Avian paramyxovirus UPO216

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Avulavirinae; Orthoavulavirus; Avian orthoavulavirus 16

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z1G744|A0A1Z1G744_9MONO Nucleocapsid OS=Avian paramyxovirus UPO216 OX=2006690 GN=NP PE=3 SV=1
MM1 pKa = 7.7IFTMYY6 pKa = 10.3HH7 pKa = 4.07VTVLLLLSLLTLPLGIQLARR27 pKa = 11.84ASIDD31 pKa = 3.23GRR33 pKa = 11.84QLAAAGIVVTGEE45 pKa = 3.95KK46 pKa = 10.28AINLYY51 pKa = 9.85TSSQTGTIVVKK62 pKa = 10.29LLPNVPQGRR71 pKa = 11.84EE72 pKa = 3.49ACMRR76 pKa = 11.84DD77 pKa = 3.35PLTSYY82 pKa = 11.45NKK84 pKa = 9.43TLTSLLSPLGEE95 pKa = 4.44AIRR98 pKa = 11.84RR99 pKa = 11.84IHH101 pKa = 6.94EE102 pKa = 4.49STTEE106 pKa = 3.89TAGLVQARR114 pKa = 11.84LVGAIIGSVALGVATSAQITAAAALIQANKK144 pKa = 9.49NAEE147 pKa = 4.37NILKK151 pKa = 10.2LKK153 pKa = 10.52QSIAATNEE161 pKa = 3.69AVHH164 pKa = 6.12EE165 pKa = 4.43VTDD168 pKa = 4.28GLSQLAVAVGKK179 pKa = 8.56MQDD182 pKa = 4.1FINTQFNNTAQEE194 pKa = 3.9IDD196 pKa = 4.56CIRR199 pKa = 11.84ISQQLGVEE207 pKa = 4.12LNLYY211 pKa = 8.46LTEE214 pKa = 4.14LTTVFGPQITSPALSPLSIQALYY237 pKa = 10.83NLAGGNLDD245 pKa = 3.61VLLSKK250 pKa = 10.64IGVGNNQLSALISSGLISGSPILYY274 pKa = 10.22DD275 pKa = 3.89SQTQLLGIQVTLPSVSSLNNMRR297 pKa = 11.84AIFLEE302 pKa = 4.17TLSVSTDD309 pKa = 2.87KK310 pKa = 11.47GFAAALIPKK319 pKa = 9.39VVTTVGTVTEE329 pKa = 4.37EE330 pKa = 4.68LDD332 pKa = 3.11TSYY335 pKa = 11.1CIEE338 pKa = 3.86TDD340 pKa = 2.54IDD342 pKa = 4.24LFCTRR347 pKa = 11.84IVTFPMSPGIYY358 pKa = 10.03ACLNGNTSEE367 pKa = 4.19CMYY370 pKa = 10.92SKK372 pKa = 9.2TQGALTTPYY381 pKa = 9.5MSVKK385 pKa = 10.27GSIVANCKK393 pKa = 7.6MTTCRR398 pKa = 11.84CADD401 pKa = 3.65PASIISQNYY410 pKa = 8.04GEE412 pKa = 4.78AVSLIDD418 pKa = 4.09SSVCRR423 pKa = 11.84VITLDD428 pKa = 3.45GVTLRR433 pKa = 11.84LSGSFDD439 pKa = 2.97STYY442 pKa = 10.95QKK444 pKa = 11.11NITIRR449 pKa = 11.84DD450 pKa = 3.69SQVIITGSLDD460 pKa = 2.88ISTEE464 pKa = 3.79LGNVNNSINNALDD477 pKa = 3.98KK478 pKa = 10.92IEE480 pKa = 4.61EE481 pKa = 4.35SNQILEE487 pKa = 4.41SVNVSLTSTNALIVYY502 pKa = 7.62IICTALALICGITGLILSCYY522 pKa = 9.89IMYY525 pKa = 11.01KK526 pKa = 9.36MRR528 pKa = 11.84SQQKK532 pKa = 7.67TLMWLGNNTLDD543 pKa = 4.73QMRR546 pKa = 11.84AQTKK550 pKa = 6.77MM551 pKa = 3.14

Molecular weight: 58.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z1G749|A0A1Z1G749_9MONO Matrix protein OS=Avian paramyxovirus UPO216 OX=2006690 GN=M PE=4 SV=1
MM1 pKa = 8.22DD2 pKa = 3.47STRR5 pKa = 11.84TIGIHH10 pKa = 6.85FDD12 pKa = 3.35PTLPASSLLAFPIVLQEE29 pKa = 4.06TGDD32 pKa = 3.69GKK34 pKa = 11.03KK35 pKa = 10.19QITPQYY41 pKa = 9.77RR42 pKa = 11.84IQKK45 pKa = 8.68LDD47 pKa = 2.89SWTEE51 pKa = 3.94SKK53 pKa = 10.89DD54 pKa = 3.28DD55 pKa = 3.62SLFITTYY62 pKa = 10.76GFIFRR67 pKa = 11.84VGGGGSNMGTIEE79 pKa = 4.01GTPTRR84 pKa = 11.84EE85 pKa = 3.98LLSAAMLCIGSVSNTTDD102 pKa = 3.06PVEE105 pKa = 3.92IARR108 pKa = 11.84ACLSLMITCKK118 pKa = 10.39KK119 pKa = 10.38SATNTEE125 pKa = 3.73RR126 pKa = 11.84MIFSIVQAPQILQSCKK142 pKa = 9.66VVANKK147 pKa = 9.64YY148 pKa = 10.07ASVSAVKK155 pKa = 10.03HH156 pKa = 4.56VKK158 pKa = 10.37APEE161 pKa = 4.02KK162 pKa = 10.96VPGDD166 pKa = 3.3GTLEE170 pKa = 4.02YY171 pKa = 10.52KK172 pKa = 10.99VNFVSLTVVPKK183 pKa = 10.08RR184 pKa = 11.84DD185 pKa = 3.37VYY187 pKa = 10.77RR188 pKa = 11.84IPSTALRR195 pKa = 11.84VSGPSLYY202 pKa = 10.95NLALNVIIAVEE213 pKa = 4.02VDD215 pKa = 3.78DD216 pKa = 5.54KK217 pKa = 11.99SPLTRR222 pKa = 11.84SLTRR226 pKa = 11.84TEE228 pKa = 4.74EE229 pKa = 4.4GFCANLFLHH238 pKa = 6.97IGLMSTVDD246 pKa = 3.43KK247 pKa = 10.63KK248 pKa = 11.2GKK250 pKa = 10.07KK251 pKa = 9.39ISFDD255 pKa = 3.06KK256 pKa = 11.14LEE258 pKa = 4.17RR259 pKa = 11.84KK260 pKa = 9.0IRR262 pKa = 11.84RR263 pKa = 11.84LDD265 pKa = 3.57LSVGLSDD272 pKa = 4.26VLGPSILIKK281 pKa = 10.78ARR283 pKa = 11.84GARR286 pKa = 11.84TRR288 pKa = 11.84LMSPFFSSSGTACYY302 pKa = 9.57PIANASPQVAKK313 pKa = 10.65ILWSQSASLRR323 pKa = 11.84SVKK326 pKa = 10.13IVIQAGTQKK335 pKa = 10.81AIAVTADD342 pKa = 3.47HH343 pKa = 6.65EE344 pKa = 4.93VMSTKK349 pKa = 10.43LEE351 pKa = 4.19KK352 pKa = 10.79NHH354 pKa = 6.76TISKK358 pKa = 9.32FNPFKK363 pKa = 10.85KK364 pKa = 10.49

Molecular weight: 39.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4625

364

2202

770.8

85.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.33 ± 0.755

1.795 ± 0.202

4.865 ± 0.285

4.995 ± 0.382

3.395 ± 0.598

5.946 ± 0.599

1.881 ± 0.441

6.746 ± 0.456

4.757 ± 0.427

10.551 ± 1.002

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.832 ± 0.252

4.67 ± 0.294

4.8 ± 0.566

4.173 ± 0.498

5.059 ± 0.531

9.016 ± 0.392

7.178 ± 0.726

6.119 ± 0.253

0.692 ± 0.131

3.2 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski