Pseudoalteromonas ulvae
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3964 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A244CKL1|A0A244CKL1_9GAMM Sigma-54-dependent Fis family transcriptional regulator OS=Pseudoalteromonas ulvae OX=107327 GN=B1199_20620 PE=4 SV=1
MM1 pKa = 7.17 SHH3 pKa = 6.0 NHH5 pKa = 5.67 TFSEE9 pKa = 4.14 QDD11 pKa = 3.0 WPFDD15 pKa = 3.97 CPVEE19 pKa = 4.2 TLVVTTKK26 pKa = 10.76 LVYY29 pKa = 10.4 DD30 pKa = 3.59 KK31 pKa = 11.09 SEE33 pKa = 4.92 PIVQVIHH40 pKa = 6.71 YY41 pKa = 9.31 EE42 pKa = 4.02 DD43 pKa = 5.31 GEE45 pKa = 4.37 WQFMCNTTDD54 pKa = 4.61 DD55 pKa = 4.56 PDD57 pKa = 5.74 DD58 pKa = 5.54 GIVVCMGCFFQKK70 pKa = 10.33 FPEE73 pKa = 4.39 VAQLADD79 pKa = 3.85 LPLGFDD85 pKa = 4.86 AYY87 pKa = 10.85 RR88 pKa = 11.84 DD89 pKa = 4.62 DD90 pKa = 5.98 INQPWDD96 pKa = 3.25 IEE98 pKa = 4.19 KK99 pKa = 11.19 LEE101 pKa = 4.31 DD102 pKa = 3.27
Molecular weight: 11.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.592
ProMoST 3.923
Dawson 3.808
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.935
Patrickios 0.947
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.853
Protein with the highest isoelectric point:
>tr|A0A244CKI8|A0A244CKI8_9GAMM Uncharacterized protein OS=Pseudoalteromonas ulvae OX=107327 GN=B1199_20610 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.42 RR12 pKa = 11.84 KK13 pKa = 9.36 RR14 pKa = 11.84 SHH16 pKa = 6.15 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATTNGRR28 pKa = 11.84 KK29 pKa = 9.73 LIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3964
0
3964
1332564
37
2353
336.2
37.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.691 ± 0.039
1.036 ± 0.014
5.491 ± 0.035
5.873 ± 0.033
4.425 ± 0.026
6.256 ± 0.035
2.43 ± 0.023
6.522 ± 0.03
5.315 ± 0.029
10.683 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.334 ± 0.017
4.578 ± 0.03
3.739 ± 0.022
5.436 ± 0.041
4.027 ± 0.024
6.771 ± 0.033
5.472 ± 0.028
6.612 ± 0.034
1.16 ± 0.014
3.148 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here