Calocera cornea HHB12733
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13078 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A165H3I1|A0A165H3I1_9BASI Uncharacterized protein OS=Calocera cornea HHB12733 OX=1353952 GN=CALCODRAFT_228751 PE=4 SV=1
MM1 pKa = 7.4 FGSTLLTTVVAALFTSIVSASPVARR26 pKa = 11.84 QGVTVEE32 pKa = 4.89 LGWITNDD39 pKa = 3.35 KK40 pKa = 11.03 LGGAWTKK47 pKa = 7.44 MTQGNSSAGVPIGISPNTGGADD69 pKa = 3.05 IRR71 pKa = 11.84 QLFALYY77 pKa = 10.83 GDD79 pKa = 3.92 ASGDD83 pKa = 3.26 NTYY86 pKa = 10.41 TIIEE90 pKa = 5.1 LDD92 pKa = 3.12 TGNYY96 pKa = 9.66 VSTLQTTNSDD106 pKa = 3.01 TWSSGFEE113 pKa = 3.85 MLQTSTVAADD123 pKa = 3.26 NTVFKK128 pKa = 10.87 VSPGYY133 pKa = 10.38 DD134 pKa = 2.92 EE135 pKa = 4.95 GSWYY139 pKa = 9.73 IQPNTPSNTQLGWYY153 pKa = 9.97 SYY155 pKa = 10.73 DD156 pKa = 3.72 DD157 pKa = 3.81 PAAPFIPIILNEE169 pKa = 4.19 PLQEE173 pKa = 4.93 PDD175 pKa = 3.53 QSWTFHH181 pKa = 6.51 PLL183 pKa = 3.0
Molecular weight: 19.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.834
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.49
Solomon 3.77
Lehninger 3.719
Nozaki 3.91
DTASelect 4.151
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.91
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A165D7C3|A0A165D7C3_9BASI Uncharacterized protein OS=Calocera cornea HHB12733 OX=1353952 GN=CALCODRAFT_487314 PE=4 SV=1
QQ1 pKa = 7.0 LKK3 pKa = 10.4 HH4 pKa = 6.8 RR5 pKa = 11.84 FRR7 pKa = 11.84 QLKK10 pKa = 9.82 HH11 pKa = 6.48 RR12 pKa = 11.84 FRR14 pKa = 11.84 QLNHH18 pKa = 6.93 RR19 pKa = 11.84 FRR21 pKa = 11.84 QLKK24 pKa = 9.84 HH25 pKa = 6.45 RR26 pKa = 11.84 FRR28 pKa = 11.84 QLKK31 pKa = 9.82 HH32 pKa = 6.48 RR33 pKa = 11.84 FRR35 pKa = 11.84 QLNHH39 pKa = 6.61 RR40 pKa = 11.84 FSQRR44 pKa = 11.84 KK45 pKa = 8.03 HH46 pKa = 6.08 RR47 pKa = 11.84 FRR49 pKa = 11.84 QLNHH53 pKa = 6.72 RR54 pKa = 11.84 FSQLKK59 pKa = 10.01 HH60 pKa = 6.39 RR61 pKa = 11.84 FRR63 pKa = 11.84 QLNHH67 pKa = 6.72 RR68 pKa = 11.84 FSQLKK73 pKa = 9.33 HH74 pKa = 5.46
Molecular weight: 9.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.504
IPC2_protein 11.477
IPC_protein 13.071
Toseland 13.232
ProMoST 13.729
Dawson 13.232
Bjellqvist 13.232
Wikipedia 13.715
Rodwell 12.881
Grimsley 13.276
Solomon 13.729
Lehninger 13.627
Nozaki 13.232
DTASelect 13.232
Thurlkill 13.232
EMBOSS 13.729
Sillero 13.232
Patrickios 12.603
IPC_peptide 13.729
IPC2_peptide 12.72
IPC2.peptide.svr19 9.346
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13076
2
13078
5237295
49
5001
400.5
44.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.596 ± 0.022
1.279 ± 0.008
5.35 ± 0.017
6.061 ± 0.024
3.397 ± 0.013
7.107 ± 0.02
2.495 ± 0.01
4.291 ± 0.017
4.07 ± 0.02
9.473 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.009
2.944 ± 0.013
7.064 ± 0.028
3.824 ± 0.017
6.636 ± 0.025
7.808 ± 0.023
5.835 ± 0.014
6.34 ± 0.018
1.601 ± 0.009
2.609 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here