Kandleria vitulina DSM 20405
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2043 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2HEP7|A0A0R2HEP7_9FIRM Aminopyrimidine aminohydrolase OS=Kandleria vitulina DSM 20405 OX=1410657 GN=IV49_GL000140 PE=3 SV=1
MM1 pKa = 7.99 KK2 pKa = 10.14 DD3 pKa = 3.24 NKK5 pKa = 9.22 ITVYY9 pKa = 10.51 DD10 pKa = 3.39 QSGEE14 pKa = 4.07 KK15 pKa = 10.22 KK16 pKa = 10.05 EE17 pKa = 4.03 FEE19 pKa = 4.15 ILFTYY24 pKa = 10.68 DD25 pKa = 4.77 DD26 pKa = 4.58 EE27 pKa = 6.94 DD28 pKa = 4.3 SSSHH32 pKa = 4.49 YY33 pKa = 10.6 VFYY36 pKa = 11.03 YY37 pKa = 10.71 DD38 pKa = 5.25 EE39 pKa = 4.6 NDD41 pKa = 3.39 EE42 pKa = 4.26 EE43 pKa = 4.55 QVFVSKK49 pKa = 10.95 YY50 pKa = 10.14 DD51 pKa = 3.89 EE52 pKa = 4.68 EE53 pKa = 4.53 GHH55 pKa = 7.07 LYY57 pKa = 9.98 PIEE60 pKa = 5.36 DD61 pKa = 3.61 PKK63 pKa = 11.31 EE64 pKa = 3.7 WDD66 pKa = 3.26 KK67 pKa = 11.71 TEE69 pKa = 4.9 EE70 pKa = 4.06 ILDD73 pKa = 3.86 SYY75 pKa = 10.45 LANDD79 pKa = 3.65 AVDD82 pKa = 4.55 GEE84 pKa = 4.87 SCGGEE89 pKa = 3.96 DD90 pKa = 5.26 CEE92 pKa = 5.05
Molecular weight: 10.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.567
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.656
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.037
Thurlkill 3.617
EMBOSS 3.668
Sillero 3.884
Patrickios 0.693
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A0R2HDQ6|A0A0R2HDQ6_9FIRM PfkB domain-containing protein OS=Kandleria vitulina DSM 20405 OX=1410657 GN=IV49_GL001781 PE=4 SV=1
MM1 pKa = 7.13 NMKK4 pKa = 9.17 RR5 pKa = 11.84 TYY7 pKa = 10.32 QPNKK11 pKa = 8.62 RR12 pKa = 11.84 KK13 pKa = 9.56 RR14 pKa = 11.84 AKK16 pKa = 8.76 THH18 pKa = 5.23 GFRR21 pKa = 11.84 ARR23 pKa = 11.84 MATVGGRR30 pKa = 11.84 KK31 pKa = 9.34 VIARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.99 KK39 pKa = 10.04 GRR41 pKa = 11.84 KK42 pKa = 8.66 VLSAA46 pKa = 4.05
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2043
0
2043
621057
37
2775
304.0
34.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.258 ± 0.06
1.219 ± 0.02
6.119 ± 0.048
7.048 ± 0.069
4.303 ± 0.046
6.193 ± 0.057
2.431 ± 0.031
8.179 ± 0.054
8.232 ± 0.061
9.09 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.096 ± 0.023
4.978 ± 0.051
2.85 ± 0.028
2.823 ± 0.023
3.794 ± 0.042
6.06 ± 0.045
5.222 ± 0.044
6.697 ± 0.04
0.693 ± 0.019
4.66 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here