Macaca nemestrina herpesvirus 7
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191S3V9|A0A191S3V9_9BETA Uracil-DNA glycosylase OS=Macaca nemestrina herpesvirus 7 OX=1846169 GN=U81 PE=3 SV=1
MM1 pKa = 6.23 QTNIYY6 pKa = 10.37 FCFFFNFSTSLYY18 pKa = 10.68 ALFNNSYY25 pKa = 10.47 YY26 pKa = 9.78 STLGKK31 pKa = 10.45 EE32 pKa = 4.79 CINAIKK38 pKa = 10.63 NCTQSEE44 pKa = 4.35 RR45 pKa = 11.84 PILLEE50 pKa = 5.52 PIDD53 pKa = 4.15 QAPTPNPDD61 pKa = 3.53 VISGLLYY68 pKa = 7.12 YY69 pKa = 9.99 TPYY72 pKa = 11.03 SFDD75 pKa = 3.52 EE76 pKa = 4.34 YY77 pKa = 11.08 QVLVDD82 pKa = 4.79 EE83 pKa = 5.05 EE84 pKa = 4.42 FLNTFYY90 pKa = 11.65 LLYY93 pKa = 10.75 NNPNQLHH100 pKa = 5.71 VLFSLIKK107 pKa = 9.77 DD108 pKa = 3.95 SKK110 pKa = 11.25 SKK112 pKa = 10.97 SSWLGFLNSFEE123 pKa = 4.81 KK124 pKa = 10.87 CFSDD128 pKa = 3.81 NTIITCRR135 pKa = 11.84 NQVCKK140 pKa = 9.96 TYY142 pKa = 10.52 SYY144 pKa = 11.45 EE145 pKa = 4.02 NLKK148 pKa = 8.55 YY149 pKa = 8.93 TPNIFVEE156 pKa = 4.43 NIIGFEE162 pKa = 4.06 FYY164 pKa = 10.35 ISEE167 pKa = 4.27 NNLDD171 pKa = 3.44 VSFNSSILIYY181 pKa = 10.17 LQNEE185 pKa = 3.66 EE186 pKa = 4.0 TRR188 pKa = 11.84 AKK190 pKa = 10.33 KK191 pKa = 9.53 IVKK194 pKa = 9.73 IMYY197 pKa = 9.53 EE198 pKa = 4.28 GINVFDD204 pKa = 4.72 ALLNSMRR211 pKa = 11.84 YY212 pKa = 9.4 FSQKK216 pKa = 9.96 FQFSFDD222 pKa = 3.67 FPLLRR227 pKa = 11.84 EE228 pKa = 4.08 MEE230 pKa = 4.58 SYY232 pKa = 11.62 NDD234 pKa = 3.8 LLPFRR239 pKa = 11.84 SEE241 pKa = 3.87 PSNLLIRR248 pKa = 11.84 THH250 pKa = 6.33
Molecular weight: 29.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.776
IPC2_protein 4.889
IPC_protein 4.774
Toseland 4.647
ProMoST 4.825
Dawson 4.711
Bjellqvist 4.863
Wikipedia 4.558
Rodwell 4.622
Grimsley 4.558
Solomon 4.711
Lehninger 4.66
Nozaki 4.825
DTASelect 4.94
Thurlkill 4.635
EMBOSS 4.584
Sillero 4.889
Patrickios 0.744
IPC_peptide 4.724
IPC2_peptide 4.889
IPC2.peptide.svr19 4.831
Protein with the highest isoelectric point:
>tr|A0A192XPA1|A0A192XPA1_9BETA Capsid scaffold protein OS=Macaca nemestrina herpesvirus 7 OX=1846169 GN=U53.5 PE=4 SV=1
MM1 pKa = 7.68 SLEE4 pKa = 4.18 HH5 pKa = 6.66 LPAIRR10 pKa = 11.84 KK11 pKa = 9.48 CIGQYY16 pKa = 9.19 NHH18 pKa = 6.03 LRR20 pKa = 11.84 IYY22 pKa = 10.87 KK23 pKa = 9.63 KK24 pKa = 10.42 ILMLKK29 pKa = 10.66 SNFTEE34 pKa = 4.42 LNFFLGNIFPHH45 pKa = 6.58 EE46 pKa = 4.05 LQTSKK51 pKa = 10.16 IHH53 pKa = 6.12 VFFEE57 pKa = 4.22 VTLGNRR63 pKa = 11.84 IPDD66 pKa = 4.28 CIIVLNHH73 pKa = 6.05 LNEE76 pKa = 3.89 NRR78 pKa = 11.84 VNEE81 pKa = 3.53 IHH83 pKa = 7.42 LYY85 pKa = 9.69 FFEE88 pKa = 5.1 FKK90 pKa = 8.42 TTFAKK95 pKa = 9.49 STLFSIQKK103 pKa = 8.31 NTTQKK108 pKa = 10.63 LQYY111 pKa = 10.46 LQGLKK116 pKa = 9.92 QLQEE120 pKa = 3.66 ATKK123 pKa = 10.42 FLEE126 pKa = 3.92 QYY128 pKa = 10.18 LIKK131 pKa = 10.96 NEE133 pKa = 4.3 TTCKK137 pKa = 9.4 ISPVICFFRR146 pKa = 11.84 QRR148 pKa = 11.84 GLKK151 pKa = 10.08 LDD153 pKa = 3.8 FVKK156 pKa = 10.3 TFVSKK161 pKa = 10.42 EE162 pKa = 4.06 LQLSLPFILNLLAQFEE178 pKa = 4.54 NDD180 pKa = 3.46 TVKK183 pKa = 10.88 SILSISDD190 pKa = 3.17 TAHH193 pKa = 6.78 FRR195 pKa = 11.84 RR196 pKa = 11.84 ACQKK200 pKa = 9.88 YY201 pKa = 8.52 SHH203 pKa = 6.29 LQRR206 pKa = 11.84 RR207 pKa = 11.84 RR208 pKa = 11.84 NSKK211 pKa = 9.21 TSTNRR216 pKa = 11.84 SNNTAKK222 pKa = 10.71 KK223 pKa = 9.97 KK224 pKa = 10.16 RR225 pKa = 11.84 RR226 pKa = 11.84 NPKK229 pKa = 9.65 KK230 pKa = 10.49
Molecular weight: 27.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.16
IPC2_protein 9.414
IPC_protein 9.37
Toseland 10.35
ProMoST 9.897
Dawson 10.467
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 11.082
Grimsley 10.496
Solomon 10.482
Lehninger 10.467
Nozaki 10.35
DTASelect 10.058
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.379
Patrickios 10.789
IPC_peptide 10.496
IPC2_peptide 8.726
IPC2.peptide.svr19 8.38
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
83
0
83
39259
54
2056
473.0
54.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.584 ± 0.135
2.455 ± 0.164
4.791 ± 0.145
6.106 ± 0.197
6.371 ± 0.198
3.169 ± 0.172
2.394 ± 0.08
8.559 ± 0.244
7.496 ± 0.236
10.479 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.198 ± 0.084
7.181 ± 0.197
3.505 ± 0.152
3.747 ± 0.15
3.691 ± 0.209
7.996 ± 0.248
6.218 ± 0.183
5.24 ± 0.155
0.769 ± 0.059
4.053 ± 0.123
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here