Microthlaspi erraticum
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45482 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6D2JP36|A0A6D2JP36_9BRAS Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS29973 PE=4 SV=1
MM1 pKa = 7.46 EE2 pKa = 5.65 PVLQVLQQSHH12 pKa = 5.81 VLPPRR17 pKa = 11.84 DD18 pKa = 4.21 DD19 pKa = 3.87 VFFDD23 pKa = 4.12 DD24 pKa = 6.35 DD25 pKa = 4.17 EE26 pKa = 6.41 VDD28 pKa = 3.66 VLLKK32 pKa = 10.13 EE33 pKa = 3.8 IHH35 pKa = 6.34 GALIFDD41 pKa = 4.77 VYY43 pKa = 11.55 DD44 pKa = 3.58 DD45 pKa = 4.16 TNPISGACVYY55 pKa = 11.05 DD56 pKa = 3.45 EE57 pKa = 5.15 TIPILGVGDD66 pKa = 3.98 GTYY69 pKa = 10.53 LICDD73 pKa = 3.65 EE74 pKa = 5.05 GEE76 pKa = 3.97 NANPVDD82 pKa = 4.47 IISDD86 pKa = 4.1 EE87 pKa = 3.75 ISANVFIGVHH97 pKa = 4.48 EE98 pKa = 4.17 QEE100 pKa = 4.12 IEE102 pKa = 4.2 LIGVHH107 pKa = 6.04 EE108 pKa = 4.53 LLEE111 pKa = 4.37 SRR113 pKa = 11.84 NTNLFSRR120 pKa = 11.84 SDD122 pKa = 3.46 EE123 pKa = 4.11 DD124 pKa = 4.1 LSNNVCVTVVFDD136 pKa = 5.64 SYY138 pKa = 11.93 DD139 pKa = 3.8 DD140 pKa = 5.04 DD141 pKa = 5.66 PIFDD145 pKa = 4.53 FLLEE149 pKa = 4.19 KK150 pKa = 10.82 YY151 pKa = 9.67 VLRR154 pKa = 11.84 SLLTMRR160 pKa = 11.84 VQSSIPGITKK170 pKa = 9.8 IEE172 pKa = 3.98 AMTYY176 pKa = 6.48 TTQPTT181 pKa = 3.38
Molecular weight: 20.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.554
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.605
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A6D2JYA7|A0A6D2JYA7_9BRAS Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS32149 PE=4 SV=1
MM1 pKa = 7.42 SWRR4 pKa = 11.84 FAWRR8 pKa = 11.84 LWVVSAVLWCLGSSGVSRR26 pKa = 11.84 AWGAPVHH33 pKa = 6.62 GRR35 pKa = 11.84 FQRR38 pKa = 11.84 MGSSQGAPMRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 LGQGSGAWGSGRR62 pKa = 11.84 AQVRR66 pKa = 11.84 GRR68 pKa = 11.84 HH69 pKa = 5.09 RR70 pKa = 11.84 LGSGFTTIII79 pKa = 3.69
Molecular weight: 8.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.429
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.945
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45482
0
45482
16402413
10
5395
360.6
40.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.423 ± 0.009
1.832 ± 0.006
5.38 ± 0.008
6.821 ± 0.015
4.209 ± 0.007
6.331 ± 0.012
2.368 ± 0.005
5.044 ± 0.008
6.298 ± 0.012
9.42 ± 0.012
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.005
4.14 ± 0.008
5.11 ± 0.014
3.55 ± 0.009
5.893 ± 0.01
8.915 ± 0.013
5.138 ± 0.007
6.55 ± 0.009
1.367 ± 0.004
2.756 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here