Stenotrophomonas maltophilia (strain K279a)
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4365 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2FSK9|B2FSK9_STRMK Putative ABC transporter ATP-binding protein OS=Stenotrophomonas maltophilia (strain K279a) OX=522373 GN=Smlt1185 PE=4 SV=1
MM1 pKa = 7.14 NLVDD5 pKa = 4.97 RR6 pKa = 11.84 FLSGLIPRR14 pKa = 11.84 LPADD18 pKa = 5.3 DD19 pKa = 4.44 APQWAHH25 pKa = 4.92 VQGASAEE32 pKa = 4.03 DD33 pKa = 3.74 LQRR36 pKa = 11.84 LRR38 pKa = 11.84 MQWPQVPDD46 pKa = 3.56 SLVEE50 pKa = 3.79 LLSRR54 pKa = 11.84 VDD56 pKa = 3.29 GTHH59 pKa = 5.14 FRR61 pKa = 11.84 EE62 pKa = 4.32 YY63 pKa = 10.02 PGGGVCVLMLGSDD76 pKa = 4.07 VEE78 pKa = 4.68 DD79 pKa = 3.68 GGYY82 pKa = 9.77 PYY84 pKa = 10.97 YY85 pKa = 10.55 LRR87 pKa = 11.84 SVAQIFEE94 pKa = 4.72 DD95 pKa = 3.61 QQQWDD100 pKa = 3.52 DD101 pKa = 4.29 SIRR104 pKa = 11.84 SIYY107 pKa = 9.47 EE108 pKa = 3.32 EE109 pKa = 4.08 WLDD112 pKa = 4.85 DD113 pKa = 3.82 EE114 pKa = 5.18 PEE116 pKa = 4.01 ILGEE120 pKa = 5.11 GIDD123 pKa = 5.01 ADD125 pKa = 3.93 LPMNRR130 pKa = 11.84 RR131 pKa = 11.84 LCFSHH136 pKa = 6.69 CMNNGGTSMLYY147 pKa = 10.57 LDD149 pKa = 5.5 FNPAPGGTVGQVVRR163 pKa = 11.84 YY164 pKa = 9.01 LHH166 pKa = 7.24 DD167 pKa = 5.09 PDD169 pKa = 4.6 SYY171 pKa = 12.0 AVIAPSFDD179 pKa = 4.49 AYY181 pKa = 10.67 LQQLIDD187 pKa = 3.61 GGYY190 pKa = 10.42 AFIDD194 pKa = 3.85 EE195 pKa = 4.52 EE196 pKa = 4.34 QQ197 pKa = 3.2
Molecular weight: 22.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.986
IPC_protein 3.973
Toseland 3.757
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.808
Grimsley 3.668
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.317
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.101
Patrickios 1.189
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.973
Protein with the highest isoelectric point:
>sp|B2FN90|IF2_STRMK Translation initiation factor IF-2 OS=Stenotrophomonas maltophilia (strain K279a) OX=522373 GN=infB PE=3 SV=1
MM1 pKa = 7.64 WPRR4 pKa = 11.84 AFAGTVAGFLLAAAATGLLAWVPPGPWQRR33 pKa = 11.84 ALVPTLIAFIPLWMLAALWAFSFRR57 pKa = 11.84 SALRR61 pKa = 11.84 AWLVLGGSAAAGFAMLGLLRR81 pKa = 11.84 LTGAVQQ87 pKa = 3.12
Molecular weight: 9.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4365
0
4365
1427787
38
4127
327.1
35.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.875 ± 0.054
0.823 ± 0.013
5.675 ± 0.031
5.065 ± 0.036
3.268 ± 0.027
8.626 ± 0.062
2.245 ± 0.021
4.015 ± 0.028
2.686 ± 0.032
11.034 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.203 ± 0.018
2.638 ± 0.037
5.335 ± 0.038
4.407 ± 0.029
7.475 ± 0.042
5.434 ± 0.03
4.923 ± 0.039
7.371 ± 0.033
1.609 ± 0.018
2.294 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here