Amino acid dipepetide frequency for Pseudomonas phage phiKMV

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.105AlaAla: 13.105 ± 1.367
1.002AlaCys: 1.002 ± 0.372
5.782AlaAsp: 5.782 ± 0.739
8.171AlaGlu: 8.171 ± 0.827
3.777AlaPhe: 3.777 ± 0.514
11.255AlaGly: 11.255 ± 1.246
1.85AlaHis: 1.85 ± 0.348
3.7AlaIle: 3.7 ± 0.638
6.244AlaLys: 6.244 ± 0.929
10.792AlaLeu: 10.792 ± 0.819
3.546AlaMet: 3.546 ± 0.522
3.315AlaAsn: 3.315 ± 0.669
4.934AlaPro: 4.934 ± 0.873
5.936AlaGln: 5.936 ± 0.739
5.319AlaArg: 5.319 ± 0.891
4.394AlaSer: 4.394 ± 0.564
6.398AlaThr: 6.398 ± 0.653
8.017AlaVal: 8.017 ± 0.991
1.156AlaTrp: 1.156 ± 0.204
3.238AlaTyr: 3.238 ± 0.581
0.0AlaXaa: 0.0 ± 0.0
Cys
1.002CysAla: 1.002 ± 0.305
0.077CysCys: 0.077 ± 0.08
0.463CysAsp: 0.463 ± 0.206
0.54CysGlu: 0.54 ± 0.176
0.231CysPhe: 0.231 ± 0.143
0.694CysGly: 0.694 ± 0.326
0.231CysHis: 0.231 ± 0.158
0.694CysIle: 0.694 ± 0.373
0.308CysLys: 0.308 ± 0.191
1.156CysLeu: 1.156 ± 0.309
0.308CysMet: 0.308 ± 0.171
0.308CysAsn: 0.308 ± 0.177
0.54CysPro: 0.54 ± 0.208
0.694CysGln: 0.694 ± 0.233
0.54CysArg: 0.54 ± 0.252
0.308CysSer: 0.308 ± 0.152
0.617CysThr: 0.617 ± 0.241
0.54CysVal: 0.54 ± 0.249
0.154CysTrp: 0.154 ± 0.173
0.617CysTyr: 0.617 ± 0.269
0.0CysXaa: 0.0 ± 0.0
Asp
6.475AspAla: 6.475 ± 0.663
0.463AspCys: 0.463 ± 0.185
2.929AspAsp: 2.929 ± 0.476
4.702AspGlu: 4.702 ± 0.555
1.773AspPhe: 1.773 ± 0.424
4.24AspGly: 4.24 ± 0.765
1.156AspHis: 1.156 ± 0.306
3.315AspIle: 3.315 ± 0.384
2.236AspLys: 2.236 ± 0.565
5.473AspLeu: 5.473 ± 0.579
1.619AspMet: 1.619 ± 0.307
1.85AspAsn: 1.85 ± 0.433
3.546AspPro: 3.546 ± 0.559
2.39AspGln: 2.39 ± 0.56
3.854AspArg: 3.854 ± 0.733
3.469AspSer: 3.469 ± 0.51
3.777AspThr: 3.777 ± 0.759
2.852AspVal: 2.852 ± 0.417
0.925AspTrp: 0.925 ± 0.234
1.696AspTyr: 1.696 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
9.636GluAla: 9.636 ± 1.091
0.154GluCys: 0.154 ± 0.114
2.621GluAsp: 2.621 ± 0.491
3.7GluGlu: 3.7 ± 0.605
2.158GluPhe: 2.158 ± 0.394
5.011GluGly: 5.011 ± 0.575
1.619GluHis: 1.619 ± 0.293
2.698GluIle: 2.698 ± 0.413
2.004GluLys: 2.004 ± 0.39
6.321GluLeu: 6.321 ± 0.69
2.158GluMet: 2.158 ± 0.453
1.773GluAsn: 1.773 ± 0.325
1.079GluPro: 1.079 ± 0.292
3.777GluGln: 3.777 ± 0.55
3.777GluArg: 3.777 ± 0.569
2.081GluSer: 2.081 ± 0.436
2.467GluThr: 2.467 ± 0.455
5.088GluVal: 5.088 ± 0.706
