Cupriavidus basilensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae;

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C4YNQ7|A0A0C4YNQ7_9BURK Hpa2 protein OS=Cupriavidus basilensis OX=68895 GN=RR42_s0630 PE=4 SV=1
MM1 pKa = 6.4QQEE4 pKa = 4.13AVVYY8 pKa = 7.39KK9 pKa = 9.74TWVCLICGWVYY20 pKa = 11.08DD21 pKa = 4.83EE22 pKa = 4.29EE23 pKa = 4.75QGWPDD28 pKa = 4.15DD29 pKa = 4.55GIAPGTRR36 pKa = 11.84WDD38 pKa = 5.76DD39 pKa = 3.35IPEE42 pKa = 4.0DD43 pKa = 3.91WRR45 pKa = 11.84CPEE48 pKa = 4.1CDD50 pKa = 3.13VGKK53 pKa = 11.2GEE55 pKa = 4.29FAMIEE60 pKa = 3.88II61 pKa = 4.85

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C4YFU5|A0A0C4YFU5_9BURK Response regulatory domain-containing protein OS=Cupriavidus basilensis OX=68895 GN=RR42_s0007 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.96TRR25 pKa = 11.84GGRR28 pKa = 11.84SVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7643

0

7643

2361659

37

4493

309.0

33.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.386 ± 0.044

0.99 ± 0.011

5.123 ± 0.022

5.04 ± 0.027

3.49 ± 0.018

8.609 ± 0.032

2.24 ± 0.015

4.441 ± 0.021

3.005 ± 0.024

10.477 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.013

2.623 ± 0.02

5.318 ± 0.021

3.856 ± 0.018

7.022 ± 0.029

5.346 ± 0.02

5.171 ± 0.024

7.574 ± 0.023

1.378 ± 0.012

2.359 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski