Pseudoalteromonas translucida (strain TAC 125)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas; Pseudoalteromonas translucida

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3IGW0|Q3IGW0_PSET1 Succinate dehydrogenase hydrophobic membrane anchor subunit OS=Pseudoalteromonas translucida (strain TAC 125) OX=326442 GN=sdhD PE=4 SV=1
MM1 pKa = 7.72IKK3 pKa = 9.13TLSLSTLALVTLGFNTAAKK22 pKa = 10.47ADD24 pKa = 3.6IADD27 pKa = 3.8FDD29 pKa = 4.09SPRR32 pKa = 11.84IYY34 pKa = 10.51TGVGYY39 pKa = 9.78GQYY42 pKa = 10.61SFEE45 pKa = 5.25FEE47 pKa = 5.7DD48 pKa = 3.93SDD50 pKa = 5.32KK51 pKa = 11.33DD52 pKa = 3.7TDD54 pKa = 4.2FDD56 pKa = 6.64DD57 pKa = 4.83DD58 pKa = 3.87AQMLKK63 pKa = 10.5GYY65 pKa = 10.36VGAQFNEE72 pKa = 4.17YY73 pKa = 10.59LSLEE77 pKa = 4.04LAYY80 pKa = 10.9QNFDD84 pKa = 3.27EE85 pKa = 5.31VSDD88 pKa = 3.79VDD90 pKa = 4.55SKK92 pKa = 12.13AEE94 pKa = 3.71IDD96 pKa = 4.21GVSLAARR103 pKa = 11.84LGYY106 pKa = 9.61PITEE110 pKa = 4.29SFSVYY115 pKa = 10.97AKK117 pKa = 10.67GGWLEE122 pKa = 3.56WDD124 pKa = 3.4AEE126 pKa = 4.42YY127 pKa = 9.27TQDD130 pKa = 4.26LPVGSVSASTDD141 pKa = 3.1GGDD144 pKa = 3.23AFYY147 pKa = 10.87GAGIEE152 pKa = 4.3YY153 pKa = 10.77ALTTNVQFRR162 pKa = 11.84LEE164 pKa = 4.0YY165 pKa = 10.44EE166 pKa = 4.13RR167 pKa = 11.84YY168 pKa = 10.2KK169 pKa = 10.9LDD171 pKa = 5.12DD172 pKa = 5.96DD173 pKa = 4.91IDD175 pKa = 5.6PDD177 pKa = 3.53MDD179 pKa = 3.73VASVSVQYY187 pKa = 10.04MFF189 pKa = 5.16

Molecular weight: 20.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3IKF3|Q3IKF3_PSET1 Putative orphan protein OS=Pseudoalteromonas translucida (strain TAC 125) OX=326442 GN=PSHAa1112 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVNGRR28 pKa = 11.84KK29 pKa = 9.18VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3484

0

3484

1116400

20

2410

320.4

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.953 ± 0.042

0.996 ± 0.013

5.385 ± 0.036

5.694 ± 0.043

4.302 ± 0.032

6.424 ± 0.042

2.2 ± 0.02

6.756 ± 0.042

5.847 ± 0.038

10.504 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.018

4.997 ± 0.026

3.664 ± 0.021

4.825 ± 0.04

3.978 ± 0.028

6.629 ± 0.031

5.555 ± 0.032

6.64 ± 0.038

1.101 ± 0.017

3.176 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski