Trichinella papuae
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15285 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V1N5L1|A0A0V1N5L1_9BILA Cytoskeleton-associated protein 5 OS=Trichinella papuae OX=268474 GN=Ckap5 PE=4 SV=1
MM1 pKa = 7.5 AGVPPFDD8 pKa = 3.28 VPSFNFYY15 pKa = 11.35 VEE17 pKa = 4.86 FIKK20 pKa = 10.46 ILPKK24 pKa = 10.66 NFVHH28 pKa = 6.13 RR29 pKa = 11.84 TYY31 pKa = 10.94 NIQTEE36 pKa = 4.73 TEE38 pKa = 4.12 EE39 pKa = 4.48 STHH42 pKa = 6.63 SSQLYY47 pKa = 8.13 EE48 pKa = 3.96 VEE50 pKa = 4.46 YY51 pKa = 10.35 PMGMYY56 pKa = 10.07 NPKK59 pKa = 10.1 QPFEE63 pKa = 4.11 VEE65 pKa = 3.57 RR66 pKa = 11.84 FEE68 pKa = 4.44 IRR70 pKa = 11.84 DD71 pKa = 3.56 GAGEE75 pKa = 3.97 DD76 pKa = 3.68 AEE78 pKa = 4.96 EE79 pKa = 3.94 IAAVQQHH86 pKa = 5.92 EE87 pKa = 4.56 EE88 pKa = 4.33 DD89 pKa = 3.88 FGDD92 pKa = 3.38 RR93 pKa = 11.84 TPAEE97 pKa = 4.61 EE98 pKa = 4.28 YY99 pKa = 8.61 TSSVASYY106 pKa = 11.59 SNASPNDD113 pKa = 3.96 DD114 pKa = 5.69 DD115 pKa = 7.26 DD116 pKa = 7.58 DD117 pKa = 7.16 DD118 pKa = 7.3 DD119 pKa = 7.05 DD120 pKa = 4.79 DD121 pKa = 5.59 ASSIEE126 pKa = 3.58 ADD128 pKa = 3.85 YY129 pKa = 11.41 YY130 pKa = 11.39 FEE132 pKa = 4.31
Molecular weight: 15.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.579
ProMoST 3.897
Dawson 3.757
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.617
EMBOSS 3.668
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A0V1LZC4|A0A0V1LZC4_9BILA Uncharacterized protein OS=Trichinella papuae OX=268474 GN=T10_4483 PE=4 SV=1
MM1 pKa = 7.42 QNFHH5 pKa = 7.03 SLGANIATPQAMPQAIFQNFRR26 pKa = 11.84 IFRR29 pKa = 11.84 NFSPRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 PRR38 pKa = 11.84 LRR40 pKa = 11.84 LRR42 pKa = 11.84 AA43 pKa = 3.74
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11419
3866
15285
7709026
21
16819
504.4
57.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.447 ± 0.015
2.641 ± 0.02
5.225 ± 0.014
6.389 ± 0.025
4.571 ± 0.016
4.892 ± 0.023
2.465 ± 0.008
5.594 ± 0.016
6.091 ± 0.021
9.735 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.009
5.099 ± 0.014
4.284 ± 0.019
4.361 ± 0.013
5.521 ± 0.018
8.339 ± 0.022
5.281 ± 0.014
6.354 ± 0.014
1.183 ± 0.006
3.1 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here