Gemmataceae bacterium
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6468 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V0I0X3|A0A4V0I0X3_9BACT Uncharacterized protein OS=Gemmataceae bacterium OX=2052164 GN=GCJUQL4_07910 PE=4 SV=1
MM1 pKa = 7.52 PRR3 pKa = 11.84 TRR5 pKa = 11.84 RR6 pKa = 11.84 DD7 pKa = 3.4 EE8 pKa = 4.36 PDD10 pKa = 3.27 EE11 pKa = 5.93 DD12 pKa = 4.42 EE13 pKa = 5.15 EE14 pKa = 5.09 DD15 pKa = 3.46 WDD17 pKa = 6.03 DD18 pKa = 4.62 GDD20 pKa = 6.08 DD21 pKa = 4.26 YY22 pKa = 11.87 DD23 pKa = 5.79 PEE25 pKa = 5.52 DD26 pKa = 4.73 PEE28 pKa = 4.51 TYY30 pKa = 9.9 PAGLYY35 pKa = 10.68 DD36 pKa = 5.08 DD37 pKa = 6.08 DD38 pKa = 5.98 GPATVPCPHH47 pKa = 6.71 CRR49 pKa = 11.84 AEE51 pKa = 3.88 ILEE54 pKa = 4.31 DD55 pKa = 3.67 SEE57 pKa = 4.36 RR58 pKa = 11.84 CPRR61 pKa = 11.84 CGEE64 pKa = 4.02 YY65 pKa = 10.66 LSAEE69 pKa = 4.06 DD70 pKa = 5.41 APRR73 pKa = 11.84 QLSGTAWVVLVLMLVAILLTVLGIGG98 pKa = 3.81
Molecular weight: 10.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A4V6M2D1|A0A4V6M2D1_9BACT Uncharacterized protein OS=Gemmataceae bacterium OX=2052164 GN=GCJUQL4_57730 PE=4 SV=1
MM1 pKa = 7.36 NPHH4 pKa = 7.03 RR5 pKa = 11.84 LPWTALAARR14 pKa = 11.84 FRR16 pKa = 11.84 AAAPRR21 pKa = 11.84 SAAPRR26 pKa = 11.84 AARR29 pKa = 11.84 APRR32 pKa = 11.84 AAGGHH37 pKa = 6.16 PGRR40 pKa = 11.84 RR41 pKa = 11.84 PLNQLFAPAAAEE53 pKa = 4.15 PPAAPATSATGPTASTVAA71 pKa = 3.68
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.774
IPC_protein 12.384
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.047
Grimsley 12.588
Solomon 13.042
Lehninger 12.939
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.798
IPC_peptide 13.042
IPC2_peptide 12.032
IPC2.peptide.svr19 9.183
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6468
0
6468
2271994
29
3244
351.3
37.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.887 ± 0.047
1.046 ± 0.012
5.86 ± 0.02
5.465 ± 0.025
3.541 ± 0.018
8.744 ± 0.032
2.052 ± 0.015
3.112 ± 0.02
4.089 ± 0.042
9.778 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.698 ± 0.014
2.473 ± 0.019
6.7 ± 0.037
2.733 ± 0.017
7.32 ± 0.034
4.443 ± 0.022
5.725 ± 0.035
8.583 ± 0.028
1.512 ± 0.012
2.235 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here