Amino acid dipepetide frequency for Polaribacter sp. Hel1_33_78

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.14AlaAla: 4.14 ± 0.1	0.503AlaCys: 0.503 ± 0.029	3.218AlaAsp: 3.218 ± 0.091	3.62AlaGlu: 3.62 ± 0.068	3.179AlaPhe: 3.179 ± 0.064	3.998AlaGly: 3.998 ± 0.079	1.032AlaHis: 1.032 ± 0.036	5.68AlaIle: 5.68 ± 0.082	4.95AlaLys: 4.95 ± 0.094	5.51AlaLeu: 5.51 ± 0.092	1.406AlaMet: 1.406 ± 0.044	3.494AlaAsn: 3.494 ± 0.092	1.656AlaPro: 1.656 ± 0.048	2.087AlaGln: 2.087 ± 0.053	1.843AlaArg: 1.843 ± 0.048	4.229AlaSer: 4.229 ± 0.077	3.606AlaThr: 3.606 ± 0.091	3.891AlaVal: 3.891 ± 0.068	0.569AlaTrp: 0.569 ± 0.029	2.102AlaTyr: 2.102 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.458CysAla: 0.458 ± 0.039	0.099CysCys: 0.099 ± 0.011	0.408CysAsp: 0.408 ± 0.036	0.412CysGlu: 0.412 ± 0.021	0.396CysPhe: 0.396 ± 0.022	0.582CysGly: 0.582 ± 0.03	0.157CysHis: 0.157 ± 0.014	0.58CysIle: 0.58 ± 0.029	0.531CysLys: 0.531 ± 0.027	0.586CysLeu: 0.586 ± 0.028	0.13CysMet: 0.13 ± 0.01	0.427CysAsn: 0.427 ± 0.023	0.275CysPro: 0.275 ± 0.019	0.203CysGln: 0.203 ± 0.014	0.174CysArg: 0.174 ± 0.013	0.531CysSer: 0.531 ± 0.025	0.406CysThr: 0.406 ± 0.024	0.392CysVal: 0.392 ± 0.022	0.066CysTrp: 0.066 ± 0.007	0.236CysTyr: 0.236 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.559AspAla: 3.559 ± 0.074	0.393AspCys: 0.393 ± 0.03	2.737AspAsp: 2.737 ± 0.083	3.539AspGlu: 3.539 ± 0.063	3.733AspPhe: 3.733 ± 0.07	3.518AspGly: 3.518 ± 0.116	0.764AspHis: 0.764 ± 0.029	4.576AspIle: 4.576 ± 0.077	4.316AspLys: 4.316 ± 0.085	5.179AspLeu: 5.179 ± 0.074	0.932AspMet: 0.932 ± 0.035	3.168AspAsn: 3.168 ± 0.075	1.396AspPro: 1.396 ± 0.047	1.243AspGln: 1.243 ± 0.039	1.637AspArg: 1.637 ± 0.038	3.065AspSer: 3.065 ± 0.064	2.75AspThr: 2.75 ± 0.071	3.717AspVal: 3.717 ± 0.114	0.722AspTrp: 0.722 ± 0.028	2.459AspTyr: 2.459 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	3.883GluAla: 3.883 ± 0.079	0.276GluCys: 0.276 ± 0.017	3.376GluAsp: 3.376 ± 0.066	4.628GluGlu: 4.628 ± 0.094	3.058GluPhe: 3.058 ± 0.064	3.479GluGly: 3.479 ± 0.064	1.011GluHis: 1.011 ± 0.033	6.173GluIle: 6.173 ± 0.086	6.334GluLys: 6.334 ± 0.105	5.875GluLeu: 5.875 ± 0.092	1.564GluMet: 1.564 ± 0.042	5.321GluAsn: 5.321 ± 0.086	1.317GluPro: 1.317 ± 0.043	1.953GluGln: 1.953 ± 0.046	2.202GluArg: 2.202 ± 0.058	3.254GluSer: 3.254 ± 0.055	3.73GluThr: 3.73 ± 0.061	4.124GluVal: 4.124 ± 0.066	0.574GluTrp: 0.574 ± 0.026	2.326GluTyr: 2.326 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	2.876PheAla: 2.876 ± 0.057	0.43PheCys: 0.43 ± 0.021	3.386PheAsp: 3.386 ± 0.064	3.257PheGlu: 3.257 ± 0.065	2.992PhePhe: 2.992 ± 0.08	3.921PheGly: 3.921 ± 0.065	0.854PheHis: 0.854 ± 0.028	4.52PheIle: 4.52 ± 0.081	4.414PheLys: 4.414 ± 0.082	5.216PheLeu: 5.216 ± 0.115	1.127PheMet: 1.127 ± 0.035	3.561PheAsn: 3.561 ± 0.072	1.751PhePro: 1.751 ± 0.046	1.603PheGln: 1.603 ± 0.045	1.555PheArg: 1.555 ± 0.045	4.54PheSer: 4.54 ± 0.08	3.286PheThr: 3.286 ± 0.064	3.087PheVal: 3.087 ± 0.07	0.585PheTrp: 0.585 ± 0.024	2.314PheTyr: 2.314 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.151GlyAla: 4.151 ± 0.089	0.529GlyCys: 0.529 ± 0.027	3.22GlyAsp: 3.22 ± 0.08	3.367GlyGlu: 3.367 ± 0.075	3.915GlyPhe: 3.915 ± 0.084	4.737GlyGly: 4.737 ± 0.161	1.08GlyHis: 1.08 ± 0.033	5.576GlyIle: 5.576 ± 0.074	5.232GlyLys: 5.232 ± 0.09	5.543GlyLeu: 5.543 ± 0.08	1.51GlyMet: 1.51 ± 0.047	3.938GlyAsn: 3.938 ± 0.085	1.22GlyPro: 1.22 ± 0.033	1.662GlyGln: 1.662 ± 0.051	1.993GlyArg: 1.993 ± 0.053	4.045GlySer: 4.045 ± 0.125	3.914GlyThr: 3.914 ± 0.121	4.271GlyVal: 4.271 ± 0.089	0.729GlyTrp: 0.729 ± 0.032	2.56GlyTyr: 2.56 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	0.901HisAla: 0.901 ± 0.032	0.147HisCys: 0.147 ± 0.011	0.725HisAsp: 0.725 ± 0.025	0.899HisGlu: 0.899 ± 0.036	1.151HisPhe: 1.151 ± 0.035	0.996HisGly: 0.996 ± 0.03	0.482HisHis: 0.482 ± 0.028	1.46HisIle: 1.46 ± 0.044	1.407HisLys: 1.407 ± 0.039	1.637HisLeu: 1.637 ± 0.048	0.281HisMet: 0.281 ± 0.018	0.945HisAsn: 0.945 ± 0.031	0.846HisPro: 0.846 ± 0.03	0.766HisGln: 0.766 ± 0.03	0.631HisArg: 0.631 ± 0.025	0.976HisSer: 0.976 ± 0.033	0.931HisThr: 0.931 ± 0.03	0.8HisVal: 0.8 ± 0.033	0.201HisTrp: 0.201 ± 0.017	0.742HisTyr: 0.742 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.823IleAla: 5.823 ± 0.08	0.64IleCys: 0.64 ± 0.029	5.191IleAsp: 5.191 ± 0.074	5.777IleGlu: 5.777 ± 0.095	4.176IlePhe: 4.176 ± 0.084	5.336IleGly: 5.336 ± 0.091	1.493IleHis: 1.493 ± 0.04	7.319IleIle: 7.319 ± 0.111	7.055IleLys: 7.055 ± 0.098	7.752IleLeu: 7.752 ± 0.113	1.449IleMet: 1.449 ± 0.046	5.593IleAsn: 5.593 ± 0.1	3.155IlePro: 3.155 ± 0.063	2.676IleGln: 2.676 ± 0.054	2.617IleArg: 2.617 ± 0.05	6.444IleSer: 6.444 ± 0.078	5.297IleThr: 5.297 ± 0.101	4.878IleVal: 4.878 ± 0.072	0.738IleTrp: 0.738 ± 0.029	3.006IleTyr: 3.006 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	4.765LysAla: 4.765 ± 0.077	0.348LysCys: 0.348 ± 0.021	4.573LysAsp: 4.573 ± 0.068	7.378LysGlu: 7.378 ± 0.129	3.411LysPhe: 3.411 ± 0.066	4.959LysGly: 4.959 ± 0.08	1.449LysHis: 1.449 ± 0.043	7.758LysIle: 7.758 ± 0.108	8.695LysLys: 8.695 ± 0.147	7.024LysLeu: 7.024 ± 0.097	2.215LysMet: 2.215 ± 0.056	6.688LysAsn: 6.688 ± 0.095	2.382LysPro: 2.382 ± 0.055	2.843LysGln: 2.843 ± 0.057	3.044LysArg: 3.044 ± 0.068	4.978LysSer: 4.978 ± 0.088	5.159LysThr: 5.159 ± 0.078	5.183LysVal: 5.183 ± 0.088	0.808LysTrp: 0.808 ± 0.037	3.299LysTyr: 3.299 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	5.402LeuAla: 5.402 ± 0.1	0.599LeuCys: 0.599 ± 0.027	4.911LeuAsp: 4.911 ± 0.081	6.256LeuGlu: 6.256 ± 0.093	5.199LeuPhe: 5.199 ± 0.094	5.745LeuGly: 5.745 ± 0.094	1.46LeuHis: 1.46 ± 0.042	7.407LeuIle: 7.407 ± 0.128	8.371LeuLys: 8.371 ± 0.123	8.291LeuLeu: 8.291 ± 0.145	1.872LeuMet: 1.872 ± 0.044	5.762LeuAsn: 5.762 ± 0.085	3.234LeuPro: 3.234 ± 0.061	3.057LeuGln: 3.057 ± 0.069	2.936LeuArg: 2.936 ± 0.066	6.596LeuSer: 6.596 ± 0.09	4.929LeuThr: 4.929 ± 0.082	5.173LeuVal: 5.173 ± 0.086	0.729LeuTrp: 0.729 ± 0.029	2.934LeuTyr: 2.934 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	1.416MetAla: 1.416 ± 0.043	0.135MetCys: 0.135 ± 0.012	1.009MetAsp: 1.009 ± 0.033	1.186MetGlu: 1.186 ± 0.039	0.917MetPhe: 0.917 ± 0.033	1.277MetGly: 1.277 ± 0.045	0.408MetHis: 0.408 ± 0.019	1.71MetIle: 1.71 ± 0.044	2.238MetLys: 2.238 ± 0.05	1.839MetLeu: 1.839 ± 0.05	0.58MetMet: 0.58 ± 0.028	1.392MetAsn: 1.392 ± 0.042	0.755MetPro: 0.755 ± 0.031	0.792MetGln: 0.792 ± 0.03	0.78MetArg: 0.78 ± 0.03	1.396MetSer: 1.396 ± 0.039	1.065MetThr: 1.065 ± 0.033	1.154MetVal: 1.154 ± 0.036	0.157MetTrp: 0.157 ± 0.014	0.69MetTyr: 0.69 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.779AsnAla: 3.779 ± 0.073	0.505AsnCys: 0.505 ± 0.027	3.326AsnAsp: 3.326 ± 0.08	3.703AsnGlu: 3.703 ± 0.064	3.575AsnPhe: 3.575 ± 0.069	4.112AsnGly: 4.112 ± 0.121	1.163AsnHis: 1.163 ± 0.039	5.615AsnIle: 5.615 ± 0.085	5.421AsnLys: 5.421 ± 0.089	5.972AsnLeu: 5.972 ± 0.096	1.268AsnMet: 1.268 ± 0.039	4.523AsnAsn: 4.523 ± 0.117	2.82AsnPro: 2.82 ± 0.052	2.421AsnGln: 2.421 ± 0.054	2.122AsnArg: 2.122 ± 0.052	4.509AsnSer: 4.509 ± 0.087	3.946AsnThr: 3.946 ± 0.115	3.522AsnVal: 3.522 ± 0.063	0.847AsnTrp: 0.847 ± 0.03	3.037AsnTyr: 3.037 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	1.745ProAla: 1.745 ± 0.069	0.195ProCys: 0.195 ± 0.016	1.693ProAsp: 1.693 ± 0.049	2.355ProGlu: 2.355 ± 0.048	1.895ProPhe: 1.895 ± 0.046	1.668ProGly: 1.668 ± 0.05	0.565ProHis: 0.565 ± 0.025	2.723ProIle: 2.723 ± 0.061	2.752ProLys: 2.752 ± 0.056	2.689ProLeu: 2.689 ± 0.055	0.657ProMet: 0.657 ± 0.027	2.27ProAsn: 2.27 ± 0.055	0.692ProPro: 0.692 ± 0.027	0.895ProGln: 0.895 ± 0.028	0.851ProArg: 0.851 ± 0.028	2.041ProSer: 2.041 ± 0.045	1.955ProThr: 1.955 ± 0.052	1.934ProVal: 1.934 ± 0.058	0.353ProTrp: 0.353 ± 0.022	1.185ProTyr: 1.185 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	1.622GlnAla: 1.622 ± 0.041	0.139GlnCys: 0.139 ± 0.012	1.493GlnAsp: 1.493 ± 0.039	2.316GlnGlu: 2.316 ± 0.054	1.773GlnPhe: 1.773 ± 0.044	1.65GlnGly: 1.65 ± 0.047	0.562GlnHis: 0.562 ± 0.028	2.749GlnIle: 2.749 ± 0.061	3.164GlnLys: 3.164 ± 0.053	3.109GlnLeu: 3.109 ± 0.058	0.724GlnMet: 0.724 ± 0.025	2.223GlnAsn: 2.223 ± 0.048	0.897GlnPro: 0.897 ± 0.035	1.376GlnGln: 1.376 ± 0.044	1.102GlnArg: 1.102 ± 0.039	1.758GlnSer: 1.758 ± 0.039	1.835GlnThr: 1.835 ± 0.061	1.752GlnVal: 1.752 ± 0.04	0.295GlnTrp: 0.295 ± 0.017	1.208GlnTyr: 1.208 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	1.96ArgAla: 1.96 ± 0.051	0.176ArgCys: 0.176 ± 0.013	1.578ArgAsp: 1.578 ± 0.046	2.054ArgGlu: 2.054 ± 0.048	1.804ArgPhe: 1.804 ± 0.054	1.885ArgGly: 1.885 ± 0.045	0.549ArgHis: 0.549 ± 0.026	2.85ArgIle: 2.85 ± 0.056	2.968ArgLys: 2.968 ± 0.057	2.878ArgLeu: 2.878 ± 0.058	0.804ArgMet: 0.804 ± 0.031	2.13ArgAsn: 2.13 ± 0.05	0.935ArgPro: 0.935 ± 0.031	0.959ArgGln: 0.959 ± 0.03	1.187ArgArg: 1.187 ± 0.039	1.725ArgSer: 1.725 ± 0.044	1.74ArgThr: 1.74 ± 0.046	1.938ArgVal: 1.938 ± 0.043	0.307ArgTrp: 0.307 ± 0.018	1.34ArgTyr: 1.34 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.753SerAla: 3.753 ± 0.068	0.65SerCys: 0.65 ± 0.028	3.512SerAsp: 3.512 ± 0.072	4.262SerGlu: 4.262 ± 0.071	4.241SerPhe: 4.241 ± 0.079	4.702SerGly: 4.702 ± 0.128	1.011SerHis: 1.011 ± 0.035	5.862SerIle: 5.862 ± 0.101	5.772SerLys: 5.772 ± 0.08	6.182SerLeu: 6.182 ± 0.106	1.278SerMet: 1.278 ± 0.033	4.196SerAsn: 4.196 ± 0.083	1.907SerPro: 1.907 ± 0.047	1.948SerGln: 1.948 ± 0.046	1.854SerArg: 1.854 ± 0.05	4.593SerSer: 4.593 ± 0.087	3.502SerThr: 3.502 ± 0.077	3.82SerVal: 3.82 ± 0.072	0.795SerTrp: 0.795 ± 0.031	2.765SerTyr: 2.765 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	3.779ThrAla: 3.779 ± 0.108	0.345ThrCys: 0.345 ± 0.022	3.148ThrAsp: 3.148 ± 0.097	3.383ThrGlu: 3.383 ± 0.067	3.337ThrPhe: 3.337 ± 0.062	3.872ThrGly: 3.872 ± 0.092	0.952ThrHis: 0.952 ± 0.037	5.457ThrIle: 5.457 ± 0.113	4.547ThrLys: 4.547 ± 0.066	5.153ThrLeu: 5.153 ± 0.078	0.929ThrMet: 0.929 ± 0.033	3.599ThrAsn: 3.599 ± 0.083	2.372ThrPro: 2.372 ± 0.11	1.646ThrGln: 1.646 ± 0.044	1.59ThrArg: 1.59 ± 0.037	4.099ThrSer: 4.099 ± 0.096	3.509ThrThr: 3.509 ± 0.143	3.538ThrVal: 3.538 ± 0.087	0.633ThrTrp: 0.633 ± 0.037	2.204ThrTyr: 2.204 ± 0.067	0.0ThrXaa: 0.0 ± 0.0
Val	3.991ValAla: 3.991 ± 0.076	0.523ValCys: 0.523 ± 0.037	3.334ValAsp: 3.334 ± 0.061	3.46ValGlu: 3.46 ± 0.066	3.454ValPhe: 3.454 ± 0.071	3.736ValGly: 3.736 ± 0.065	1.006ValHis: 1.006 ± 0.031	4.845ValIle: 4.845 ± 0.086	4.558ValLys: 4.558 ± 0.073	5.889ValLeu: 5.889 ± 0.086	1.182ValMet: 1.182 ± 0.039	3.542ValAsn: 3.542 ± 0.065	1.875ValPro: 1.875 ± 0.044	1.67ValGln: 1.67 ± 0.044	1.811ValArg: 1.811 ± 0.047	4.524ValSer: 4.524 ± 0.082	3.553ValThr: 3.553 ± 0.137	3.826ValVal: 3.826 ± 0.084	0.611ValTrp: 0.611 ± 0.029	2.12ValTyr: 2.12 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.54TrpAla: 0.54 ± 0.028	0.097TrpCys: 0.097 ± 0.01	0.581TrpAsp: 0.581 ± 0.027	0.614TrpGlu: 0.614 ± 0.023	0.611TrpPhe: 0.611 ± 0.026	0.667TrpGly: 0.667 ± 0.029	0.198TrpHis: 0.198 ± 0.015	0.793TrpIle: 0.793 ± 0.03	0.854TrpLys: 0.854 ± 0.036	0.932TrpLeu: 0.932 ± 0.036	0.312TrpMet: 0.312 ± 0.021	0.731TrpAsn: 0.731 ± 0.029	0.21TrpPro: 0.21 ± 0.017	0.382TrpGln: 0.382 ± 0.025	0.407TrpArg: 0.407 ± 0.023	0.737TrpSer: 0.737 ± 0.045	0.519TrpThr: 0.519 ± 0.026	0.563TrpVal: 0.563 ± 0.029	0.12TrpTrp: 0.12 ± 0.011	0.419TrpTyr: 0.419 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.171TyrAla: 2.171 ± 0.05	0.319TyrCys: 0.319 ± 0.021	1.983TyrAsp: 1.983 ± 0.05	1.994TyrGlu: 1.994 ± 0.052	2.458TyrPhe: 2.458 ± 0.066	2.352TyrGly: 2.352 ± 0.058	0.728TyrHis: 0.728 ± 0.029	2.761TyrIle: 2.761 ± 0.058	3.38TyrLys: 3.38 ± 0.063	3.608TyrLeu: 3.608 ± 0.067	0.675TyrMet: 0.675 ± 0.028	2.684TyrAsn: 2.684 ± 0.052	1.419TyrPro: 1.419 ± 0.035	1.565TyrGln: 1.565 ± 0.049	1.431TyrArg: 1.431 ± 0.036	2.564TyrSer: 2.564 ± 0.058	2.459TyrThr: 2.459 ± 0.094	1.949TyrVal: 1.949 ± 0.047	0.446TyrTrp: 0.446 ± 0.024	1.6TyrTyr: 1.6 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2741 proteins (952477 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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