Amino acid dipepetide frequency for Streptomyces sp. PT12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.792AlaAla: 23.792 ± 0.203
1.164AlaCys: 1.164 ± 0.027
9.218AlaAsp: 9.218 ± 0.081
9.825AlaGlu: 9.825 ± 0.092
3.928AlaPhe: 3.928 ± 0.046
13.234AlaGly: 13.234 ± 0.095
3.267AlaHis: 3.267 ± 0.048
3.76AlaIle: 3.76 ± 0.045
1.913AlaLys: 1.913 ± 0.041
16.224AlaLeu: 16.224 ± 0.136
2.466AlaMet: 2.466 ± 0.035
1.745AlaAsn: 1.745 ± 0.035
8.26AlaPro: 8.26 ± 0.091
3.408AlaGln: 3.408 ± 0.048
11.942AlaArg: 11.942 ± 0.099
5.966AlaSer: 5.966 ± 0.056
7.466AlaThr: 7.466 ± 0.072
12.184AlaVal: 12.184 ± 0.094
2.085AlaTrp: 2.085 ± 0.036
2.48AlaTyr: 2.48 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.151CysAla: 1.151 ± 0.027
0.092CysCys: 0.092 ± 0.007
0.498CysAsp: 0.498 ± 0.016
0.381CysGlu: 0.381 ± 0.016
0.214CysPhe: 0.214 ± 0.011
0.954CysGly: 0.954 ± 0.023
0.223CysHis: 0.223 ± 0.011
0.112CysIle: 0.112 ± 0.008
0.09CysLys: 0.09 ± 0.008
0.691CysLeu: 0.691 ± 0.018
0.097CysMet: 0.097 ± 0.007
0.119CysAsn: 0.119 ± 0.008
0.469CysPro: 0.469 ± 0.015
0.18CysGln: 0.18 ± 0.009
0.653CysArg: 0.653 ± 0.02
0.42CysSer: 0.42 ± 0.018
0.402CysThr: 0.402 ± 0.017
0.648CysVal: 0.648 ± 0.019
0.145CysTrp: 0.145 ± 0.008
0.155CysTyr: 0.155 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.85AspAla: 8.85 ± 0.094
0.39AspCys: 0.39 ± 0.015
4.003AspAsp: 4.003 ± 0.065
4.647AspGlu: 4.647 ± 0.058
1.567AspPhe: 1.567 ± 0.027
6.562AspGly: 6.562 ± 0.074
1.522AspHis: 1.522 ± 0.028
2.023AspIle: 2.023 ± 0.035
0.674AspLys: 0.674 ± 0.023
6.251AspLeu: 6.251 ± 0.058
0.749AspMet: 0.749 ± 0.021
0.864AspAsn: 0.864 ± 0.026
4.88AspPro: 4.88 ± 0.054
1.498AspGln: 1.498 ± 0.027
5.023AspArg: 5.023 ± 0.053
2.243AspSer: 2.243 ± 0.033
2.926AspThr: 2.926 ± 0.039
4.691AspVal: 4.691 ± 0.055
0.989AspTrp: 0.989 ± 0.024
1.099AspTyr: 1.099 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
8.151GluAla: 8.151 ± 0.085
0.381GluCys: 0.381 ± 0.014
2.687GluAsp: 2.687 ± 0.04
3.683GluGlu: 3.683 ± 0.063
1.497GluPhe: 1.497 ± 0.032
5.341GluGly: 5.341 ± 0.056
1.542GluHis: 1.542 ± 0.027
2.164GluIle: 2.164 ± 0.034
0.937GluLys: 0.937 ± 0.028
7.114GluLeu: 7.114 ± 0.067
0.809GluMet: 0.809 ± 0.022
0.995GluAsn: 0.995 ± 0.022
3.958GluPro: 3.958 ± 0.056
1.88GluGln: 1.88 ± 0.03
7.072GluArg: 7.072 ± 0.074
2.701GluSer: 2.701 ± 0.04
2.999GluThr: 2.999 ± 0.039
4.595GluVal: 4.595 ± 0.054
0.812GluTrp: 0.812 ± 0.021
1.0GluTyr: 1.0 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.76PheAla: 3.76 ± 0.047
0.241PheCys: 0.241 ± 0.011
2.107PheAsp: 2.107 ± 0.035
1.399PheGlu: 1.399 ± 0.025
0.828PhePhe: 0.828 ± 0.026
2.979PheGly: 2.979 ± 0.043
0.673PheHis: 0.673 ± 0.02
0.716PheIle: 0.716 ± 0.021
0.327PheLys: 0.327 ± 0.014
2.497PheLeu: 2.497 ± 0.038
0.348PheMet: 0.348 ± 0.013
0.491PheAsn: 0.491 ± 0.019
1.405PhePro: 1.405 ± 0.024
0.658PheGln: 0.658 ± 0.019
1.831PheArg: 1.831 ± 0.031
1.42PheSer: 1.42 ± 0.031
1.951PheThr: 1.951 ± 0.031
2.049PheVal: 2.049 ± 0.038
0.393PheTrp: 0.393 ± 0.016
0.521PheTyr: 0.521 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
12.657GlyAla: 12.657 ± 0.098
0.842GlyCys: 0.842 ± 0.023
5.804GlyAsp: 5.804 ± 0.06
6.088GlyGlu: 6.088 ± 0.065
2.705GlyPhe: 2.705 ± 0.042
9.991GlyGly: 9.991 ± 0.111
2.494GlyHis: 2.494 ± 0.038
3.272GlyIle: 3.272 ± 0.043
1.583GlyLys: 1.583 ± 0.029
9.55GlyLeu: 9.55 ± 0.085
1.927GlyMet: 1.927 ± 0.033
1.622GlyAsn: 1.622 ± 0.035
5.641GlyPro: 5.641 ± 0.063
2.642GlyGln: 2.642 ± 0.048
8.359GlyArg: 8.359 ± 0.07
4.941GlySer: 4.941 ± 0.052
5.99GlyThr: 5.99 ± 0.064
7.768GlyVal: 7.768 ± 0.071
1.732GlyTrp: 1.732 ± 0.031
2.107GlyTyr: 2.107 ± 0.031
0.0GlyXaa: 0.0 ± 0.0
His
3.015HisAla: 3.015 ± 0.045
0.206HisCys: 0.206 ± 0.01
1.386HisAsp: 1.386 ± 0.028
1.269HisGlu: 1.269 ± 0.027
0.6HisPhe: 0.6 ± 0.019
2.51HisGly: 2.51 ± 0.038
0.758HisHis: 0.758 ± 0.021
0.708HisIle: 0.708 ± 0.019
0.253HisLys: 0.253 ± 0.011
2.591HisLeu: 2.591 ± 0.042
0.314HisMet: 0.314 ± 0.014
0.354HisAsn: 0.354 ± 0.014
2.0HisPro: 2.0 ± 0.035
0.628HisGln: 0.628 ± 0.02
2.398HisArg: 2.398 ± 0.04
0.965HisSer: 0.965 ± 0.023
1.267HisThr: 1.267 ± 0.028
1.733HisVal: 1.733 ± 0.027
0.372HisTrp: 0.372 ± 0.014
0.441HisTyr: 0.441 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.83IleAla: 4.83 ± 0.058
0.251IleCys: 0.251 ± 0.011
2.247IleAsp: 2.247 ± 0.036
2.063IleGlu: 2.063 ± 0.038
0.65IlePhe: 0.65 ± 0.021
3.446IleGly: 3.446 ± 0.042
0.627IleHis: 0.627 ± 0.016
0.819IleIle: 0.819 ± 0.025
0.51IleLys: 0.51 ± 0.015
2.183IleLeu: 2.183 ± 0.036
0.392IleMet: 0.392 ± 0.015
0.646IleAsn: 0.646 ± 0.018
1.772IlePro: 1.772 ± 0.031
0.641IleGln: 0.641 ± 0.019
2.278IleArg: 2.278 ± 0.034
1.592IleSer: 1.592 ± 0.031
2.07IleThr: 2.07 ± 0.037
2.468IleVal: 2.468 ± 0.043
0.36IleTrp: 0.36 ± 0.012
0.487IleTyr: 0.487 ± 0.019
0.0IleXaa: 0.0 ± 0.0
Lys
1.796LysAla: 1.796 ± 0.037
0.07LysCys: 0.07 ± 0.006
0.795LysAsp: 0.795 ± 0.025
0.848LysGlu: 0.848 ± 0.027
0.269LysPhe: 0.269 ± 0.013
1.158LysGly: 1.158 ± 0.029
0.317LysHis: 0.317 ± 0.014
0.53LysIle: 0.53 ± 0.018
0.399LysLys: 0.399 ± 0.023
1.28LysLeu: 1.28 ± 0.028
0.249LysMet: 0.249 ± 0.011
0.281LysAsn: 0.281 ± 0.014
0.897LysPro: 0.897 ± 0.023
0.388LysGln: 0.388 ± 0.016
1.229LysArg: 1.229 ± 0.029
0.746LysSer: 0.746 ± 0.022
0.81LysThr: 0.81 ± 0.022
1.15LysVal: 1.15 ± 0.028
0.162LysTrp: 0.162 ± 0.008
0.244LysTyr: 0.244 ± 0.012
0.0LysXaa: 0.0 ± 0.0
Leu
16.869LeuAla: 16.869 ± 0.137
0.823LeuCys: 0.823 ± 0.02
7.055LeuAsp: 7.055 ± 0.074
4.367LeuGlu: 4.367 ± 0.049
2.524LeuPhe: 2.524 ± 0.042
9.505LeuGly: 9.505 ± 0.067
2.3LeuHis: 2.3 ± 0.035
3.358LeuIle: 3.358 ± 0.048
1.278LeuLys: 1.278 ± 0.026
11.475LeuLeu: 11.475 ± 0.112
1.535LeuMet: 1.535 ± 0.034
1.51LeuAsn: 1.51 ± 0.028
6.9LeuPro: 6.9 ± 0.068
1.78LeuGln: 1.78 ± 0.034
9.416LeuArg: 9.416 ± 0.076
5.14LeuSer: 5.14 ± 0.05
7.153LeuThr: 7.153 ± 0.08
8.585LeuVal: 8.585 ± 0.076
1.35LeuTrp: 1.35 ± 0.03
1.696LeuTyr: 1.696 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.383MetAla: 2.383 ± 0.04
0.116MetCys: 0.116 ± 0.008
0.816MetAsp: 0.816 ± 0.02
0.701MetGlu: 0.701 ± 0.02
0.42MetPhe: 0.42 ± 0.016
1.32MetGly: 1.32 ± 0.028
0.302MetHis: 0.302 ± 0.012
0.584MetIle: 0.584 ± 0.02
0.232MetLys: 0.232 ± 0.011
1.466MetLeu: 1.466 ± 0.034
0.248MetMet: 0.248 ± 0.013
0.374MetAsn: 0.374 ± 0.014
1.035MetPro: 1.035 ± 0.022
0.303MetGln: 0.303 ± 0.01
1.465MetArg: 1.465 ± 0.026
1.189MetSer: 1.189 ± 0.024
1.382MetThr: 1.382 ± 0.028
1.174MetVal: 1.174 ± 0.025
0.174MetTrp: 0.174 ± 0.01
0.285MetTyr: 0.285 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.371AsnAla: 2.371 ± 0.037
0.118AsnCys: 0.118 ± 0.008
0.986AsnAsp: 0.986 ± 0.024
0.826AsnGlu: 0.826 ± 0.02
0.404AsnPhe: 0.404 ± 0.015
1.866AsnGly: 1.866 ± 0.038
0.349AsnHis: 0.349 ± 0.013
0.526AsnIle: 0.526 ± 0.018
0.224AsnLys: 0.224 ± 0.011
1.384AsnLeu: 1.384 ± 0.037
0.24AsnMet: 0.24 ± 0.011
0.358AsnAsn: 0.358 ± 0.016
1.152AsnPro: 1.152 ± 0.026
0.437AsnGln: 0.437 ± 0.016
1.098AsnArg: 1.098 ± 0.026
0.72AsnSer: 0.72 ± 0.021
0.848AsnThr: 0.848 ± 0.026
1.257AsnVal: 1.257 ± 0.026
0.228AsnTrp: 0.228 ± 0.011
0.355AsnTyr: 0.355 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.829ProAla: 8.829 ± 0.099