0.771GluTrp: 0.771 ± 0.219
2.081GluTyr: 2.081 ± 0.424
0.0GluXaa: 0.0 ± 0.0
Phe
2.39PheAla: 2.39 ± 0.453
0.385PheCys: 0.385 ± 0.18
2.852PheAsp: 2.852 ± 0.451
2.004PheGlu: 2.004 ± 0.426
1.079PhePhe: 1.079 ± 0.256
1.542PheGly: 1.542 ± 0.372
0.771PheHis: 0.771 ± 0.227
1.542PheIle: 1.542 ± 0.332
1.85PheLys: 1.85 ± 0.276
2.544PheLeu: 2.544 ± 0.384
0.925PheMet: 0.925 ± 0.262
1.465PheAsn: 1.465 ± 0.298
2.004PhePro: 2.004 ± 0.449
1.85PheGln: 1.85 ± 0.33
2.544PheArg: 2.544 ± 0.477
2.236PheSer: 2.236 ± 0.499
1.156PheThr: 1.156 ± 0.282
1.773PheVal: 1.773 ± 0.317
0.385PheTrp: 0.385 ± 0.169
0.617PheTyr: 0.617 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
6.938GlyAla: 6.938 ± 0.909
1.002GlyCys: 1.002 ± 0.245
3.854GlyAsp: 3.854 ± 0.491
4.009GlyGlu: 4.009 ± 0.487
2.852GlyPhe: 2.852 ± 0.613
5.396GlyGly: 5.396 ± 0.876
0.925GlyHis: 0.925 ± 0.263
4.317GlyIle: 4.317 ± 0.476
4.471GlyLys: 4.471 ± 0.732
7.401GlyLeu: 7.401 ± 0.934
2.621GlyMet: 2.621 ± 0.428
1.927GlyAsn: 1.927 ± 0.458
2.698GlyPro: 2.698 ± 0.514
3.623GlyGln: 3.623 ± 0.601
6.321GlyArg: 6.321 ± 0.698
4.317GlySer: 4.317 ± 0.448
5.396GlyThr: 5.396 ± 0.679
6.63GlyVal: 6.63 ± 0.681
1.85GlyTrp: 1.85 ± 0.514
2.544GlyTyr: 2.544 ± 0.486
0.0GlyXaa: 0.0 ± 0.0
His
1.079HisAla: 1.079 ± 0.265
0.231HisCys: 0.231 ± 0.131
1.311HisAsp: 1.311 ± 0.324
1.388HisGlu: 1.388 ± 0.384
0.617HisPhe: 0.617 ± 0.258
1.311HisGly: 1.311 ± 0.321
0.463HisHis: 0.463 ± 0.22
1.002HisIle: 1.002 ± 0.349
1.002HisLys: 1.002 ± 0.355
2.004HisLeu: 2.004 ± 0.407
0.463HisMet: 0.463 ± 0.226
1.002HisAsn: 1.002 ± 0.28
1.002HisPro: 1.002 ± 0.358
0.925HisGln: 0.925 ± 0.232
1.773HisArg: 1.773 ± 0.445
1.002HisSer: 1.002 ± 0.342
1.311HisThr: 1.311 ± 0.356
2.081HisVal: 2.081 ± 0.531
0.463HisTrp: 0.463 ± 0.157
0.617HisTyr: 0.617 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.859IleAla: 5.859 ± 0.775
0.385IleCys: 0.385 ± 0.197
3.315IleAsp: 3.315 ± 0.615
2.544IleGlu: 2.544 ± 0.304
1.156IlePhe: 1.156 ± 0.277
2.467IleGly: 2.467 ± 0.421
1.465IleHis: 1.465 ± 0.283
2.004IleIle: 2.004 ± 0.596
2.929IleLys: 2.929 ± 0.593
2.313IleLeu: 2.313 ± 0.566
1.311IleMet: 1.311 ± 0.301
2.004IleAsn: 2.004 ± 0.267
2.158IlePro: 2.158 ± 0.365
2.158IleGln: 2.158 ± 0.394
2.775IleArg: 2.775 ± 0.435
1.156IleSer: 1.156 ± 0.291
2.544IleThr: 2.544 ± 0.492
2.929IleVal: 2.929 ± 0.505
0.463IleTrp: 0.463 ± 0.207
