Acholeplasma axanthum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Acholeplasma

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449BBV4|A0A449BBV4_ACHAX Repeat OS=Acholeplasma axanthum OX=29552 GN=NCTC10138_00287 PE=4 SV=1
MM1 pKa = 7.74EE2 pKa = 5.53KK3 pKa = 10.66FSLLQYY9 pKa = 8.01TKK11 pKa = 10.78KK12 pKa = 10.45IIEE15 pKa = 4.33KK16 pKa = 10.17LDD18 pKa = 3.35NEE20 pKa = 4.44NLYY23 pKa = 8.48ITHH26 pKa = 6.97INIAYY31 pKa = 9.51KK32 pKa = 10.44FISYY36 pKa = 10.33YY37 pKa = 10.28EE38 pKa = 3.99NNIGNIDD45 pKa = 4.42SITEE49 pKa = 3.74EE50 pKa = 4.67HH51 pKa = 6.96FDD53 pKa = 3.59NMISAVYY60 pKa = 9.6EE61 pKa = 3.98RR62 pKa = 11.84YY63 pKa = 9.61IEE65 pKa = 4.35SDD67 pKa = 3.2SADD70 pKa = 3.31ILDD73 pKa = 4.78EE74 pKa = 4.74IIEE77 pKa = 4.56NILNEE82 pKa = 4.88DD83 pKa = 3.7SLVWW87 pKa = 3.64

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449BBX2|A0A449BBX2_ACHAX Transport permease protein OS=Acholeplasma axanthum OX=29552 GN=NCTC10138_00289 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.4RR12 pKa = 11.84SKK14 pKa = 8.49THH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTVGGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.37KK37 pKa = 10.24GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1645

0

1645

560043

33

6240

340.5

38.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.95 ± 0.058

0.416 ± 0.016

5.72 ± 0.041

7.112 ± 0.066

4.925 ± 0.049

5.587 ± 0.075

1.499 ± 0.022

9.918 ± 0.072

9.173 ± 0.076

9.539 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.03

6.83 ± 0.064

2.54 ± 0.028

2.474 ± 0.033

3.287 ± 0.04

6.196 ± 0.044

5.465 ± 0.066

6.576 ± 0.051

0.771 ± 0.017

4.836 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski