Mumia flava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Mumia

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9BHD8|A0A2M9BHD8_9ACTN Flp pilus assembly protein TadB OS=Mumia flava OX=1348852 GN=CLV56_1596 PE=4 SV=1
MM1 pKa = 7.36GVADD5 pKa = 5.44DD6 pKa = 4.66EE7 pKa = 4.79GTHH10 pKa = 4.94VRR12 pKa = 11.84GRR14 pKa = 11.84RR15 pKa = 11.84LAVLAAATAILVAGCTSAQDD35 pKa = 3.49ARR37 pKa = 11.84GGDD40 pKa = 4.17DD41 pKa = 3.27PTAAATSSAADD52 pKa = 3.47VRR54 pKa = 11.84TPPDD58 pKa = 3.61PALAPYY64 pKa = 10.33YY65 pKa = 8.8EE66 pKa = 4.23QQVDD70 pKa = 3.03WRR72 pKa = 11.84SCDD75 pKa = 3.23GDD77 pKa = 3.36EE78 pKa = 4.53CADD81 pKa = 3.58VEE83 pKa = 5.28VPLDD87 pKa = 3.53YY88 pKa = 11.27ANPDD92 pKa = 3.43GEE94 pKa = 4.68RR95 pKa = 11.84IALRR99 pKa = 11.84LRR101 pKa = 11.84KK102 pKa = 9.17HH103 pKa = 4.78VAGRR107 pKa = 11.84PDD109 pKa = 3.72DD110 pKa = 3.96RR111 pKa = 11.84VGTLFVNPGGPGVSGVDD128 pKa = 3.32YY129 pKa = 10.58AAYY132 pKa = 9.14FPRR135 pKa = 11.84VAGEE139 pKa = 3.92AVTDD143 pKa = 3.83RR144 pKa = 11.84FDD146 pKa = 4.01VVGFDD151 pKa = 3.66PRR153 pKa = 11.84GVGASSALDD162 pKa = 3.72CGDD165 pKa = 3.47TAEE168 pKa = 3.93VDD170 pKa = 3.81AYY172 pKa = 11.48VEE174 pKa = 4.19TDD176 pKa = 3.35QTPDD180 pKa = 3.49DD181 pKa = 4.38ASEE184 pKa = 3.95EE185 pKa = 4.11QALVEE190 pKa = 4.2ASQTLGEE197 pKa = 4.08EE198 pKa = 4.65CEE200 pKa = 4.29RR201 pKa = 11.84GSGALASHH209 pKa = 6.8VSTVEE214 pKa = 3.47AARR217 pKa = 11.84DD218 pKa = 3.61LDD220 pKa = 3.74VLRR223 pKa = 11.84AVVDD227 pKa = 4.18DD228 pKa = 3.92QALHH232 pKa = 5.89YY233 pKa = 10.61LGASYY238 pKa = 8.2GTKK241 pKa = 10.42LGATYY246 pKa = 11.13AEE248 pKa = 4.48LFADD252 pKa = 3.69RR253 pKa = 11.84VGRR256 pKa = 11.84MVLDD260 pKa = 3.98GAMDD264 pKa = 3.65PTLDD268 pKa = 4.07SEE270 pKa = 4.91GVNLGQAEE278 pKa = 4.58GFQTALEE285 pKa = 4.63AYY287 pKa = 10.03VADD290 pKa = 4.02CVEE293 pKa = 4.31TAGCPLGEE301 pKa = 4.53DD302 pKa = 3.21TDD304 pKa = 4.36AGLEE308 pKa = 4.14EE309 pKa = 5.38IGDD312 pKa = 4.72LIASLDD318 pKa = 4.23DD319 pKa = 5.01DD320 pKa = 5.11PLPTSDD326 pKa = 4.23PDD328 pKa = 3.62RR329 pKa = 11.84PLTEE333 pKa = 4.11ALGFYY338 pKa = 10.44AVAYY342 pKa = 8.48PLYY345 pKa = 10.66NEE347 pKa = 4.81ASWPVLTEE355 pKa = 3.83ALADD359 pKa = 4.1GLDD362 pKa = 3.86GDD364 pKa = 4.78GDD366 pKa = 4.0TMLFVADD373 pKa = 3.96QYY375 pKa = 11.59LSRR378 pKa = 11.84GSDD381 pKa = 3.18GYY383 pKa = 9.79TDD385 pKa = 3.44NSTVAFPAISCLDD398 pKa = 3.48DD399 pKa = 3.81TGDD402 pKa = 3.37VSLDD406 pKa = 3.41EE407 pKa = 4.78ARR409 pKa = 11.84EE410 pKa = 4.0SLAEE414 pKa = 4.05FEE416 pKa = 5.05DD417 pKa = 4.04VSPVLGRR424 pKa = 11.84SFAWSAVGCSTWPMEE439 pKa = 4.34ATEE442 pKa = 4.46PAPEE446 pKa = 4.2IDD448 pKa = 3.91AAGAPPILVVGTTRR462 pKa = 11.84DD463 pKa = 3.33PATPYY468 pKa = 10.89AWAEE472 pKa = 3.9ALADD476 pKa = 3.8QLDD479 pKa = 3.97SGVLVTRR486 pKa = 11.84DD487 pKa = 3.39GDD489 pKa = 3.76GHH491 pKa = 5.96TGYY494 pKa = 10.91AAGNGCVDD502 pKa = 3.65AAVDD506 pKa = 4.02EE507 pKa = 4.43YY508 pKa = 11.47LVDD511 pKa = 3.66GTVPEE516 pKa = 5.66DD517 pKa = 3.72GLEE520 pKa = 4.12CC521 pKa = 5.3

Molecular weight: 54.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9BKK2|A0A2M9BKK2_9ACTN Succinate dehydrogenase / fumarate reductase flavoprotein subunit OS=Mumia flava OX=1348852 GN=CLV56_2715 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.68KK16 pKa = 9.77HH17 pKa = 5.59RR18 pKa = 11.84KK19 pKa = 7.37LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4037

0

4037

1354464

29

3061

335.5

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.653 ± 0.059

0.665 ± 0.01

6.954 ± 0.038

5.65 ± 0.033

2.662 ± 0.023

9.224 ± 0.032

2.036 ± 0.018

3.392 ± 0.025

1.612 ± 0.03

9.998 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.756 ± 0.013

1.567 ± 0.021

5.634 ± 0.031

2.492 ± 0.021

7.971 ± 0.047

5.294 ± 0.026

6.214 ± 0.042

9.685 ± 0.039

1.514 ± 0.017

2.025 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski