Rhizoctonia solani

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Cantharellales; Ceratobasidiaceae; Rhizoctonia

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11878 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K6FSL0|A0A0K6FSL0_9AGAM Retrotransposon-derived protein PEG10 OS=Rhizoctonia solani OX=456999 GN=RSOLAG22IIIB_08230 PE=4 SV=1
MM1 pKa = 7.58SSILAIALFAQVIAANPIYY20 pKa = 10.17RR21 pKa = 11.84RR22 pKa = 11.84QDD24 pKa = 2.94NGTTTITEE32 pKa = 4.12VTEE35 pKa = 4.42TPTATSTLSANATSITTDD53 pKa = 4.56PITFTVPTDD62 pKa = 3.35TTAIPITFSTAITSVAPSPTEE83 pKa = 3.99GSDD86 pKa = 3.75DD87 pKa = 3.46GSYY90 pKa = 9.48WYY92 pKa = 10.61CSFPGSEE99 pKa = 4.11ATATATATATAITTDD114 pKa = 3.47PFSTYY119 pKa = 9.48TSIDD123 pKa = 3.73DD124 pKa = 3.77TTVIADD130 pKa = 3.54PTFTDD135 pKa = 3.29SATEE139 pKa = 3.77ILTPTSTLTVPDD151 pKa = 3.58VTEE154 pKa = 4.15TATGTLSDD162 pKa = 4.33TEE164 pKa = 4.81LPTATEE170 pKa = 4.21TATDD174 pKa = 3.75TDD176 pKa = 3.79VTTATEE182 pKa = 4.35STTDD186 pKa = 3.12TDD188 pKa = 4.72IITATITAVTPTATDD203 pKa = 3.25TAIITDD209 pKa = 3.68VTSVSEE215 pKa = 4.11TDD217 pKa = 3.32TFIDD221 pKa = 3.65TFTDD225 pKa = 3.37TFTDD229 pKa = 3.59TFTDD233 pKa = 3.01TSTFVEE239 pKa = 4.81ATATTGPIVTITSVPSRR256 pKa = 11.84VQTYY260 pKa = 8.37TIVGPSTTAVIKK272 pKa = 9.85CIRR275 pKa = 11.84IRR277 pKa = 11.84GGPGRR282 pKa = 11.84PHH284 pKa = 6.56SPYY287 pKa = 9.67PHH289 pKa = 6.8PHH291 pKa = 7.49PYY293 pKa = 9.49PHH295 pKa = 7.56PNPPHH300 pKa = 6.6HH301 pKa = 7.21SSGSPQTFSGFPTATTDD318 pKa = 3.6VLGPTATEE326 pKa = 4.02TLPATSIVSFYY337 pKa = 10.45TAVPTTTFGVVGG349 pKa = 3.93

Molecular weight: 36.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K6FYK2|A0A0K6FYK2_9AGAM RRM domain-containing protein OS=Rhizoctonia solani OX=456999 GN=RSOLAG22IIIB_04502 PE=4 SV=1
MM1 pKa = 7.04QPKK4 pKa = 9.76LLSVLITFFGLATASPIMQGRR25 pKa = 11.84AVPSAKK31 pKa = 9.71PHH33 pKa = 6.36PPPSVTASLPPHH45 pKa = 6.87PSLSAFPSFSPLPPPHH61 pKa = 6.77HH62 pKa = 6.73SFVGRR67 pKa = 11.84QAPSSFPPPPPPPSSLPPRR86 pKa = 11.84PTFSPRR92 pKa = 11.84PPPPPPSLAGRR103 pKa = 11.84QAPSSLRR110 pKa = 11.84PPPPPPSSLPPRR122 pKa = 11.84PTFSPRR128 pKa = 11.84PPPPAPLAGRR138 pKa = 11.84QAPSSFPHH146 pKa = 6.77PPPPPSSLPPRR157 pKa = 11.84PTFSPLPPPPPPSLAARR174 pKa = 11.84QAPSSFPPPPPPPSSLPPRR193 pKa = 11.84PTFSPRR199 pKa = 11.84PPPPPPSLAGRR210 pKa = 11.84QAPSSRR216 pKa = 11.84PPPPPPSSLPPRR228 pKa = 11.84PTLSLPPPPPSPTASAA244 pKa = 3.77

Molecular weight: 25.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11878

0

11878

6275154

66

5018

528.3

57.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.418 ± 0.023

1.183 ± 0.008

5.396 ± 0.016

5.79 ± 0.021

3.432 ± 0.011

6.67 ± 0.022

2.448 ± 0.01

4.938 ± 0.014

4.458 ± 0.019

8.801 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.008

3.602 ± 0.012

6.528 ± 0.034

3.749 ± 0.014

5.996 ± 0.02

8.387 ± 0.025

6.145 ± 0.017

6.17 ± 0.017

1.45 ± 0.008

2.709 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski