Circovirus-like genome DCCV-10
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A190WHE1|A0A190WHE1_9CIRC ATP-dependent helicase Rep OS=Circovirus-like genome DCCV-10 OX=1788438 PE=3 SV=1
MM1 pKa = 6.46 TTRR4 pKa = 11.84 IRR6 pKa = 11.84 AWCFTLNNPTDD17 pKa = 3.66 RR18 pKa = 11.84 DD19 pKa = 3.54 KK20 pKa = 11.43 RR21 pKa = 11.84 ALIALANTTRR31 pKa = 11.84 YY32 pKa = 10.22 VIAQLEE38 pKa = 4.3 HH39 pKa = 6.95 APTTGTPHH47 pKa = 5.39 YY48 pKa = 8.8 QGYY51 pKa = 7.88 MVFHH55 pKa = 7.6 DD56 pKa = 4.58 GKK58 pKa = 11.0 SFSALHH64 pKa = 6.09 KK65 pKa = 10.59 AMPRR69 pKa = 11.84 AHH71 pKa = 6.94 FEE73 pKa = 3.97 PARR76 pKa = 11.84 GTAQQNYY83 pKa = 9.89 DD84 pKa = 4.04 YY85 pKa = 9.02 CTKK88 pKa = 10.63 AADD91 pKa = 3.61 EE92 pKa = 4.55 GQPSNEE98 pKa = 3.81 LVLEE102 pKa = 4.64 HH103 pKa = 6.49 GTLPQQGEE111 pKa = 4.36 RR112 pKa = 11.84 VDD114 pKa = 3.37 ISQVRR119 pKa = 11.84 EE120 pKa = 3.89 LLRR123 pKa = 11.84 SGVTYY128 pKa = 10.79 RR129 pKa = 11.84 EE130 pKa = 4.09 LVDD133 pKa = 3.36 MDD135 pKa = 3.9 VNYY138 pKa = 10.35 QVLRR142 pKa = 11.84 IAQEE146 pKa = 3.74 WLRR149 pKa = 11.84 VHH151 pKa = 6.84 EE152 pKa = 4.48 PARR155 pKa = 11.84 DD156 pKa = 3.85 FKK158 pKa = 11.56 PEE160 pKa = 3.69 VRR162 pKa = 11.84 WFYY165 pKa = 11.44 GEE167 pKa = 4.04 PGAGKK172 pKa = 8.06 TRR174 pKa = 11.84 AALEE178 pKa = 3.93 WLEE181 pKa = 3.9 SHH183 pKa = 6.66 GEE185 pKa = 4.16 VFTVEE190 pKa = 4.33 TPAKK194 pKa = 9.98 YY195 pKa = 8.51 WDD197 pKa = 4.65 GYY199 pKa = 10.94 DD200 pKa = 3.29 GHH202 pKa = 7.14 NCVLFDD208 pKa = 5.79 DD209 pKa = 5.51 LRR211 pKa = 11.84 ADD213 pKa = 3.09 QCGFVRR219 pKa = 11.84 MLRR222 pKa = 11.84 LLDD225 pKa = 4.08 RR226 pKa = 11.84 YY227 pKa = 10.98 AMRR230 pKa = 11.84 VEE232 pKa = 4.22 VKK234 pKa = 10.52 GGSKK238 pKa = 8.18 QLRR241 pKa = 11.84 ARR243 pKa = 11.84 YY244 pKa = 8.81 IAITAPHH251 pKa = 6.83 RR252 pKa = 11.84 PEE254 pKa = 4.05 EE255 pKa = 4.16 IYY257 pKa = 9.43 RR258 pKa = 11.84 TSSEE262 pKa = 4.42 NIVQLTRR269 pKa = 11.84 RR270 pKa = 11.84 ISSVTHH276 pKa = 4.73 VTRR279 pKa = 11.84 DD280 pKa = 3.48 DD281 pKa = 4.17 PDD283 pKa = 3.33 NGRR286 pKa = 11.84 PTCRR290 pKa = 11.84 YY291 pKa = 9.17 GNCEE295 pKa = 4.14 PDD297 pKa = 3.11 EE298 pKa = 4.24 CLCRR302 pKa = 11.84 SNDD305 pKa = 3.88 GSDD308 pKa = 3.29 EE309 pKa = 4.79 LYY311 pKa = 10.95 DD312 pKa = 4.07 SEE314 pKa = 4.83
Molecular weight: 36.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.71
IPC2_protein 5.703
IPC_protein 5.753
Toseland 6.122
ProMoST 5.982
Dawson 6.008
Bjellqvist 6.02
Wikipedia 6.02
Rodwell 5.995
Grimsley 6.262
Solomon 6.008
Lehninger 6.008
Nozaki 6.275
DTASelect 6.465
Thurlkill 6.478
EMBOSS 6.44
Sillero 6.376
Patrickios 3.999
IPC_peptide 6.033
IPC2_peptide 6.389
IPC2.peptide.svr19 6.312
Protein with the highest isoelectric point:
>tr|A0A190WHC6|A0A190WHC6_9CIRC Uncharacterized protein OS=Circovirus-like genome DCCV-10 OX=1788438 PE=4 SV=1
MM1 pKa = 7.58 LQRR4 pKa = 11.84 QVHH7 pKa = 4.77 RR8 pKa = 11.84 TIKK11 pKa = 8.78 VTWSSTTVSRR21 pKa = 11.84 LVHH24 pKa = 5.23 CTRR27 pKa = 11.84 RR28 pKa = 11.84 CQEE31 pKa = 4.93 PISSLPGARR40 pKa = 11.84 RR41 pKa = 11.84 SKK43 pKa = 10.91 IMIIAQRR50 pKa = 11.84 LQTKK54 pKa = 9.9 ASPPTSSYY62 pKa = 11.17 SSTVPYY68 pKa = 9.74 PSRR71 pKa = 11.84 GRR73 pKa = 11.84 GSIYY77 pKa = 9.58 PRR79 pKa = 11.84 CVSYY83 pKa = 10.64 SAPGSHH89 pKa = 6.02 IVNWLTWMSITRR101 pKa = 11.84 SS102 pKa = 3.42
Molecular weight: 11.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.171
IPC2_protein 10.365
IPC_protein 11.491
Toseland 11.564
ProMoST 12.032
Dawson 11.594
Bjellqvist 11.564
Wikipedia 12.047
Rodwell 11.345
Grimsley 11.637
Solomon 12.047
Lehninger 11.945
Nozaki 11.564
DTASelect 11.564
Thurlkill 11.579
EMBOSS 12.062
Sillero 11.579
Patrickios 11.096
IPC_peptide 12.047
IPC2_peptide 11.038
IPC2.peptide.svr19 9.115
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
806
102
390
268.7
30.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.444 ± 0.533
1.613 ± 0.632
5.955 ± 0.845
4.218 ± 1.602
2.854 ± 0.465
5.583 ± 0.363
1.985 ± 0.89
4.094 ± 0.556
5.087 ± 1.382
7.32 ± 0.344
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.481 ± 0.27
3.846 ± 0.583
5.211 ± 0.405
4.467 ± 0.149
8.437 ± 1.136
8.065 ± 1.748
9.057 ± 1.024
7.072 ± 0.389
1.489 ± 0.27
3.722 ± 0.573
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here