0.771IleTyr: 0.771 ± 0.31
0.0IleXaa: 0.0 ± 0.0
Lys
6.167LysAla: 6.167 ± 0.627
0.308LysCys: 0.308 ± 0.155
3.469LysAsp: 3.469 ± 0.641
2.467LysGlu: 2.467 ± 0.511
1.542LysPhe: 1.542 ± 0.322
4.317LysGly: 4.317 ± 0.496
0.771LysHis: 0.771 ± 0.33
1.079LysIle: 1.079 ± 0.261
2.004LysLys: 2.004 ± 0.472
4.163LysLeu: 4.163 ± 0.834
1.927LysMet: 1.927 ± 0.31
1.233LysAsn: 1.233 ± 0.372
2.39LysPro: 2.39 ± 0.48
2.775LysGln: 2.775 ± 0.465
2.621LysArg: 2.621 ± 0.44
2.081LysSer: 2.081 ± 0.466
2.158LysThr: 2.158 ± 0.439
3.7LysVal: 3.7 ± 0.598
0.617LysTrp: 0.617 ± 0.2
1.002LysTyr: 1.002 ± 0.296
0.0LysXaa: 0.0 ± 0.0
Leu
10.253LeuAla: 10.253 ± 0.881
1.156LeuCys: 1.156 ± 0.369
6.784LeuAsp: 6.784 ± 0.631
5.473LeuGlu: 5.473 ± 0.701
2.004LeuPhe: 2.004 ± 0.435
7.632LeuGly: 7.632 ± 1.157
2.775LeuHis: 2.775 ± 0.566
3.546LeuIle: 3.546 ± 0.462
3.469LeuLys: 3.469 ± 0.343
7.169LeuLeu: 7.169 ± 0.714
2.313LeuMet: 2.313 ± 0.426
3.7LeuAsn: 3.7 ± 0.611
4.317LeuPro: 4.317 ± 0.646
3.238LeuGln: 3.238 ± 0.466
6.398LeuArg: 6.398 ± 0.783
5.242LeuSer: 5.242 ± 0.553
5.242LeuThr: 5.242 ± 0.715
5.396LeuVal: 5.396 ± 0.676
1.311LeuTrp: 1.311 ± 0.229
3.084LeuTyr: 3.084 ± 0.5
0.0LeuXaa: 0.0 ± 0.0
Met
4.086MetAla: 4.086 ± 0.597
0.0MetCys: 0.0 ± 0.0
2.004MetAsp: 2.004 ± 0.266
1.696MetGlu: 1.696 ± 0.45
0.463MetPhe: 0.463 ± 0.288
2.313MetGly: 2.313 ± 0.357
0.848MetHis: 0.848 ± 0.252
0.617MetIle: 0.617 ± 0.188
1.079MetLys: 1.079 ± 0.331
2.39MetLeu: 2.39 ± 0.513
0.925MetMet: 0.925 ± 0.331
1.388MetAsn: 1.388 ± 0.379
1.465MetPro: 1.465 ± 0.312
1.696MetGln: 1.696 ± 0.434
2.004MetArg: 2.004 ± 0.373
1.927MetSer: 1.927 ± 0.329
3.006MetThr: 3.006 ± 0.338
0.771MetVal: 0.771 ± 0.209
0.308MetTrp: 0.308 ± 0.149
0.925MetTyr: 0.925 ± 0.245
0.0MetXaa: 0.0 ± 0.0
Asn
3.777AsnAla: 3.777 ± 0.637
0.077AsnCys: 0.077 ± 0.081
1.619AsnAsp: 1.619 ± 0.358
2.081AsnGlu: 2.081 ± 0.278
0.848AsnPhe: 0.848 ± 0.298
2.544AsnGly: 2.544 ± 0.368
0.54AsnHis: 0.54 ± 0.221
1.002AsnIle: 1.002 ± 0.294
1.927AsnLys: 1.927 ± 0.392
3.623AsnLeu: 3.623 ± 0.559
0.848AsnMet: 0.848 ± 0.254
1.311AsnAsn: 1.311 ± 0.298
2.236AsnPro: 2.236 ± 0.495
1.542AsnGln: 1.542 ± 0.359
1.85AsnArg: 1.85 ± 0.391
1.465AsnSer: 1.465 ± 0.418
2.39AsnThr: 2.39 ± 0.554
3.623AsnVal: 3.623 ± 0.706
1.311AsnTrp: 1.311 ± 0.323
1.156AsnTyr: 1.156 ± 0.232
0.0AsnXaa: 0.0 ± 0.0
Pro
5.396ProAla: 5.396 ± 1.086
0.463ProCys: 0.463 ± 0.213
2.467ProAsp: 2.467 ± 0.536
4.086ProGlu: 4.086 ± 0.608
1.311ProPhe: 1.311 ± 0.315
3.932ProGly: 3.932 ± 0.527
0.694ProHis: 0.694 ± 0.23
1.465ProIle: 1.465 ± 0.334
2.544ProLys: 2.544 ± 0.333
4.009ProLeu: 4.009 ± 0.588
0.694ProMet: 0.694 ± 0.219
2.158ProAsn: 2.158 ± 0.473
1.773ProPro: 1.773 ± 0.569
1.773ProGln: 1.773 ± 0.901
2.621ProArg: 2.621 ± 0.478
2.158ProSer: 2.158 ± 0.409
2.929ProThr: 2.929 ± 0.448
3.315ProVal: 3.315 ± 0.831
1.002ProTrp: 1.002 ± 0.258
1.311ProTyr: 1.311 ± 0.266
0.0ProXaa: 0.0 ± 0.0
Gln
7.246GlnAla: 7.246 ± 0.99
0.617GlnCys: 0.617 ± 0.242
1.85GlnAsp: 1.85 ± 0.321
2.467GlnGlu: 2.467 ± 0.363
1.542GlnPhe: 1.542 ± 0.371
4.086GlnGly: 4.086 ± 0.471
0.925GlnHis: 0.925 ± 0.289
2.081GlnIle: 2.081 ± 0.288
1.156GlnLys: 1.156 ± 0.255
3.777GlnLeu: 3.777 ± 0.611
1.079GlnMet: 1.079 ± 0.271
1.465GlnAsn: 1.465 ± 0.265
1.85GlnPro: 1.85 ± 0.653
2.621GlnGln: 2.621 ± 0.548
3.546GlnArg: 3.546 ± 0.614
2.544GlnSer: 2.544 ± 0.462
2.004GlnThr: 2.004 ± 0.33
3.315GlnVal: 3.315 ± 0.561
0.694GlnTrp: 0.694 ± 0.268
1.927GlnTyr: 1.927 ± 0.443
0.0GlnXaa: 0.0 ± 0.0
Arg
6.861ArgAla: 6.861 ± 1.027
0.848ArgCys: 0.848 ± 0.334
4.394ArgAsp: 4.394 ± 0.484
3.854ArgGlu: 3.854 ± 0.646
3.084ArgPhe: 3.084 ± 0.554
5.011ArgGly: 5.011 ± 0.557
1.156ArgHis: 1.156 ± 0.347
3.238ArgIle: 3.238 ± 0.488
3.238ArgLys: 3.238 ± 0.631
5.55ArgLeu: 5.55 ± 0.658
2.467ArgMet: 2.467 ± 0.469
3.084ArgAsn: 3.084 ± 0.543
2.544ArgPro: 2.544 ± 0.542
2.467ArgGln: 2.467 ± 0.366
5.936ArgArg: 5.936 ± 0.681
3.932ArgSer: 3.932 ± 0.557
2.929ArgThr: 2.929 ± 0.508
4.24ArgVal: 4.24 ± 0.592
1.696ArgTrp: 1.696 ± 0.361
1.85ArgTyr: 1.85 ± 0.445
0.0ArgXaa: 0.0 ± 0.0
Ser
5.165SerAla: 5.165 ± 0.534
0.54SerCys: 0.54 ± 0.197
2.467SerAsp: 2.467 ± 0.396
2.929SerGlu: 2.929 ± 0.503
1.233SerPhe: 1.233 ± 0.289
4.24SerGly: 4.24 ± 0.561
0.771SerHis: 0.771 ± 0.275
2.621SerIle: 2.621 ± 0.608
2.544SerLys: 2.544 ± 0.41
5.55SerLeu: 5.55 ± 0.621
1.773SerMet: 1.773 ± 0.355
1.311SerAsn: 1.311 ± 0.28
2.004SerPro: 2.004 ± 0.316
2.236SerGln: 2.236 ± 0.426
4.394SerArg: 4.394 ± 0.549
1.619SerSer: 1.619 ± 0.494
2.158SerThr: 2.158 ± 0.644
3.315SerVal: 3.315 ± 0.461
0.694SerTrp: 0.694 ± 0.224
1.311SerTyr: 1.311 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
6.63ThrAla: 6.63 ± 0.969
0.848ThrCys: 0.848 ± 0.278
3.392ThrAsp: 3.392 ± 0.565
2.621ThrGlu: 2.621 ± 0.408
2.236ThrPhe: 2.236 ± 0.454
4.702ThrGly: 4.702 ± 0.676
1.465ThrHis: 1.465 ± 0.274
3.315ThrIle: 3.315 ± 0.445
3.006ThrLys: 3.006 ± 0.464
4.857ThrLeu: 4.857 ± 0.625
0.925ThrMet: 0.925 ± 0.267
1.927ThrAsn: 1.927 ± 0.409
3.7ThrPro: 3.7 ± 0.534
2.236ThrGln: 2.236 ± 0.37
2.929ThrArg: 2.929 ± 0.505
2.929ThrSer: 2.929 ± 0.455
2.929ThrThr: 2.929 ± 0.588
3.084ThrVal: 3.084 ± 0.545
0.694ThrTrp: 0.694 ± 0.211
1.85ThrTyr: 1.85 ± 0.378
0.0ThrXaa: 0.0 ± 0.0
Val
6.707ValAla: 6.707 ± 0.759
0.925ValCys: 0.925 ± 0.294
4.625ValAsp: 4.625 ± 0.714
3.854ValGlu: 3.854 ± 0.528
2.39ValPhe: 2.39 ± 0.453
5.088ValGly: 5.088 ± 0.663
1.388ValHis: 1.388 ± 0.445
2.775ValIle: 2.775 ± 0.752
3.238ValLys: 3.238 ± 0.521
7.015ValLeu: 7.015 ± 0.811
2.39ValMet: 2.39 ± 0.433
2.236ValAsn: 2.236 ± 0.367
3.469ValPro: 3.469 ± 1.129
2.775ValGln: 2.775 ± 0.549
5.628ValArg: 5.628 ± 0.668
2.621ValSer: 2.621 ± 0.497
3.854ValThr: 3.854 ± 0.629
4.625ValVal: 4.625 ± 0.68
0.385ValTrp: 0.385 ± 0.158
2.313ValTyr: 2.313 ± 0.453
0.0ValXaa: 0.0 ± 0.0
Trp
1.388TrpAla: 1.388 ± 0.378
0.308TrpCys: 0.308 ± 0.157
1.311TrpAsp: 1.311 ± 0.356
0.617TrpGlu: 0.617 ± 0.22
0.694TrpPhe: 0.694 ± 0.225
1.002TrpGly: 1.002 ± 0.346
0.231TrpHis: 0.231 ± 0.182
0.617TrpIle: 0.617 ± 0.278
0.617TrpLys: 0.617 ± 0.183
1.233TrpLeu: 1.233 ± 0.318
0.54TrpMet: 0.54 ± 0.194
1.002TrpAsn: 1.002 ± 0.266
0.694TrpPro: 0.694 ± 0.247
0.771TrpGln: 0.771 ± 0.317
1.311TrpArg: 1.311 ± 0.375
1.079TrpSer: 1.079 ± 0.29
0.848TrpThr: 0.848 ± 0.276
0.771TrpVal: 0.771 ± 0.307
0.231TrpTrp: 0.231 ± 0.206
0.617TrpTyr: 0.617 ± 0.219
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.773TyrAla: 1.773 ± 0.366
0.231TyrCys: 0.231 ± 0.186
1.233TyrAsp: 1.233 ± 0.379
1.773TyrGlu: 1.773 ± 0.314
0.925TyrPhe: 0.925 ± 0.244
2.313TyrGly: 2.313 ± 0.42
1.002TyrHis: 1.002 ± 0.246
1.079TyrIle: 1.079 ± 0.326
0.925TyrLys: 0.925 ± 0.326
3.238TyrLeu: 3.238 ± 0.45
1.002TyrMet: 1.002 ± 0.264
1.311TyrAsn: 1.311 ± 0.314
1.696TyrPro: 1.696 ± 0.385
1.311TyrGln: 1.311 ± 0.319
2.236TyrArg: 2.236 ± 0.381
2.313TyrSer: 2.313 ± 0.48
2.158TyrThr: 2.158 ± 0.45
2.236TyrVal: 2.236 ± 0.414
0.771TyrTrp: 0.771 ± 0.183
0.463TyrTyr: 0.463 ± 0.326
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (12973 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski