Clostridium sp. CT7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M8T7Q0|A0A2M8T7Q0_9CLOT Uncharacterized protein OS=Clostridium sp. CT7 OX=2052574 GN=CUB90_03675 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.22KK3 pKa = 9.99YY4 pKa = 9.92VCVVCGYY11 pKa = 10.11IYY13 pKa = 10.67DD14 pKa = 4.23PAEE17 pKa = 4.34GDD19 pKa = 3.65PDD21 pKa = 4.21NGVQPGTSFEE31 pKa = 4.87NIPDD35 pKa = 3.65DD36 pKa = 3.92WTCPLCGVGKK46 pKa = 9.96DD47 pKa = 3.61QFEE50 pKa = 4.41EE51 pKa = 4.14AQEE54 pKa = 3.91

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M8T6K9|A0A2M8T6K9_9CLOT tRNA(Met) cytidine acetate ligase OS=Clostridium sp. CT7 OX=2052574 GN=tmcAL PE=3 SV=1
MM1 pKa = 7.48SRR3 pKa = 11.84KK4 pKa = 9.55CDD6 pKa = 3.06ICGKK10 pKa = 10.14GLVAGVQYY18 pKa = 10.89SHH20 pKa = 6.55SHH22 pKa = 5.37RR23 pKa = 11.84QSKK26 pKa = 7.96RR27 pKa = 11.84TWLPNIRR34 pKa = 11.84KK35 pKa = 9.04IKK37 pKa = 10.35AVVDD41 pKa = 3.91GTPKK45 pKa = 9.94TIHH48 pKa = 5.48VCTRR52 pKa = 11.84CLRR55 pKa = 11.84SGKK58 pKa = 7.45VQRR61 pKa = 11.84ALL63 pKa = 3.45

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4078

0

4078

1301812

25

6883

319.2

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.692 ± 0.037

1.298 ± 0.014

5.636 ± 0.031

6.545 ± 0.05

4.501 ± 0.03

6.303 ± 0.041

1.359 ± 0.014

9.707 ± 0.043

9.56 ± 0.04

8.603 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.019

6.654 ± 0.041

2.728 ± 0.022

2.262 ± 0.02

3.191 ± 0.025

6.764 ± 0.039

4.911 ± 0.036

6.57 ± 0.03

0.729 ± 0.011

4.284 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski