Amino acid dipepetide frequency for Clostridium sp. CT7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.659AlaAla: 4.659 ± 0.081
0.73AlaCys: 0.73 ± 0.024
2.774AlaAsp: 2.774 ± 0.047
3.39AlaGlu: 3.39 ± 0.058
2.679AlaPhe: 2.679 ± 0.052
3.917AlaGly: 3.917 ± 0.084
0.837AlaHis: 0.837 ± 0.028
5.269AlaIle: 5.269 ± 0.078
4.689AlaLys: 4.689 ± 0.064
5.55AlaLeu: 5.55 ± 0.077
1.508AlaMet: 1.508 ± 0.038
2.723AlaAsn: 2.723 ± 0.054
1.444AlaPro: 1.444 ± 0.038
1.384AlaGln: 1.384 ± 0.026
1.824AlaArg: 1.824 ± 0.042
3.855AlaSer: 3.855 ± 0.06
2.382AlaThr: 2.382 ± 0.061
4.622AlaVal: 4.622 ± 0.065
0.412AlaTrp: 0.412 ± 0.019
2.277AlaTyr: 2.277 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.716CysAla: 0.716 ± 0.025
0.214CysCys: 0.214 ± 0.013
0.742CysAsp: 0.742 ± 0.024
0.744CysGlu: 0.744 ± 0.022
0.617CysPhe: 0.617 ± 0.021
1.128CysGly: 1.128 ± 0.037
0.227CysHis: 0.227 ± 0.012
1.316CysIle: 1.316 ± 0.033
1.078CysLys: 1.078 ± 0.031
0.98CysLeu: 0.98 ± 0.027
0.338CysMet: 0.338 ± 0.016
0.744CysAsn: 0.744 ± 0.026
0.455CysPro: 0.455 ± 0.02
0.224CysGln: 0.224 ± 0.013
0.413CysArg: 0.413 ± 0.017
0.976CysSer: 0.976 ± 0.031
0.683CysThr: 0.683 ± 0.026
0.78CysVal: 0.78 ± 0.028
0.08CysTrp: 0.08 ± 0.008
0.529CysTyr: 0.529 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
2.848AspAla: 2.848 ± 0.049
0.636AspCys: 0.636 ± 0.022
3.23AspAsp: 3.23 ± 0.062
4.546AspGlu: 4.546 ± 0.071
2.778AspPhe: 2.778 ± 0.056
3.638AspGly: 3.638 ± 0.086
0.549AspHis: 0.549 ± 0.02
5.957AspIle: 5.957 ± 0.069
5.71AspLys: 5.71 ± 0.069
4.382AspLeu: 4.382 ± 0.064
1.599AspMet: 1.599 ± 0.037
3.567AspAsn: 3.567 ± 0.061
1.304AspPro: 1.304 ± 0.033
0.76AspGln: 0.76 ± 0.026
1.721AspArg: 1.721 ± 0.038
3.426AspSer: 3.426 ± 0.056
2.68AspThr: 2.68 ± 0.055
3.832AspVal: 3.832 ± 0.059
0.446AspTrp: 0.446 ± 0.026
2.752AspTyr: 2.752 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
3.898GluAla: 3.898 ± 0.064
0.734GluCys: 0.734 ± 0.026
3.792GluAsp: 3.792 ± 0.067
5.187GluGlu: 5.187 ± 0.101
2.77GluPhe: 2.77 ± 0.049
3.275GluGly: 3.275 ± 0.056
0.873GluHis: 0.873 ± 0.024
6.479GluIle: 6.479 ± 0.095
7.344GluLys: 7.344 ± 0.098
5.961GluLeu: 5.961 ± 0.082
1.751GluMet: 1.751 ± 0.039
5.098GluAsn: 5.098 ± 0.08
1.336GluPro: 1.336 ± 0.037
1.406GluGln: 1.406 ± 0.034
2.212GluArg: 2.212 ± 0.04
3.088GluSer: 3.088 ± 0.053
2.755GluThr: 2.755 ± 0.052
4.238GluVal: 4.238 ± 0.059
0.416GluTrp: 0.416 ± 0.017
2.835GluTyr: 2.835 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.375PheAla: 2.375 ± 0.046
0.589PheCys: 0.589 ± 0.024
2.597PheAsp: 2.597 ± 0.051
2.632PheGlu: 2.632 ± 0.053
2.099PhePhe: 2.099 ± 0.043
2.77PheGly: 2.77 ± 0.058
0.631PheHis: 0.631 ± 0.021
4.512PheIle: 4.512 ± 0.067
4.173PheLys: 4.173 ± 0.059
3.915PheLeu: 3.915 ± 0.076
1.294PheMet: 1.294 ± 0.029
3.168PheAsn: 3.168 ± 0.052
1.201PhePro: 1.201 ± 0.03
0.979PheGln: 0.979 ± 0.028
1.254PheArg: 1.254 ± 0.03
3.404PheSer: 3.404 ± 0.06
2.248PheThr: 2.248 ± 0.046
2.878PheVal: 2.878 ± 0.061
0.32PheTrp: 0.32 ± 0.015
1.97PheTyr: 1.97 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
3.974GlyAla: 3.974 ± 0.076
0.92GlyCys: 0.92 ± 0.031
3.21GlyAsp: 3.21 ± 0.059
3.845GlyGlu: 3.845 ± 0.055
3.061GlyPhe: 3.061 ± 0.044
4.114GlyGly: 4.114 ± 0.091
0.971GlyHis: 0.971 ± 0.031
6.705GlyIle: 6.705 ± 0.085
5.89GlyLys: 5.89 ± 0.08
4.905GlyLeu: 4.905 ± 0.073
1.783GlyMet: 1.783 ± 0.039
3.551GlyAsn: 3.551 ± 0.083
1.106GlyPro: 1.106 ± 0.03
1.507GlyGln: 1.507 ± 0.039
2.086GlyArg: 2.086 ± 0.046
3.826GlySer: 3.826 ± 0.079
3.725GlyThr: 3.725 ± 0.085
4.316GlyVal: 4.316 ± 0.068
0.594GlyTrp: 0.594 ± 0.028
2.939GlyTyr: 2.939 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
0.636HisAla: 0.636 ± 0.024
0.216HisCys: 0.216 ± 0.012
0.797HisAsp: 0.797 ± 0.023
0.899HisGlu: 0.899 ± 0.031
0.641HisPhe: 0.641 ± 0.02
1.047HisGly: 1.047 ± 0.032
0.308HisHis: 0.308 ± 0.02
1.255HisIle: 1.255 ± 0.03
1.101HisLys: 1.101 ± 0.03
1.051HisLeu: 1.051 ± 0.033
0.378HisMet: 0.378 ± 0.018
0.793HisAsn: 0.793 ± 0.028
0.594HisPro: 0.594 ± 0.022
0.285HisGln: 0.285 ± 0.015
0.485HisArg: 0.485 ± 0.02
0.854HisSer: 0.854 ± 0.027
0.735HisThr: 0.735 ± 0.028
0.837HisVal: 0.837 ± 0.027
0.111HisTrp: 0.111 ± 0.009
0.572HisTyr: 0.572 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.582IleAla: 5.582 ± 0.072
1.448IleCys: 1.448 ± 0.038
5.672IleAsp: 5.672 ± 0.074
6.276IleGlu: 6.276 ± 0.089
4.476IlePhe: 4.476 ± 0.079
5.909IleGly: 5.909 ± 0.079
1.224IleHis: 1.224 ± 0.033
9.13IleIle: 9.13 ± 0.122
9.19IleLys: 9.19 ± 0.094
8.814IleLeu: 8.814 ± 0.107
2.46IleMet: 2.46 ± 0.049
6.426IleAsn: 6.426 ± 0.08
3.085IlePro: 3.085 ± 0.05
1.987IleGln: 1.987 ± 0.042
2.967IleArg: 2.967 ± 0.054
7.254IleSer: 7.254 ± 0.092
4.766IleThr: 4.766 ± 0.07
6.09IleVal: 6.09 ± 0.074
0.59IleTrp: 0.59 ± 0.022
3.724IleTyr: 3.724 ± 0.062
0.0IleXaa: 0.0 ± 0.0
Lys
5.172LysAla: 5.172 ± 0.076
1.092LysCys: 1.092 ± 0.032
6.112LysAsp: 6.112 ± 0.076
7.243LysGlu: 7.243 ± 0.105
3.736LysPhe: 3.736 ± 0.057
5.151LysGly: 5.151 ± 0.081
1.203LysHis: 1.203 ± 0.031
8.75LysIle: 8.75 ± 0.1
9.349LysLys: 9.349 ± 0.094
8.5LysLeu: 8.5 ± 0.094
2.708LysMet: 2.708 ± 0.044
7.399LysAsn: 7.399 ± 0.095
2.191LysPro: 2.191 ± 0.04
2.201LysGln: 2.201 ± 0.042
3.118LysArg: 3.118 ± 0.062
5.86LysSer: 5.86 ± 0.074
4.275LysThr: 4.275 ± 0.057
6.346LysVal: 6.346 ± 0.069
0.663LysTrp: 0.663 ± 0.023
4.531LysTyr: 4.531 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
4.725LeuAla: 4.725 ± 0.065
1.229LeuCys: 1.229 ± 0.029
4.556LeuAsp: 4.556 ± 0.069
4.86LeuGlu: 4.86 ± 0.086
3.626LeuPhe: 3.626 ± 0.067
5.533LeuGly: 5.533 ± 0.065
1.074LeuHis: 1.074 ± 0.031
8.017LeuIle: 8.017 ± 0.111
9.378LeuLys: 9.378 ± 0.106
6.885LeuLeu: 6.885 ± 0.088
2.371LeuMet: 2.371 ± 0.051
6.231LeuAsn: 6.231 ± 0.09
2.636LeuPro: 2.636 ± 0.047
2.151LeuGln: 2.151 ± 0.047
2.871LeuArg: 2.871 ± 0.049
6.648LeuSer: 6.648 ± 0.086
4.398LeuThr: 4.398 ± 0.07
5.051LeuVal: 5.051 ± 0.066
0.571LeuTrp: 0.571 ± 0.026
3.217LeuTyr: 3.217 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
1.648MetAla: 1.648 ± 0.035
0.309MetCys: 0.309 ± 0.016
1.629MetAsp: 1.629 ± 0.041
1.698MetGlu: 1.698 ± 0.041
1.112MetPhe: 1.112 ± 0.032
1.758MetGly: 1.758 ± 0.038
0.395MetHis: 0.395 ± 0.017
2.286MetIle: 2.286 ± 0.043
2.725MetLys: 2.725 ± 0.049
2.43MetLeu: 2.43 ± 0.041
0.705MetMet: 0.705 ± 0.02
1.946MetAsn: 1.946 ± 0.037
0.958MetPro: 0.958 ± 0.022
0.733MetGln: 0.733 ± 0.024
0.891MetArg: 0.891 ± 0.032
1.843MetSer: 1.843 ± 0.035
1.163MetThr: 1.163 ± 0.029
1.572MetVal: 1.572 ± 0.039
0.188MetTrp: 0.188 ± 0.013
1.035MetTyr: 1.035 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.269AsnAla: 3.269 ± 0.049
0.873AsnCys: 0.873 ± 0.032
3.558AsnAsp: 3.558 ± 0.061
4.278AsnGlu: 4.278 ± 0.067
2.904AsnPhe: 2.904 ± 0.055
4.119AsnGly: 4.119 ± 0.076
0.79AsnHis: 0.79 ± 0.029
7.104AsnIle: 7.104 ± 0.084
6.517AsnLys: 6.517 ± 0.083
5.803AsnLeu: 5.803 ± 0.08
1.811AsnMet: 1.811 ± 0.033
4.583AsnAsn: 4.583 ± 0.074
2.105AsnPro: 2.105 ± 0.044
1.314AsnGln: 1.314 ± 0.034
1.952AsnArg: 1.952 ± 0.041
4.656AsnSer: 4.656 ± 0.076
3.171AsnThr: 3.171 ± 0.053
4.286AsnVal: 4.286 ± 0.063
0.488AsnTrp: 0.488 ± 0.022
2.963AsnTyr: 2.963 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
1.42ProAla: 1.42 ± 0.038
0.34ProCys: 0.34 ± 0.016
1.585ProAsp: 1.585 ± 0.035
2.046ProGlu: 2.046 ± 0.039
1.376ProPhe: 1.376 ± 0.032
1.613ProGly: 1.613 ± 0.039
0.45ProHis: 0.45 ± 0.021
2.629ProIle: 2.629 ± 0.049
2.285ProLys: 2.285 ± 0.043
2.251ProLeu: 2.251 ± 0.039
0.678ProMet: 0.678 ± 0.024
1.608ProAsn: 1.608 ± 0.038
0.606ProPro: 0.606 ± 0.021
0.787ProGln: 0.787 ± 0.025
0.76ProArg: 0.76 ± 0.029
1.735ProSer: 1.735 ± 0.037
1.473ProThr: 1.473 ± 0.035
2.086ProVal: 2.086 ± 0.042
0.237ProTrp: 0.237 ± 0.013
1.32ProTyr: 1.32 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
1.296GlnAla: 1.296 ± 0.035
0.257GlnCys: 0.257 ± 0.013
1.267GlnAsp: 1.267 ± 0.038
1.38GlnGlu: 1.38 ± 0.04
0.981GlnPhe: 0.981 ± 0.028
1.306GlnGly: 1.306 ± 0.034
0.311GlnHis: 0.311 ± 0.016
2.154GlnIle: 2.154 ± 0.041
2.212GlnLys: 2.212 ± 0.041
1.811GlnLeu: 1.811 ± 0.041
0.687GlnMet: 0.687 ± 0.023
1.884GlnAsn: 1.884 ± 0.047
0.554GlnPro: 0.554 ± 0.021
0.618GlnGln: 0.618 ± 0.026
0.817GlnArg: 0.817 ± 0.033
1.421GlnSer: 1.421 ± 0.036
1.041GlnThr: 1.041 ± 0.034
1.352GlnVal: 1.352 ± 0.035
0.178GlnTrp: 0.178 ± 0.012
1.091GlnTyr: 1.091 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
1.788ArgAla: 1.788 ± 0.035
0.407ArgCys: 0.407 ± 0.018
1.665ArgAsp: 1.665 ± 0.037
2.563ArgGlu: 2.563 ± 0.049
1.363ArgPhe: 1.363 ± 0.036
1.857ArgGly: 1.857 ± 0.04
0.475ArgHis: 0.475 ± 0.019
3.157ArgIle: 3.157 ± 0.047
3.181ArgLys: 3.181 ± 0.062
2.569ArgLeu: 2.569 ± 0.052
0.912ArgMet: 0.912 ± 0.031
2.115ArgAsn: 2.115 ± 0.04
0.781ArgPro: 0.781 ± 0.026
0.869ArgGln: 0.869 ± 0.03
1.327ArgArg: 1.327 ± 0.04
1.649ArgSer: 1.649 ± 0.033
1.546ArgThr: 1.546 ± 0.035
2.039ArgVal: 2.039 ± 0.041
0.256ArgTrp: 0.256 ± 0.015
1.392ArgTyr: 1.392 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
3.57SerAla: 3.57 ± 0.07
0.774SerCys: 0.774 ± 0.023
3.667SerAsp: 3.667 ± 0.064
4.216SerGlu: 4.216 ± 0.083
3.103SerPhe: 3.103 ± 0.051
4.747SerGly: 4.747 ± 0.083
0.894SerHis: 0.894 ± 0.031
6.936SerIle: 6.936 ± 0.087
6.419SerLys: 6.419 ± 0.072
5.595SerLeu: 5.595 ± 0.074
1.862SerMet: 1.862 ± 0.041
4.247SerAsn: 4.247 ± 0.079
1.624SerPro: 1.624 ± 0.037
1.675SerGln: 1.675 ± 0.041
2.049SerArg: 2.049 ± 0.042
5.166SerSer: 5.166 ± 0.106
3.445SerThr: 3.445 ± 0.067
4.329SerVal: 4.329 ± 0.064
0.51SerTrp: 0.51 ± 0.025
2.807SerTyr: 2.807 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
3.272ThrAla: 3.272 ± 0.063
0.567ThrCys: 0.567 ± 0.019
2.566ThrAsp: 2.566 ± 0.06
2.71ThrGlu: 2.71 ± 0.051
2.185ThrPhe: 2.185 ± 0.045
3.683ThrGly: 3.683 ± 0.098
0.742ThrHis: 0.742 ± 0.026
4.611ThrIle: 4.611 ± 0.062
3.757ThrLys: 3.757 ± 0.052
4.533ThrLeu: 4.533 ± 0.06
1.12ThrMet: 1.12 ± 0.027
2.859ThrAsn: 2.859 ± 0.051
1.78ThrPro: 1.78 ± 0.037
1.072ThrGln: 1.072 ± 0.033
1.471ThrArg: 1.471 ± 0.035
3.535ThrSer: 3.535 ± 0.075
2.655ThrThr: 2.655 ± 0.062
3.588ThrVal: 3.588 ± 0.073
0.395ThrTrp: 0.395 ± 0.02
2.012ThrTyr: 2.012 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
3.525ValAla: 3.525 ± 0.063
0.952ValCys: 0.952 ± 0.029
3.843ValAsp: 3.843 ± 0.063
3.913ValGlu: 3.913 ± 0.059
3.004ValPhe: 3.004 ± 0.052
4.171ValGly: 4.171 ± 0.074
0.905ValHis: 0.905 ± 0.028
6.132ValIle: 6.132 ± 0.082
5.985ValLys: 5.985 ± 0.076
5.913ValLeu: 5.913 ± 0.077
1.698ValMet: 1.698 ± 0.039
4.107ValAsn: 4.107 ± 0.072
2.159ValPro: 2.159 ± 0.048
1.649ValGln: 1.649 ± 0.039
1.995ValArg: 1.995 ± 0.037
4.846ValSer: 4.846 ± 0.067
3.554ValThr: 3.554 ± 0.079
4.437ValVal: 4.437 ± 0.068
0.419ValTrp: 0.419 ± 0.021
2.498ValTyr: 2.498 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.385TrpAla: 0.385 ± 0.018
0.11TrpCys: 0.11 ± 0.008
0.422TrpAsp: 0.422 ± 0.021
0.38TrpGlu: 0.38 ± 0.019
0.346TrpPhe: 0.346 ± 0.018
0.547TrpGly: 0.547 ± 0.022
0.149TrpHis: 0.149 ± 0.01
0.656TrpIle: 0.656 ± 0.023
0.585TrpLys: 0.585 ± 0.021
0.624TrpLeu: 0.624 ± 0.022
0.233TrpMet: 0.233 ± 0.014
0.53TrpAsn: 0.53 ± 0.021
0.15TrpPro: 0.15 ± 0.011
0.28TrpGln: 0.28 ± 0.023
0.236TrpArg: 0.236 ± 0.015
0.459TrpSer: 0.459 ± 0.027
0.367TrpThr: 0.367 ± 0.019
0.417TrpVal: 0.417 ± 0.02
0.086TrpTrp: 0.086 ± 0.009
0.324TrpTyr: 0.324 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.164TyrAla: 2.164 ± 0.042
0.585TyrCys: 0.585 ± 0.023
2.672TyrAsp: 2.672 ± 0.047
2.643TyrGlu: 2.643 ± 0.058
2.152TyrPhe: 2.152 ± 0.044
2.746TyrGly: 2.746 ± 0.055
0.587TyrHis: 0.587 ± 0.024
4.015TyrIle: 4.015 ± 0.059
4.03TyrLys: 4.03 ± 0.064
3.56TyrLeu: 3.56 ± 0.057
1.128TyrMet: 1.128 ± 0.031
2.964TyrAsn: 2.964 ± 0.054
1.216TyrPro: 1.216 ± 0.032
0.737TyrGln: 0.737 ± 0.025
1.463TyrArg: 1.463 ± 0.037
3.136TyrSer: 3.136 ± 0.052
2.052TyrThr: 2.052 ± 0.04
2.609TyrVal: 2.609 ± 0.047
0.329TyrTrp: 0.329 ± 0.016
2.049TyrTyr: 2.049 ± 0.057
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4078 proteins (1301812 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski