Psittacine adenovirus 3
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7JW93|A0A0A7JW93_9ADEN Uncharacterized protein OS=Psittacine adenovirus 3 OX=1580497 GN=PAV19gp24 PE=4 SV=1
MM1 pKa = 7.01 QPAAKK6 pKa = 9.87 RR7 pKa = 11.84 PRR9 pKa = 11.84 DD10 pKa = 3.47 EE11 pKa = 4.5 SSAVDD16 pKa = 3.45 LVYY19 pKa = 10.68 PFDD22 pKa = 4.0 EE23 pKa = 4.96 AVTAPLPPFIDD34 pKa = 3.08 VGGGLEE40 pKa = 4.2 TEE42 pKa = 4.31 GLKK45 pKa = 10.92 LSLNVSSPLTLRR57 pKa = 11.84 NNAVALKK64 pKa = 9.48 IGSGLSVDD72 pKa = 4.14 SSGALQAAAAASVSPPLSNSGGTLHH97 pKa = 7.01 LSVGSGLTVADD108 pKa = 3.92 GALASSLTASPPLSISGDD126 pKa = 3.66 TLALAVGNGLAVSGGVLASSLNPAPPLQLLGDD158 pKa = 4.68 SISLSLGSGLAVQSGALVSSPAVTAPVTITNGALGLSVGDD198 pKa = 3.53 GLTVRR203 pKa = 11.84 EE204 pKa = 4.81 GSLQAPTEE212 pKa = 4.15 MFSAVQGWDD221 pKa = 3.13 TLDD224 pKa = 3.47 NFSAVNIVSSAGVKK238 pKa = 9.95 FPASVFCEE246 pKa = 3.69 RR247 pKa = 11.84 LNIRR251 pKa = 11.84 GLVVCTMALKK261 pKa = 10.7 LDD263 pKa = 3.8 TSQWSSYY270 pKa = 10.52 GSTAPSSDD278 pKa = 3.07 FVFRR282 pKa = 11.84 LGEE285 pKa = 4.13 LFNSGVPSTYY295 pKa = 10.25 VPASAGSSYY304 pKa = 9.62 LTPHH308 pKa = 6.68 GGITTSTTSQSSGYY322 pKa = 9.77 VSPEE326 pKa = 3.43 GGTVVVFRR334 pKa = 11.84 PACSGAWANQFDD346 pKa = 4.21 VSQCTIVFPSEE357 pKa = 4.02 GGTVLHH363 pKa = 7.1 FSLQNKK369 pKa = 9.43 SGQTNYY375 pKa = 10.66 LFTPGITGTMLIYY388 pKa = 10.1 PISFSYY394 pKa = 10.55 LAANSLL400 pKa = 3.8
Molecular weight: 40.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.449
IPC2_protein 4.571
IPC_protein 4.495
Toseland 4.329
ProMoST 4.635
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.368
Rodwell 4.342
Grimsley 4.24
Solomon 4.469
Lehninger 4.418
Nozaki 4.584
DTASelect 4.787
Thurlkill 4.355
EMBOSS 4.38
Sillero 4.622
Patrickios 3.338
IPC_peptide 4.469
IPC2_peptide 4.609
IPC2.peptide.svr19 4.573
Protein with the highest isoelectric point:
>tr|A0A0A7JTS9|A0A0A7JTS9_9ADEN 100K OS=Psittacine adenovirus 3 OX=1580497 GN=PAV19gp15 PE=4 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 KK3 pKa = 8.11 RR4 pKa = 11.84 TGRR7 pKa = 11.84 SRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.22 RR12 pKa = 11.84 LSIVFGRR19 pKa = 11.84 PRR21 pKa = 11.84 LCRR24 pKa = 11.84 SNRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 LKK31 pKa = 10.98 GGFLPLLIPLISAAIGAAPAIASTVIAAKK60 pKa = 9.87 NARR63 pKa = 11.84 RR64 pKa = 11.84 HH65 pKa = 4.6
Molecular weight: 7.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.468
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.398
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.135
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.122
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
29
0
29
9690
54
1077
334.1
37.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.07 ± 0.484
2.632 ± 0.436
4.933 ± 0.265
5.459 ± 0.475
3.963 ± 0.208
6.347 ± 0.4
1.992 ± 0.219
4.076 ± 0.237
3.633 ± 0.405
9.556 ± 0.409
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.167 ± 0.158
4.241 ± 0.436
6.491 ± 0.307
3.849 ± 0.261
7.079 ± 0.576
7.833 ± 0.422
6.13 ± 0.341
6.584 ± 0.222
1.352 ± 0.126
3.612 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here