Thalassiosira oceanica (Marine diatom)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales; Thalassiosiraceae; Thalassiosira

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0SFV4|K0SFV4_THAOC Uncharacterized protein OS=Thalassiosira oceanica OX=159749 GN=THAOC_19921 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.81RR3 pKa = 11.84MFDD6 pKa = 3.19GATAFDD12 pKa = 3.5QDD14 pKa = 3.92LCHH17 pKa = 7.1FGDD20 pKa = 4.38NFNQYY25 pKa = 10.51KK26 pKa = 9.63IVYY29 pKa = 9.17NMFVGSGCANKK40 pKa = 10.05SDD42 pKa = 4.1PTSEE46 pKa = 4.04TGPWCAVTNCLYY58 pKa = 10.74RR59 pKa = 11.84FTINTEE65 pKa = 3.44LRR67 pKa = 11.84DD68 pKa = 4.18AIKK71 pKa = 10.32EE72 pKa = 3.93YY73 pKa = 10.75LGQDD77 pKa = 3.52CPSDD81 pKa = 4.02LNCQARR87 pKa = 11.84SNYY90 pKa = 9.6GGAIGEE96 pKa = 4.22WDD98 pKa = 3.82VSSVDD103 pKa = 3.32DD104 pKa = 4.34FSRR107 pKa = 11.84LFVDD111 pKa = 5.92DD112 pKa = 4.72SDD114 pKa = 5.65DD115 pKa = 5.11PIDD118 pKa = 4.08GAATFNEE125 pKa = 4.23PLNWDD130 pKa = 3.65TGSATMMLYY139 pKa = 9.35MFKK142 pKa = 10.67DD143 pKa = 3.35AAAFNQPLSTFDD155 pKa = 3.26TSKK158 pKa = 9.95VNNMFGMFSGAAAFNRR174 pKa = 11.84DD175 pKa = 3.07LTAFDD180 pKa = 3.53TSEE183 pKa = 4.29VEE185 pKa = 4.27DD186 pKa = 4.4VSVLLCC192 pKa = 4.57

Molecular weight: 21.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0R635|K0R635_THAOC Uncharacterized protein (Fragment) OS=Thalassiosira oceanica OX=159749 GN=THAOC_33006 PE=4 SV=1
LL1 pKa = 7.23RR2 pKa = 11.84LSIKK6 pKa = 9.43GWLRR10 pKa = 11.84VRR12 pKa = 11.84RR13 pKa = 11.84LRR15 pKa = 11.84VRR17 pKa = 11.84QLRR20 pKa = 11.84VRR22 pKa = 11.84RR23 pKa = 11.84LSVRR27 pKa = 11.84SS28 pKa = 3.53

Molecular weight: 3.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34431

0

34431

12213943

10

6711

354.7

38.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.851 ± 0.015

1.856 ± 0.007

6.287 ± 0.011

6.526 ± 0.013

3.141 ± 0.008

8.08 ± 0.017

2.333 ± 0.007

3.896 ± 0.008

4.641 ± 0.014

8.354 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.006

3.446 ± 0.009

5.755 ± 0.015

3.469 ± 0.009

7.649 ± 0.019

8.574 ± 0.017

5.34 ± 0.011

6.238 ± 0.01

1.172 ± 0.005

2.284 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski