Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella pneumoniae; Klebsiella pneumoniae subsp. pneumoniae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5728 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3GVY0|A0A0H3GVY0_KLEPH Uncharacterized protein OS=Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) OX=1125630 GN=KPHS_p101410 PE=4 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.5CSADD7 pKa = 3.62EE8 pKa = 4.37TPVCCCMDD16 pKa = 2.94VGTIVDD22 pKa = 4.05NTDD25 pKa = 3.01CTASYY30 pKa = 11.33SRR32 pKa = 11.84VFANRR37 pKa = 11.84AEE39 pKa = 4.22AEE41 pKa = 4.04QTLAALSEE49 pKa = 4.16KK50 pKa = 10.29ARR52 pKa = 11.84NVEE55 pKa = 4.85SEE57 pKa = 4.21PCQINPTFTDD67 pKa = 2.69VDD69 pKa = 3.66GGVQLDD75 pKa = 3.18IDD77 pKa = 4.1FVFSCEE83 pKa = 3.94AEE85 pKa = 4.07SLIFQLGLRR94 pKa = 4.32

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3GZ75|A0A0H3GZ75_KLEPH Cytochrome c-type biogenesis protein CcmI OS=Klebsiella pneumoniae subsp. pneumoniae (strain HS11286) OX=1125630 GN=KPHS_30510 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5728

0

5728

1646261

37

3163

287.4

31.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.437 ± 0.042

1.09 ± 0.012

5.114 ± 0.027

5.48 ± 0.036

3.858 ± 0.023

7.548 ± 0.032

2.261 ± 0.017

5.597 ± 0.027

3.933 ± 0.03

11.007 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.019

3.442 ± 0.023

4.611 ± 0.023

4.51 ± 0.026

5.984 ± 0.033

5.794 ± 0.027

5.214 ± 0.024

7.035 ± 0.028

1.584 ± 0.015

2.747 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski