Candidatus Halobonum tyrrellensis G22

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Candidatus Halobonum; Candidatus Halobonum tyrrellensis

Average proteome isoelectric point is 4.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4HBA0|V4HBA0_9EURY Uncharacterized protein OS=Candidatus Halobonum tyrrellensis G22 OX=1324957 GN=K933_14468 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.27YY4 pKa = 11.4AKK6 pKa = 10.71DD7 pKa = 3.81VLVSADD13 pKa = 3.23WVEE16 pKa = 3.85EE17 pKa = 3.91HH18 pKa = 7.19LDD20 pKa = 3.7EE21 pKa = 5.3FQSDD25 pKa = 3.57DD26 pKa = 3.48DD27 pKa = 4.17EE28 pKa = 4.93YY29 pKa = 11.73RR30 pKa = 11.84LVEE33 pKa = 3.98VDD35 pKa = 4.17VDD37 pKa = 3.84TEE39 pKa = 4.62AYY41 pKa = 10.29DD42 pKa = 3.54SGHH45 pKa = 6.64APGAIGFNWEE55 pKa = 4.26TQLQDD60 pKa = 3.03QTRR63 pKa = 11.84RR64 pKa = 11.84DD65 pKa = 3.71LLTTEE70 pKa = 5.1DD71 pKa = 4.87FEE73 pKa = 5.96DD74 pKa = 4.18LNASHH79 pKa = 7.27GISNDD84 pKa = 3.2STVVIYY90 pKa = 10.83GDD92 pKa = 3.53SSNWFAAYY100 pKa = 8.43TYY102 pKa = 8.79WQYY105 pKa = 11.8KK106 pKa = 9.41YY107 pKa = 10.41YY108 pKa = 10.98GHH110 pKa = 7.78DD111 pKa = 3.99DD112 pKa = 3.38VRR114 pKa = 11.84LMDD117 pKa = 4.23GGRR120 pKa = 11.84EE121 pKa = 3.84YY122 pKa = 10.27WVDD125 pKa = 2.94NDD127 pKa = 3.9YY128 pKa = 8.15PTTDD132 pKa = 4.03EE133 pKa = 4.57EE134 pKa = 4.64PSFPEE139 pKa = 3.89TEE141 pKa = 4.19YY142 pKa = 11.13EE143 pKa = 3.82AAEE146 pKa = 4.14PDD148 pKa = 3.45EE149 pKa = 4.97SIRR152 pKa = 11.84AYY154 pKa = 10.55RR155 pKa = 11.84EE156 pKa = 3.7QVDD159 pKa = 3.44AAIDD163 pKa = 3.6AGVPLVDD170 pKa = 3.35VRR172 pKa = 11.84SPEE175 pKa = 3.94EE176 pKa = 3.64FRR178 pKa = 11.84GEE180 pKa = 3.65ILAPPGLQEE189 pKa = 3.56TAQRR193 pKa = 11.84GGHH196 pKa = 6.15IPGASNISWASTVNEE211 pKa = 4.56DD212 pKa = 4.16GTFKK216 pKa = 10.42TADD219 pKa = 3.71EE220 pKa = 4.16LQEE223 pKa = 4.33LYY225 pKa = 10.88EE226 pKa = 4.19GVIDD230 pKa = 5.16AGDD233 pKa = 3.91DD234 pKa = 3.82EE235 pKa = 4.64IVAYY239 pKa = 10.34CRR241 pKa = 11.84IGEE244 pKa = 4.16RR245 pKa = 11.84SSIAWFALHH254 pKa = 6.86EE255 pKa = 4.66LLGQDD260 pKa = 2.8QTVNYY265 pKa = 9.6DD266 pKa = 3.59GSWTEE271 pKa = 3.62WGNLVGAPVEE281 pKa = 4.36TGDD284 pKa = 4.99AEE286 pKa = 4.12

Molecular weight: 32.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4HGH7|V4HGH7_9EURY Putative hydrolase OS=Candidatus Halobonum tyrrellensis G22 OX=1324957 GN=K933_04351 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 7.95RR3 pKa = 11.84TPHH6 pKa = 6.98DD7 pKa = 3.57EE8 pKa = 4.04HH9 pKa = 8.79DD10 pKa = 3.98GMKK13 pKa = 9.93VWLAADD19 pKa = 3.59EE20 pKa = 4.61ADD22 pKa = 4.95DD23 pKa = 4.01YY24 pKa = 11.77LALVEE29 pKa = 4.52DD30 pKa = 4.1TTHH33 pKa = 7.74RR34 pKa = 11.84IALSLGLRR42 pKa = 11.84CGLRR46 pKa = 11.84SAEE49 pKa = 4.12IVQTTPGDD57 pKa = 3.86LADD60 pKa = 4.75GPAGTMLRR68 pKa = 11.84VPDD71 pKa = 4.13GKK73 pKa = 10.49GGKK76 pKa = 8.93YY77 pKa = 9.52RR78 pKa = 11.84EE79 pKa = 4.34TPVPPEE85 pKa = 4.37LKK87 pKa = 8.34TTIQTVDD94 pKa = 3.11NVRR97 pKa = 11.84AEE99 pKa = 4.3PSSSPLVDD107 pKa = 3.18ASTRR111 pKa = 11.84TLRR114 pKa = 11.84RR115 pKa = 11.84WVRR118 pKa = 11.84KK119 pKa = 9.96SPLKK123 pKa = 10.13RR124 pKa = 11.84PKK126 pKa = 10.45SRR128 pKa = 11.84ATTAGGTSRR137 pKa = 11.84RR138 pKa = 11.84TTSGGHH144 pKa = 5.77GRR146 pKa = 11.84RR147 pKa = 11.84CSPEE151 pKa = 3.42PRR153 pKa = 11.84RR154 pKa = 11.84LTRR157 pKa = 11.84CSSVSGAGGRR167 pKa = 11.84TGNALRR173 pKa = 11.84ALPWRR178 pKa = 11.84LLSGGGTMRR187 pKa = 11.84GAA189 pKa = 3.8

Molecular weight: 20.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3445

0

3445

1030664

22

2237

299.2

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.37 ± 0.08

0.679 ± 0.015

8.921 ± 0.062

7.919 ± 0.06

3.274 ± 0.028

9.422 ± 0.047

1.857 ± 0.021

2.664 ± 0.038

1.366 ± 0.025

8.729 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.624 ± 0.019

2.046 ± 0.024

4.9 ± 0.026

1.885 ± 0.023

7.231 ± 0.05

5.262 ± 0.031

6.237 ± 0.037

9.789 ± 0.047

1.149 ± 0.018

2.676 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski