Amino acid dipepetide frequency for Psychromicrobium lacuslunae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.119AlaAla: 18.119 ± 0.183	0.635AlaCys: 0.635 ± 0.029	6.395AlaAsp: 6.395 ± 0.089	9.51AlaGlu: 9.51 ± 0.156	3.472AlaPhe: 3.472 ± 0.064	10.681AlaGly: 10.681 ± 0.127	1.916AlaHis: 1.916 ± 0.044	5.643AlaIle: 5.643 ± 0.095	4.426AlaLys: 4.426 ± 0.083	13.375AlaLeu: 13.375 ± 0.171	2.439AlaMet: 2.439 ± 0.052	3.126AlaAsn: 3.126 ± 0.065	4.902AlaPro: 4.902 ± 0.088	5.116AlaGln: 5.116 ± 0.079	6.581AlaArg: 6.581 ± 0.108	7.599AlaSer: 7.599 ± 0.106	6.063AlaThr: 6.063 ± 0.093	9.95AlaVal: 9.95 ± 0.122	1.485AlaTrp: 1.485 ± 0.041	2.071AlaTyr: 2.071 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.657CysAla: 0.657 ± 0.027	0.063CysCys: 0.063 ± 0.01	0.277CysAsp: 0.277 ± 0.018	0.29CysGlu: 0.29 ± 0.018	0.222CysPhe: 0.222 ± 0.016	0.644CysGly: 0.644 ± 0.026	0.136CysHis: 0.136 ± 0.011	0.23CysIle: 0.23 ± 0.016	0.111CysLys: 0.111 ± 0.013	0.601CysLeu: 0.601 ± 0.026	0.086CysMet: 0.086 ± 0.009	0.136CysAsn: 0.136 ± 0.011	0.334CysPro: 0.334 ± 0.027	0.232CysGln: 0.232 ± 0.015	0.333CysArg: 0.333 ± 0.02	0.388CysSer: 0.388 ± 0.02	0.372CysThr: 0.372 ± 0.028	0.387CysVal: 0.387 ± 0.022	0.115CysTrp: 0.115 ± 0.011	0.155CysTyr: 0.155 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	5.991AspAla: 5.991 ± 0.085	0.306AspCys: 0.306 ± 0.019	2.665AspAsp: 2.665 ± 0.063	3.234AspGlu: 3.234 ± 0.067	1.993AspPhe: 1.993 ± 0.049	4.763AspGly: 4.763 ± 0.083	1.062AspHis: 1.062 ± 0.037	2.175AspIle: 2.175 ± 0.048	1.343AspLys: 1.343 ± 0.044	5.576AspLeu: 5.576 ± 0.089	0.706AspMet: 0.706 ± 0.028	1.146AspAsn: 1.146 ± 0.039	3.349AspPro: 3.349 ± 0.065	2.214AspGln: 2.214 ± 0.043	3.062AspArg: 3.062 ± 0.065	3.216AspSer: 3.216 ± 0.06	2.267AspThr: 2.267 ± 0.052	3.802AspVal: 3.802 ± 0.071	0.959AspTrp: 0.959 ± 0.036	1.396AspTyr: 1.396 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	7.19GluAla: 7.19 ± 0.117	0.242GluCys: 0.242 ± 0.018	2.625GluAsp: 2.625 ± 0.057	3.41GluGlu: 3.41 ± 0.075	1.968GluPhe: 1.968 ± 0.046	3.763GluGly: 3.763 ± 0.071	1.417GluHis: 1.417 ± 0.038	3.031GluIle: 3.031 ± 0.073	1.911GluLys: 1.911 ± 0.051	8.416GluLeu: 8.416 ± 0.126	1.034GluMet: 1.034 ± 0.035	1.587GluAsn: 1.587 ± 0.045	2.82GluPro: 2.82 ± 0.072	3.222GluGln: 3.222 ± 0.068	4.213GluArg: 4.213 ± 0.094	3.27GluSer: 3.27 ± 0.058	2.913GluThr: 2.913 ± 0.064	4.725GluVal: 4.725 ± 0.078	0.773GluTrp: 0.773 ± 0.027	1.243GluTyr: 1.243 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	4.039PheAla: 4.039 ± 0.067	0.271PheCys: 0.271 ± 0.017	2.307PheAsp: 2.307 ± 0.054	1.85PheGlu: 1.85 ± 0.043	1.3PhePhe: 1.3 ± 0.049	3.472PheGly: 3.472 ± 0.075	0.583PheHis: 0.583 ± 0.026	1.472PheIle: 1.472 ± 0.042	0.912PheLys: 0.912 ± 0.037	3.153PheLeu: 3.153 ± 0.064	0.518PheMet: 0.518 ± 0.025	1.024PheAsn: 1.024 ± 0.035	1.524PhePro: 1.524 ± 0.035	1.038PheGln: 1.038 ± 0.03	1.78PheArg: 1.78 ± 0.046	2.579PheSer: 2.579 ± 0.058	1.924PheThr: 1.924 ± 0.049	2.418PheVal: 2.418 ± 0.065	0.51PheTrp: 0.51 ± 0.026	0.758PheTyr: 0.758 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	8.8GlyAla: 8.8 ± 0.111	0.546GlyCys: 0.546 ± 0.026	3.64GlyAsp: 3.64 ± 0.065	4.77GlyGlu: 4.77 ± 0.075	3.426GlyPhe: 3.426 ± 0.063	6.717GlyGly: 6.717 ± 0.099	1.665GlyHis: 1.665 ± 0.046	4.51GlyIle: 4.51 ± 0.076	3.174GlyLys: 3.174 ± 0.067	9.151GlyLeu: 9.151 ± 0.128	1.755GlyMet: 1.755 ± 0.045	2.35GlyAsn: 2.35 ± 0.06	3.47GlyPro: 3.47 ± 0.077	3.293GlyGln: 3.293 ± 0.06	4.841GlyArg: 4.841 ± 0.083	6.029GlySer: 6.029 ± 0.094	4.813GlyThr: 4.813 ± 0.075	7.137GlyVal: 7.137 ± 0.102	1.619GlyTrp: 1.619 ± 0.05	2.406GlyTyr: 2.406 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	1.86HisAla: 1.86 ± 0.047	0.157HisCys: 0.157 ± 0.012	1.005HisAsp: 1.005 ± 0.033	0.925HisGlu: 0.925 ± 0.03	0.693HisPhe: 0.693 ± 0.025	1.761HisGly: 1.761 ± 0.051	0.55HisHis: 0.55 ± 0.028	0.727HisIle: 0.727 ± 0.029	0.369HisLys: 0.369 ± 0.02	2.026HisLeu: 2.026 ± 0.047	0.289HisMet: 0.289 ± 0.018	0.455HisAsn: 0.455 ± 0.021	1.353HisPro: 1.353 ± 0.039	0.931HisGln: 0.931 ± 0.029	1.544HisArg: 1.544 ± 0.044	1.227HisSer: 1.227 ± 0.037	0.962HisThr: 0.962 ± 0.034	1.129HisVal: 1.129 ± 0.036	0.358HisTrp: 0.358 ± 0.02	0.516HisTyr: 0.516 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.615IleAla: 6.615 ± 0.092	0.357IleCys: 0.357 ± 0.022	3.136IleAsp: 3.136 ± 0.065	2.947IleGlu: 2.947 ± 0.068	1.727IlePhe: 1.727 ± 0.052	4.693IleGly: 4.693 ± 0.079	0.702IleHis: 0.702 ± 0.024	2.167IleIle: 2.167 ± 0.059	1.425IleLys: 1.425 ± 0.049	4.145IleLeu: 4.145 ± 0.083	0.707IleMet: 0.707 ± 0.03	1.458IleAsn: 1.458 ± 0.042	2.398IlePro: 2.398 ± 0.047	1.319IleGln: 1.319 ± 0.038	2.578IleArg: 2.578 ± 0.056	3.638IleSer: 3.638 ± 0.067	2.725IleThr: 2.725 ± 0.057	3.718IleVal: 3.718 ± 0.069	0.578IleTrp: 0.578 ± 0.027	1.038IleTyr: 1.038 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.803LysAla: 3.803 ± 0.075	0.115LysCys: 0.115 ± 0.012	1.678LysAsp: 1.678 ± 0.047	1.522LysGlu: 1.522 ± 0.045	0.893LysPhe: 0.893 ± 0.031	2.006LysGly: 2.006 ± 0.058	0.64LysHis: 0.64 ± 0.025	1.772LysIle: 1.772 ± 0.052	1.187LysLys: 1.187 ± 0.043	3.389LysLeu: 3.389 ± 0.063	0.726LysMet: 0.726 ± 0.028	1.05LysAsn: 1.05 ± 0.041	1.902LysPro: 1.902 ± 0.054	1.171LysGln: 1.171 ± 0.038	1.827LysArg: 1.827 ± 0.048	1.881LysSer: 1.881 ± 0.054	1.967LysThr: 1.967 ± 0.052	2.667LysVal: 2.667 ± 0.062	0.311LysTrp: 0.311 ± 0.02	0.756LysTyr: 0.756 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	14.701LeuAla: 14.701 ± 0.169	0.694LeuCys: 0.694 ± 0.026	6.022LeuAsp: 6.022 ± 0.096	5.888LeuGlu: 5.888 ± 0.105	3.059LeuPhe: 3.059 ± 0.068	9.472LeuGly: 9.472 ± 0.121	1.915LeuHis: 1.915 ± 0.048	5.527LeuIle: 5.527 ± 0.108	3.218LeuLys: 3.218 ± 0.071	11.991LeuLeu: 11.991 ± 0.174	1.847LeuMet: 1.847 ± 0.049	3.102LeuAsn: 3.102 ± 0.062	5.897LeuPro: 5.897 ± 0.092	3.531LeuGln: 3.531 ± 0.067	7.102LeuArg: 7.102 ± 0.114	8.233LeuSer: 8.233 ± 0.116	5.808LeuThr: 5.808 ± 0.08	8.403LeuVal: 8.403 ± 0.119	1.377LeuTrp: 1.377 ± 0.038	1.787LeuTyr: 1.787 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.237MetAla: 2.237 ± 0.044	0.097MetCys: 0.097 ± 0.01	0.771MetAsp: 0.771 ± 0.03	0.737MetGlu: 0.737 ± 0.029	0.509MetPhe: 0.509 ± 0.024	1.341MetGly: 1.341 ± 0.037	0.317MetHis: 0.317 ± 0.016	0.963MetIle: 0.963 ± 0.033	0.677MetLys: 0.677 ± 0.025	1.956MetLeu: 1.956 ± 0.046	0.353MetMet: 0.353 ± 0.021	0.624MetAsn: 0.624 ± 0.028	1.064MetPro: 1.064 ± 0.039	0.607MetGln: 0.607 ± 0.027	1.093MetArg: 1.093 ± 0.036	1.573MetSer: 1.573 ± 0.04	1.458MetThr: 1.458 ± 0.034	1.473MetVal: 1.473 ± 0.042	0.157MetTrp: 0.157 ± 0.015	0.314MetTyr: 0.314 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.009AsnAla: 3.009 ± 0.063	0.199AsnCys: 0.199 ± 0.018	1.337AsnAsp: 1.337 ± 0.038	1.229AsnGlu: 1.229 ± 0.038	1.039AsnPhe: 1.039 ± 0.036	2.53AsnGly: 2.53 ± 0.07	0.542AsnHis: 0.542 ± 0.026	1.289AsnIle: 1.289 ± 0.045	0.804AsnLys: 0.804 ± 0.035	2.651AsnLeu: 2.651 ± 0.051	0.468AsnMet: 0.468 ± 0.021	0.92AsnAsn: 0.92 ± 0.042	1.992AsnPro: 1.992 ± 0.044	1.154AsnGln: 1.154 ± 0.039	1.604AsnArg: 1.604 ± 0.047	1.989AsnSer: 1.989 ± 0.055	1.694AsnThr: 1.694 ± 0.068	1.761AsnVal: 1.761 ± 0.05	0.488AsnTrp: 0.488 ± 0.022	0.806AsnTyr: 0.806 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	6.49ProAla: 6.49 ± 0.107	0.188ProCys: 0.188 ± 0.014	2.772ProAsp: 2.772 ± 0.057	4.215ProGlu: 4.215 ± 0.079	1.625ProPhe: 1.625 ± 0.046	4.792ProGly: 4.792 ± 0.082	0.927ProHis: 0.927 ± 0.034	2.095ProIle: 2.095 ± 0.052	1.471ProLys: 1.471 ± 0.039	4.988ProLeu: 4.988 ± 0.076	0.846ProMet: 0.846 ± 0.028	1.307ProAsn: 1.307 ± 0.037	1.727ProPro: 1.727 ± 0.05	2.084ProGln: 2.084 ± 0.055	2.569ProArg: 2.569 ± 0.06	3.498ProSer: 3.498 ± 0.068	2.678ProThr: 2.678 ± 0.059	4.252ProVal: 4.252 ± 0.08	0.757ProTrp: 0.757 ± 0.03	1.014ProTyr: 1.014 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	5.01GlnAla: 5.01 ± 0.094	0.186GlnCys: 0.186 ± 0.012	1.737GlnAsp: 1.737 ± 0.05	1.949GlnGlu: 1.949 ± 0.052	1.089GlnPhe: 1.089 ± 0.037	2.948GlnGly: 2.948 ± 0.065	0.946GlnHis: 0.946 ± 0.034	1.972GlnIle: 1.972 ± 0.042	1.191GlnLys: 1.191 ± 0.038	5.145GlnLeu: 5.145 ± 0.087	0.733GlnMet: 0.733 ± 0.029	1.095GlnAsn: 1.095 ± 0.037	2.213GlnPro: 2.213 ± 0.062	2.567GlnGln: 2.567 ± 0.067	3.029GlnArg: 3.029 ± 0.064	2.271GlnSer: 2.271 ± 0.056	1.866GlnThr: 1.866 ± 0.044	2.974GlnVal: 2.974 ± 0.058	0.609GlnTrp: 0.609 ± 0.025	0.801GlnTyr: 0.801 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	6.567ArgAla: 6.567 ± 0.103	0.322ArgCys: 0.322 ± 0.023	2.931ArgAsp: 2.931 ± 0.063	3.892ArgGlu: 3.892 ± 0.08	2.239ArgPhe: 2.239 ± 0.05	4.339ArgGly: 4.339 ± 0.078	1.236ArgHis: 1.236 ± 0.046	3.09ArgIle: 3.09 ± 0.055	1.938ArgLys: 1.938 ± 0.045	6.638ArgLeu: 6.638 ± 0.097	1.317ArgMet: 1.317 ± 0.039	1.642ArgAsn: 1.642 ± 0.041	2.856ArgPro: 2.856 ± 0.066	2.665ArgGln: 2.665 ± 0.056	4.808ArgArg: 4.808 ± 0.086	3.976ArgSer: 3.976 ± 0.077	3.148ArgThr: 3.148 ± 0.064	4.402ArgVal: 4.402 ± 0.075	1.131ArgTrp: 1.131 ± 0.035	1.519ArgTyr: 1.519 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	8.412SerAla: 8.412 ± 0.092	0.414SerCys: 0.414 ± 0.031	2.98SerAsp: 2.98 ± 0.058	3.75SerGlu: 3.75 ± 0.064	2.478SerPhe: 2.478 ± 0.049	6.443SerGly: 6.443 ± 0.101	1.176SerHis: 1.176 ± 0.035	3.106SerIle: 3.106 ± 0.057	2.074SerLys: 2.074 ± 0.055	6.622SerLeu: 6.622 ± 0.085	1.427SerMet: 1.427 ± 0.043	1.937SerAsn: 1.937 ± 0.056	3.155SerPro: 3.155 ± 0.063	2.487SerGln: 2.487 ± 0.057	3.882SerArg: 3.882 ± 0.079	5.225SerSer: 5.225 ± 0.097	3.978SerThr: 3.978 ± 0.077	5.202SerVal: 5.202 ± 0.09	1.285SerTrp: 1.285 ± 0.041	1.799SerTyr: 1.799 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	6.995ThrAla: 6.995 ± 0.105	0.234ThrCys: 0.234 ± 0.018	2.784ThrAsp: 2.784 ± 0.057	3.535ThrGlu: 3.535 ± 0.07	1.657ThrPhe: 1.657 ± 0.044	4.925ThrGly: 4.925 ± 0.078	0.889ThrHis: 0.889 ± 0.028	2.585ThrIle: 2.585 ± 0.058	1.72ThrLys: 1.72 ± 0.047	5.781ThrLeu: 5.781 ± 0.089	1.0ThrMet: 1.0 ± 0.036	1.333ThrAsn: 1.333 ± 0.052	3.328ThrPro: 3.328 ± 0.068	1.699ThrGln: 1.699 ± 0.043	2.393ThrArg: 2.393 ± 0.055	3.55ThrSer: 3.55 ± 0.075	3.022ThrThr: 3.022 ± 0.09	5.288ThrVal: 5.288 ± 0.11	0.545ThrTrp: 0.545 ± 0.028	0.959ThrTyr: 0.959 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	9.249ValAla: 9.249 ± 0.129	0.476ValCys: 0.476 ± 0.02	4.349ValAsp: 4.349 ± 0.081	4.544ValGlu: 4.544 ± 0.071	2.585ValPhe: 2.585 ± 0.059	5.851ValGly: 5.851 ± 0.095	1.543ValHis: 1.543 ± 0.042	4.282ValIle: 4.282 ± 0.075	2.411ValLys: 2.411 ± 0.063	9.254ValLeu: 9.254 ± 0.118	1.519ValMet: 1.519 ± 0.041	2.159ValAsn: 2.159 ± 0.061	4.084ValPro: 4.084 ± 0.083	2.798ValGln: 2.798 ± 0.056	4.664ValArg: 4.664 ± 0.079	5.423ValSer: 5.423 ± 0.084	4.618ValThr: 4.618 ± 0.108	6.953ValVal: 6.953 ± 0.102	0.874ValTrp: 0.874 ± 0.035	1.386ValTyr: 1.386 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.464TrpAla: 1.464 ± 0.04	0.113TrpCys: 0.113 ± 0.012	0.698TrpAsp: 0.698 ± 0.031	0.588TrpGlu: 0.588 ± 0.026	0.559TrpPhe: 0.559 ± 0.028	1.055TrpGly: 1.055 ± 0.037	0.299TrpHis: 0.299 ± 0.021	0.684TrpIle: 0.684 ± 0.03	0.418TrpLys: 0.418 ± 0.022	1.994TrpLeu: 1.994 ± 0.056	0.294TrpMet: 0.294 ± 0.019	0.478TrpAsn: 0.478 ± 0.027	0.715TrpPro: 0.715 ± 0.031	0.936TrpGln: 0.936 ± 0.034	1.056TrpArg: 1.056 ± 0.033	0.873TrpSer: 0.873 ± 0.027	0.762TrpThr: 0.762 ± 0.031	0.999TrpVal: 0.999 ± 0.033	0.316TrpTrp: 0.316 ± 0.018	0.336TrpTyr: 0.336 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.294TyrAla: 2.294 ± 0.047	0.155TyrCys: 0.155 ± 0.013	1.12TyrAsp: 1.12 ± 0.049	0.989TyrGlu: 0.989 ± 0.034	0.898TyrPhe: 0.898 ± 0.035	1.918TyrGly: 1.918 ± 0.047	0.376TyrHis: 0.376 ± 0.021	0.749TyrIle: 0.749 ± 0.032	0.486TyrLys: 0.486 ± 0.024	2.723TyrLeu: 2.723 ± 0.055	0.256TyrMet: 0.256 ± 0.017	0.575TyrAsn: 0.575 ± 0.028	1.214TyrPro: 1.214 ± 0.041	1.267TyrGln: 1.267 ± 0.044	1.694TyrArg: 1.694 ± 0.045	1.392TyrSer: 1.392 ± 0.04	1.186TyrThr: 1.186 ± 0.041	1.38TyrVal: 1.38 ± 0.038	0.39TyrTrp: 0.39 ± 0.024	0.553TyrTyr: 0.553 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2887 proteins (921680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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