Bisgaardia hudsonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Bisgaardia

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1892 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2N0K5|A0A4R2N0K5_9PAST Protein transport protein HofB OS=Bisgaardia hudsonensis OX=109472 GN=EV697_103248 PE=3 SV=1
MM1 pKa = 7.43ALVGLFYY8 pKa = 11.01GSDD11 pKa = 3.18TGNTEE16 pKa = 3.78NVAKK20 pKa = 10.22MIQQQLGSEE29 pKa = 4.24LVDD32 pKa = 3.05IRR34 pKa = 11.84DD35 pKa = 3.46IAKK38 pKa = 8.47STKK41 pKa = 9.94EE42 pKa = 4.26DD43 pKa = 3.28IEE45 pKa = 5.11SYY47 pKa = 10.88DD48 pKa = 3.46FLMFGIPTWYY58 pKa = 10.25YY59 pKa = 11.58GEE61 pKa = 4.69AQCDD65 pKa = 3.26WDD67 pKa = 5.24DD68 pKa = 4.09FFPTLEE74 pKa = 4.91EE75 pKa = 4.17IDD77 pKa = 4.0FTDD80 pKa = 3.19KK81 pKa = 10.88LVAIFGCGDD90 pKa = 3.25QEE92 pKa = 5.04DD93 pKa = 4.3YY94 pKa = 11.79AEE96 pKa = 4.32YY97 pKa = 10.5FCDD100 pKa = 4.34AMGTVRR106 pKa = 11.84NIVEE110 pKa = 4.21PKK112 pKa = 8.64GAIIVGHH119 pKa = 7.12WSTEE123 pKa = 3.76GYY125 pKa = 10.3NFEE128 pKa = 4.13VSQALVDD135 pKa = 4.33DD136 pKa = 4.17DD137 pKa = 4.8TFVGLCIDD145 pKa = 4.19EE146 pKa = 4.82DD147 pKa = 3.95RR148 pKa = 11.84QPEE151 pKa = 4.37LTSQRR156 pKa = 11.84VEE158 pKa = 3.63QWVKK162 pKa = 9.6QVYY165 pKa = 10.73DD166 pKa = 4.03EE167 pKa = 4.42MCLAEE172 pKa = 4.85LVV174 pKa = 3.65

Molecular weight: 19.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2N242|A0A4R2N242_9PAST Alternative ribosome-rescue factor OS=Bisgaardia hudsonensis OX=109472 GN=EV697_10127 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.53RR12 pKa = 11.84SRR14 pKa = 11.84SHH16 pKa = 6.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1892

0

1892

619903

30

2769

327.6

36.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.202 ± 0.067

0.997 ± 0.019

5.177 ± 0.049

6.319 ± 0.056

4.467 ± 0.05

6.42 ± 0.061

1.937 ± 0.027

8.098 ± 0.064

6.889 ± 0.052

10.501 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.032

5.435 ± 0.056

3.509 ± 0.035

4.426 ± 0.048

4.098 ± 0.046

6.137 ± 0.05

5.288 ± 0.047

6.272 ± 0.048

1.127 ± 0.021

3.413 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski