae OX=1618207 GN=UM93_07055 PE=4 SV=1
MM1 pKa = 7.68 PKK3 pKa = 10.56 ALIIVDD9 pKa = 3.88 VQNDD13 pKa = 3.63 FCEE16 pKa = 4.91 GGSLAVSGGEE26 pKa = 3.96 EE27 pKa = 4.08 VATGISEE34 pKa = 4.84 LLDD37 pKa = 3.34 STFGAEE43 pKa = 4.06 SRR45 pKa = 11.84 EE46 pKa = 3.72 FDD48 pKa = 4.02 YY49 pKa = 11.64 VLATQDD55 pKa = 2.96 WHH57 pKa = 6.99 IDD59 pKa = 3.55 PGSHH63 pKa = 7.03 FSEE66 pKa = 4.47 TPDD69 pKa = 5.8 FIDD72 pKa = 3.23 SWPVHH77 pKa = 5.93 CVAGSKK83 pKa = 10.15 GAQLHH88 pKa = 6.53 PNLDD92 pKa = 3.59 TEE94 pKa = 4.48 YY95 pKa = 10.48 VDD97 pKa = 4.28 AYY99 pKa = 10.22 FRR101 pKa = 11.84 KK102 pKa = 10.09 GKK104 pKa = 9.54 YY105 pKa = 7.67 QAAYY109 pKa = 10.34 SGFEE113 pKa = 4.02 GFLAPEE119 pKa = 4.68 DD120 pKa = 4.12 EE121 pKa = 4.87 VPTGPRR127 pKa = 11.84 NPEE130 pKa = 3.51 QTEE133 pKa = 3.89 NPEE136 pKa = 4.03 NSEE139 pKa = 4.02 SSGDD143 pKa = 3.54 VGYY146 pKa = 10.94 DD147 pKa = 3.4 GDD149 pKa = 5.37 DD150 pKa = 4.48 DD151 pKa = 5.5 GPSLDD156 pKa = 3.46 EE157 pKa = 3.92 WLQEE161 pKa = 4.07 HH162 pKa = 6.29 EE163 pKa = 4.38 VDD165 pKa = 3.68 SVSIVGLATDD175 pKa = 3.58 YY176 pKa = 10.89 CVRR179 pKa = 11.84 ATALDD184 pKa = 3.82 AVSAGYY190 pKa = 10.01 EE191 pKa = 4.02 VTLLSEE197 pKa = 4.56 LCAGIDD203 pKa = 3.64 PAGSQAALTEE213 pKa = 4.12 LTEE216 pKa = 4.59 AGVEE220 pKa = 4.36 III222 pKa = 5.11
Molecular weight: 23.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.541
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.834
DTASelect 4.024
Thurlkill 3.579
EMBOSS 3.643
Sillero 3.859
Patrickios 0.668
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A0D4BZ54|A0A0D4BZ54_9MICC HTH tetR-type domain-containing protein OS=Psychromicrobium lacuslunae OX=1618207 GN=UM93_08905 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.71 KK12 pKa = 10.76 LPGAQIVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 8.58 NNPRR34 pKa = 11.84 MKK36 pKa = 10.38 ARR38 pKa = 11.84 QGG40 pKa = 3.24
Molecular weight: 4.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.574
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.31
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2887
0
2887
921680
37
3915
319.3
34.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.35 ± 0.062
0.577 ± 0.01
5.123 ± 0.033
5.977 ± 0.055
3.343 ± 0.032
8.411 ± 0.042
1.884 ± 0.022
4.807 ± 0.039
3.126 ± 0.041
10.957 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.832 ± 0.019
2.75 ± 0.035
5.03 ± 0.027
4.033 ± 0.03
6.065 ± 0.047
6.783 ± 0.045
5.422 ± 0.049
7.902 ± 0.04
1.464 ± 0.024
2.162 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here