Macaca mulatta papillomavirus 5
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386JT72|A0A386JT72_9PAPI Replication protein E1 OS=Macaca mulatta papillomavirus 5 OX=2364645 GN=E1 PE=3 SV=1
MM1 pKa = 7.34 QGEE4 pKa = 4.59 APTLKK9 pKa = 10.73 DD10 pKa = 2.75 IVLEE14 pKa = 4.05 FEE16 pKa = 4.3 EE17 pKa = 6.31 DD18 pKa = 3.29 ILPVNLLSEE27 pKa = 4.52 EE28 pKa = 4.41 DD29 pKa = 3.57 LSQEE33 pKa = 4.25 EE34 pKa = 4.37 EE35 pKa = 4.39 LEE37 pKa = 4.24 EE38 pKa = 4.67 PDD40 pKa = 3.32 RR41 pKa = 11.84 SPYY44 pKa = 10.88 SVVTHH49 pKa = 6.93 CDD51 pKa = 2.69 RR52 pKa = 11.84 CAKK55 pKa = 9.8 SLRR58 pKa = 11.84 IVVIATKK65 pKa = 10.63 DD66 pKa = 3.43 SVRR69 pKa = 11.84 SLEE72 pKa = 3.98 TLLFGSLSLLCPPCARR88 pKa = 11.84 SLRR91 pKa = 11.84 HH92 pKa = 5.8 GRR94 pKa = 3.52
Molecular weight: 10.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.664
IPC2_protein 4.685
IPC_protein 4.546
Toseland 4.406
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.635
Wikipedia 4.329
Rodwell 4.393
Grimsley 4.329
Solomon 4.482
Lehninger 4.431
Nozaki 4.596
DTASelect 4.698
Thurlkill 4.406
EMBOSS 4.355
Sillero 4.66
Patrickios 3.859
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.601
Protein with the highest isoelectric point:
>tr|A0A386JT15|A0A386JT15_9PAPI Protein E7 OS=Macaca mulatta papillomavirus 5 OX=2364645 GN=E7 PE=3 SV=1
MM1 pKa = 7.75 EE2 pKa = 5.66 PPSTLQYY9 pKa = 11.2 LCNLCNIRR17 pKa = 11.84 LDD19 pKa = 3.73 QLQIPCVFCKK29 pKa = 10.8 LNLSLFDD36 pKa = 3.58 LHH38 pKa = 6.8 HH39 pKa = 6.7 FSVLNLNLLLRR50 pKa = 11.84 DD51 pKa = 3.57 TVFYY55 pKa = 10.39 GCCRR59 pKa = 11.84 KK60 pKa = 9.81 CLHH63 pKa = 6.76 LSAAYY68 pKa = 9.44 EE69 pKa = 4.2 FYY71 pKa = 10.72 KK72 pKa = 10.71 YY73 pKa = 8.66 YY74 pKa = 10.35 QCSADD79 pKa = 3.15 IEE81 pKa = 4.85 YY82 pKa = 8.69 ITVLSSEE89 pKa = 4.38 TLGRR93 pKa = 11.84 LQIRR97 pKa = 11.84 CEE99 pKa = 3.95 TCMKK103 pKa = 10.49 RR104 pKa = 11.84 LTVTEE109 pKa = 4.76 KK110 pKa = 10.89 YY111 pKa = 10.74 DD112 pKa = 3.81 CVSNKK117 pKa = 10.0 DD118 pKa = 3.02 KK119 pKa = 11.12 CHH121 pKa = 6.01 LVRR124 pKa = 11.84 GIWRR128 pKa = 11.84 APCRR132 pKa = 11.84 LCRR135 pKa = 11.84 KK136 pKa = 9.32 KK137 pKa = 10.81
Molecular weight: 16.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.748
IPC2_protein 7.732
IPC_protein 7.527
Toseland 6.956
ProMoST 8.229
Dawson 8.214
Bjellqvist 8.756
Wikipedia 8.112
Rodwell 8.244
Grimsley 6.942
Solomon 8.273
Lehninger 8.302
Nozaki 9.077
DTASelect 8.375
Thurlkill 8.419
EMBOSS 8.434
Sillero 8.814
Patrickios 1.074
IPC_peptide 8.273
IPC2_peptide 8.229
IPC2.peptide.svr19 8.204
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2425
94
610
346.4
38.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.608 ± 0.649
2.062 ± 0.888
6.557 ± 0.372
6.639 ± 0.949
4.495 ± 0.48
6.392 ± 0.907
1.897 ± 0.309
5.113 ± 0.494
5.278 ± 0.775
9.155 ± 1.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.526 ± 0.276
4.412 ± 0.467
6.103 ± 1.034
3.794 ± 0.367
5.938 ± 0.866
8.33 ± 0.65
6.144 ± 0.913
5.979 ± 0.362
1.155 ± 0.272
3.423 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here