0.353ProCys: 0.353 ± 0.015
4.817ProAsp: 4.817 ± 0.054
5.096ProGlu: 5.096 ± 0.064
1.635ProPhe: 1.635 ± 0.029
7.56ProGly: 7.56 ± 0.074
1.524ProHis: 1.524 ± 0.031
1.31ProIle: 1.31 ± 0.03
0.755ProLys: 0.755 ± 0.023
5.853ProLeu: 5.853 ± 0.052
1.0ProMet: 1.0 ± 0.02
0.798ProAsn: 0.798 ± 0.02
4.505ProPro: 4.505 ± 0.079
1.457ProGln: 1.457 ± 0.033
4.91ProArg: 4.91 ± 0.061
3.117ProSer: 3.117 ± 0.05
3.313ProThr: 3.313 ± 0.044
5.462ProVal: 5.462 ± 0.062
1.042ProTrp: 1.042 ± 0.028
1.139ProTyr: 1.139 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.365GlnAla: 3.365 ± 0.051
0.146GlnCys: 0.146 ± 0.008
1.204GlnAsp: 1.204 ± 0.025
1.325GlnGlu: 1.325 ± 0.029
0.591GlnPhe: 0.591 ± 0.019
2.151GlnGly: 2.151 ± 0.035
0.553GlnHis: 0.553 ± 0.018
0.852GlnIle: 0.852 ± 0.022
0.314GlnLys: 0.314 ± 0.014
2.901GlnLeu: 2.901 ± 0.045
0.372GlnMet: 0.372 ± 0.014
0.385GlnAsn: 0.385 ± 0.015
1.575GlnPro: 1.575 ± 0.039
0.953GlnGln: 0.953 ± 0.03
2.631GlnArg: 2.631 ± 0.044
0.998GlnSer: 0.998 ± 0.025
1.093GlnThr: 1.093 ± 0.027
1.997GlnVal: 1.997 ± 0.035
0.378GlnTrp: 0.378 ± 0.012
0.469GlnTyr: 0.469 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
11.998ArgAla: 11.998 ± 0.098
0.689ArgCys: 0.689 ± 0.019
4.817ArgAsp: 4.817 ± 0.052
5.337ArgGlu: 5.337 ± 0.061
2.39ArgPhe: 2.39 ± 0.038
7.097ArgGly: 7.097 ± 0.069
2.364ArgHis: 2.364 ± 0.039
2.903ArgIle: 2.903 ± 0.041
1.217ArgLys: 1.217 ± 0.028
9.843ArgLeu: 9.843 ± 0.076
1.727ArgMet: 1.727 ± 0.036
1.312ArgAsn: 1.312 ± 0.025
5.489ArgPro: 5.489 ± 0.06
2.346ArgGln: 2.346 ± 0.034
9.002ArgArg: 9.002 ± 0.095
3.956ArgSer: 3.956 ± 0.048
4.764ArgThr: 4.764 ± 0.054
6.897ArgVal: 6.897 ± 0.067
1.552ArgTrp: 1.552 ± 0.03
1.838ArgTyr: 1.838 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.416SerAla: 6.416 ± 0.057
0.389SerCys: 0.389 ± 0.015
2.527SerAsp: 2.527 ± 0.035
2.399SerGlu: 2.399 ± 0.038
1.478SerPhe: 1.478 ± 0.026
5.747SerGly: 5.747 ± 0.059
1.015SerHis: 1.015 ± 0.023
1.278SerIle: 1.278 ± 0.024
0.595SerLys: 0.595 ± 0.02
4.78SerLeu: 4.78 ± 0.054
0.912SerMet: 0.912 ± 0.023
0.735SerAsn: 0.735 ± 0.02
3.288SerPro: 3.288 ± 0.047
1.169SerGln: 1.169 ± 0.025
3.603SerArg: 3.603 ± 0.044
2.538SerSer: 2.538 ± 0.042
2.602SerThr: 2.602 ± 0.038
3.888SerVal: 3.888 ± 0.048
0.927SerTrp: 0.927 ± 0.025
1.066SerTyr: 1.066 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
8.552ThrAla: 8.552 ± 0.079
0.37ThrCys: 0.37 ± 0.016
3.3ThrAsp: 3.3 ± 0.048
3.257ThrGlu: 3.257 ± 0.041
1.582ThrPhe: 1.582 ± 0.027
6.681ThrGly: 6.681 ± 0.072
1.174ThrHis: 1.174 ± 0.027
1.561ThrIle: 1.561 ± 0.038
0.701ThrLys: 0.701 ± 0.021
5.727ThrLeu: 5.727 ± 0.064
0.811ThrMet: 0.811 ± 0.022
0.823ThrAsn: 0.823 ± 0.021
4.052ThrPro: 4.052 ± 0.054
1.164ThrGln: 1.164 ± 0.023
4.175ThrArg: 4.175 ± 0.044
2.794ThrSer: 2.794 ± 0.039
3.495ThrThr: 3.495 ± 0.052
5.764ThrVal: 5.764 ± 0.053
0.865ThrTrp: 0.865 ± 0.022
1.15ThrTyr: 1.15 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
11.181ValAla: 11.181 ± 0.085
0.724ValCys: 0.724 ± 0.02
5.002ValAsp: 5.002 ± 0.056
4.871ValGlu: 4.871 ± 0.042
2.321ValPhe: 2.321 ± 0.041
6.521ValGly: 6.521 ± 0.063
1.919ValHis: 1.919 ± 0.035
2.951ValIle: 2.951 ± 0.05
1.158ValLys: 1.158 ± 0.028
9.032ValLeu: 9.032 ± 0.081
1.308ValMet: 1.308 ± 0.027
1.618ValAsn: 1.618 ± 0.033
5.246ValPro: 5.246 ± 0.059
1.634ValGln: 1.634 ± 0.027
7.105ValArg: 7.105 ± 0.061
4.187ValSer: 4.187 ± 0.047
5.485ValThr: 5.485 ± 0.06
7.538ValVal: 7.538 ± 0.078
1.08ValTrp: 1.08 ± 0.023
1.371ValTyr: 1.371 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.717TrpAla: 1.717 ± 0.03
0.155TrpCys: 0.155 ± 0.008
0.876TrpAsp: 0.876 ± 0.021
0.865TrpGlu: 0.865 ± 0.021
0.459TrpPhe: 0.459 ± 0.016
1.167TrpGly: 1.167 ± 0.027
0.399TrpHis: 0.399 ± 0.014
0.492TrpIle: 0.492 ± 0.018
0.236TrpLys: 0.236 ± 0.011
1.85TrpLeu: 1.85 ± 0.033
0.265TrpMet: 0.265 ± 0.011
0.391TrpAsn: 0.391 ± 0.016
0.868TrpPro: 0.868 ± 0.02
0.52TrpGln: 0.52 ± 0.018
1.548TrpArg: 1.548 ± 0.03
0.928TrpSer: 0.928 ± 0.022
0.958TrpThr: 0.958 ± 0.022
0.99TrpVal: 0.99 ± 0.022
0.324TrpTrp: 0.324 ± 0.013
0.336TrpTyr: 0.336 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.644TyrAla: 2.644 ± 0.037
0.176TyrCys: 0.176 ± 0.009
1.304TyrAsp: 1.304 ± 0.032
1.071TyrGlu: 1.071 ± 0.026
0.532TyrPhe: 0.532 ± 0.016
1.923TyrGly: 1.923 ± 0.034
0.446TyrHis: 0.446 ± 0.015
0.415TyrIle: 0.415 ± 0.016
0.219TyrLys: 0.219 ± 0.011
1.927TyrLeu: 1.927 ± 0.034
0.201TyrMet: 0.201 ± 0.01
0.324TyrAsn: 0.324 ± 0.013
1.086TyrPro: 1.086 ± 0.023
0.566TyrGln: 0.566 ± 0.018
1.802TyrArg: 1.802 ± 0.033
0.791TyrSer: 0.791 ± 0.02
1.022TyrThr: 1.022 ± 0.025
1.417TyrVal: 1.417 ± 0.028
0.375TyrTrp: 0.375 ± 0.015
0.395TyrTyr: 0.395 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5724 proteins (1969865 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski