Myroides profundi
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3534 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5RHP8|A0A0B5RHP8_9FLAO Peptidase S9 OS=Myroides profundi OX=480520 GN=MPR_1081 PE=4 SV=1
MM1 pKa = 7.69 KK2 pKa = 10.74 LNTTNRR8 pKa = 11.84 ADD10 pKa = 3.82 LPNPKK15 pKa = 9.12 ILKK18 pKa = 8.47 EE19 pKa = 4.04 LCKK22 pKa = 10.74 SLATLDD28 pKa = 5.69 AILCQDD34 pKa = 3.0 WEE36 pKa = 4.24 YY37 pKa = 11.07 RR38 pKa = 11.84 YY39 pKa = 10.92 YY40 pKa = 10.99 LYY42 pKa = 11.38 NHH44 pKa = 7.2 LWSDD48 pKa = 3.84 DD49 pKa = 3.58 EE50 pKa = 5.68 EE51 pKa = 4.48 FFQMRR56 pKa = 11.84 DD57 pKa = 3.23 GEE59 pKa = 4.5 GNDD62 pKa = 3.25 MVILFRR68 pKa = 11.84 VDD70 pKa = 2.96 GTVINGFDD78 pKa = 3.25 HH79 pKa = 6.74 EE80 pKa = 5.18 LYY82 pKa = 10.74 DD83 pKa = 5.13 FEE85 pKa = 5.85 EE86 pKa = 4.2 NLPNKK91 pKa = 10.0 EE92 pKa = 4.46 DD93 pKa = 3.66 LTAGLPNQYY102 pKa = 9.92 IEE104 pKa = 5.23 FIFGEE109 pKa = 4.64 YY110 pKa = 9.83 ISDD113 pKa = 3.62 IGTTYY118 pKa = 10.64 CIWSDD123 pKa = 4.63 NNQQWAIGQVDD134 pKa = 3.69 NEE136 pKa = 4.46 EE137 pKa = 5.0 DD138 pKa = 3.82 GSDD141 pKa = 3.45 DD142 pKa = 3.91 QLYY145 pKa = 10.47 IFDD148 pKa = 5.15 NNPDD152 pKa = 3.65 TYY154 pKa = 11.22 INWATNYY161 pKa = 10.15 YY162 pKa = 9.99 FDD164 pKa = 4.68 EE165 pKa = 4.83 EE166 pKa = 4.67 EE167 pKa = 4.26 EE168 pKa = 4.95 SMTADD173 pKa = 3.0 KK174 pKa = 11.0 KK175 pKa = 11.16 DD176 pKa = 3.83 IIRR179 pKa = 11.84 QIYY182 pKa = 8.22 QGKK185 pKa = 7.27 TLTKK189 pKa = 10.79 EE190 pKa = 4.19 MILTLNPEE198 pKa = 4.18 LEE200 pKa = 4.1 DD201 pKa = 3.64 WEE203 pKa = 4.53 LLKK206 pKa = 10.94 EE207 pKa = 4.59 DD208 pKa = 4.59 IIEE211 pKa = 3.98 INYY214 pKa = 8.21 PHH216 pKa = 6.86 SLL218 pKa = 3.5
Molecular weight: 25.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.706
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.19
Thurlkill 3.745
EMBOSS 3.808
Sillero 4.024
Patrickios 1.252
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.911
Protein with the highest isoelectric point:
>tr|A0A0B5RWD5|A0A0B5RWD5_9FLAO Mechanosensitive ion channel protein MscS OS=Myroides profundi OX=480520 GN=MPR_1159 PE=4 SV=1
MM1 pKa = 8.03 IEE3 pKa = 4.28 DD4 pKa = 4.01 VIYY7 pKa = 9.97 NQWSIILGLLYY18 pKa = 10.3 TGWLTSRR25 pKa = 11.84 IVKK28 pKa = 8.88 RR29 pKa = 11.84 TTKK32 pKa = 9.96 GRR34 pKa = 11.84 KK35 pKa = 8.21 EE36 pKa = 3.56 RR37 pKa = 11.84 AGCVDD42 pKa = 4.06 VIRR45 pKa = 11.84 VFLIIGGLFGLTVFGSVAFGSMFIRR70 pKa = 11.84 TLSSLINGTVVFSIEE85 pKa = 3.87 LFAEE89 pKa = 4.69 LVCYY93 pKa = 9.18 GTMVAVLVFAFIGVMLYY110 pKa = 10.84 GVGRR114 pKa = 11.84 DD115 pKa = 3.14 MRR117 pKa = 11.84 RR118 pKa = 11.84 YY119 pKa = 9.54 NRR121 pKa = 11.84 LARR124 pKa = 11.84 LLGLGVLVPLIMLVFEE140 pKa = 4.65 GALVYY145 pKa = 10.96 ALVRR149 pKa = 11.84 NIHH152 pKa = 6.4 NIPLSVLLTVFILILLIGIILYY174 pKa = 9.86 VKK176 pKa = 10.17 IIRR179 pKa = 11.84 EE180 pKa = 4.27 EE181 pKa = 3.77 ITDD184 pKa = 4.79 LIEE187 pKa = 4.18 KK188 pKa = 9.12 EE189 pKa = 4.37 KK190 pKa = 10.8 NASRR194 pKa = 11.84 SKK196 pKa = 10.02 EE197 pKa = 3.93 NKK199 pKa = 8.75 TKK201 pKa = 11.03 GITQDD206 pKa = 3.11 KK207 pKa = 10.36 SVTRR211 pKa = 11.84 AQTANHH217 pKa = 5.98 SRR219 pKa = 11.84 QEE221 pKa = 4.5 SNRR224 pKa = 11.84 STQRR228 pKa = 11.84 TNKK231 pKa = 9.37 PNPHH235 pKa = 5.36 NDD237 pKa = 2.57 EE238 pKa = 3.72 WKK240 pKa = 9.99 RR241 pKa = 11.84 RR242 pKa = 11.84 SNRR245 pKa = 11.84 RR246 pKa = 11.84 KK247 pKa = 9.98 KK248 pKa = 10.38 RR249 pKa = 3.33
Molecular weight: 28.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.721
IPC_protein 10.409
Toseland 10.657
ProMoST 10.409
Dawson 10.76
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.052
Grimsley 10.804
Solomon 10.847
Lehninger 10.818
Nozaki 10.628
DTASelect 10.438
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.76
IPC_peptide 10.847
IPC2_peptide 9.297
IPC2.peptide.svr19 8.658
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3534
0
3534
1144917
37
3339
324.0
36.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.181 ± 0.049
0.727 ± 0.013
5.552 ± 0.032
6.822 ± 0.048
4.913 ± 0.033
6.246 ± 0.046
1.755 ± 0.021
7.968 ± 0.038
7.839 ± 0.04
9.449 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.338 ± 0.025
5.783 ± 0.043
3.125 ± 0.022
3.542 ± 0.025
3.39 ± 0.03
6.343 ± 0.035
5.798 ± 0.044
6.702 ± 0.038
0.975 ± 0.014
4.552 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here