Corchorus yellow vein virus - [Hoa Binh]
Average proteome isoelectric point is 8.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q645G8|Q645G8_9GEMI Nuclear shuttle protein OS=Corchorus yellow vein virus - [Hoa Binh] OX=293284 GN=BV1 PE=3 SV=1
MM1 pKa = 7.74 EE2 pKa = 4.83 SQLAQAPSSFNYY14 pKa = 9.76 IEE16 pKa = 4.09 SHH18 pKa = 6.17 RR19 pKa = 11.84 DD20 pKa = 3.25 EE21 pKa = 4.44 YY22 pKa = 11.48 RR23 pKa = 11.84 LTHH26 pKa = 7.07 DD27 pKa = 3.36 LTEE30 pKa = 4.44 IVLQFPSTAEE40 pKa = 3.5 QWGATVRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 CMKK52 pKa = 9.72 IDD54 pKa = 3.27 HH55 pKa = 6.48 CVIEE59 pKa = 4.31 YY60 pKa = 9.79 RR61 pKa = 11.84 QQVPINATGSVIVEE75 pKa = 3.82 IHH77 pKa = 7.1 DD78 pKa = 4.31 KK79 pKa = 11.26 RR80 pKa = 11.84 MTDD83 pKa = 3.41 NEE85 pKa = 4.46 SLQASYY91 pKa = 9.77 TFPIRR96 pKa = 11.84 CNIDD100 pKa = 2.86 LHH102 pKa = 6.12 YY103 pKa = 10.57 FSASFFSLKK112 pKa = 10.83 DD113 pKa = 3.77 PIPWKK118 pKa = 10.22 LYY120 pKa = 10.12 YY121 pKa = 9.87 RR122 pKa = 11.84 VSDD125 pKa = 3.88 TNVHH129 pKa = 5.9 QGTHH133 pKa = 4.64 FAKK136 pKa = 10.88 FKK138 pKa = 11.0 GKK140 pKa = 10.75 LKK142 pKa = 10.36 MSTAKK147 pKa = 10.51 HH148 pKa = 5.34 SVDD151 pKa = 3.66 VIFRR155 pKa = 11.84 SPTVKK160 pKa = 10.15 ILSKK164 pKa = 10.78 QFTGKK169 pKa = 10.37 DD170 pKa = 2.79 IDD172 pKa = 4.26 FSHH175 pKa = 7.02 VDD177 pKa = 3.27 YY178 pKa = 11.46 GKK180 pKa = 10.29 VEE182 pKa = 4.18 RR183 pKa = 11.84 KK184 pKa = 9.14 LVKK187 pKa = 10.4 CEE189 pKa = 3.68 SSSRR193 pKa = 11.84 LGLHH197 pKa = 5.88 SAIEE201 pKa = 4.02 LRR203 pKa = 11.84 PGEE206 pKa = 4.2 SWATRR211 pKa = 11.84 SCIGPDD217 pKa = 3.12 TMEE220 pKa = 4.52 TEE222 pKa = 4.46 SEE224 pKa = 4.3 VHH226 pKa = 5.96 NRR228 pKa = 11.84 NHH230 pKa = 7.86 PYY232 pKa = 10.35 RR233 pKa = 11.84 EE234 pKa = 4.15 LNRR237 pKa = 11.84 LSTSMLDD244 pKa = 3.84 PGDD247 pKa = 3.96 SASMIGASRR256 pKa = 11.84 AEE258 pKa = 4.24 SNITLTRR265 pKa = 11.84 AQLQEE270 pKa = 4.35 LVSSTVEE277 pKa = 3.45 HH278 pKa = 6.95 CINRR282 pKa = 11.84 NCTPHH287 pKa = 5.59 QAKK290 pKa = 9.86 PLL292 pKa = 3.84
Molecular weight: 33.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.972
IPC2_protein 7.22
IPC_protein 7.205
Toseland 7.059
ProMoST 7.717
Dawson 7.849
Bjellqvist 8.126
Wikipedia 7.761
Rodwell 7.849
Grimsley 7.146
Solomon 7.922
Lehninger 7.936
Nozaki 8.404
DTASelect 7.995
Thurlkill 8.053
EMBOSS 8.097
Sillero 8.331
Patrickios 4.38
IPC_peptide 7.922
IPC2_peptide 7.468
IPC2.peptide.svr19 7.266
Protein with the highest isoelectric point:
>tr|Q645H3|Q645H3_9GEMI Capsid protein OS=Corchorus yellow vein virus - [Hoa Binh] OX=293284 GN=AV1 PE=3 SV=1
MM1 pKa = 7.98 PKK3 pKa = 9.65 RR4 pKa = 11.84 DD5 pKa = 3.82 APWRR9 pKa = 11.84 LMAGTSKK16 pKa = 10.64 VSRR19 pKa = 11.84 SSNYY23 pKa = 9.02 SPRR26 pKa = 11.84 GGVSDD31 pKa = 3.63 SGSYY35 pKa = 10.25 LPRR38 pKa = 11.84 RR39 pKa = 11.84 FSRR42 pKa = 11.84 ASLWANRR49 pKa = 11.84 PMYY52 pKa = 9.88 RR53 pKa = 11.84 KK54 pKa = 8.83 PRR56 pKa = 11.84 IYY58 pKa = 9.41 RR59 pKa = 11.84 TYY61 pKa = 10.72 RR62 pKa = 11.84 SPDD65 pKa = 3.24 VPKK68 pKa = 10.81 GCEE71 pKa = 4.39 GPCKK75 pKa = 10.23 VQSFEE80 pKa = 3.84 QRR82 pKa = 11.84 HH83 pKa = 6.27 DD84 pKa = 3.18 IAHH87 pKa = 6.05 TGKK90 pKa = 10.57 VICLSDD96 pKa = 3.31 VTRR99 pKa = 11.84 GNGITHH105 pKa = 6.78 RR106 pKa = 11.84 VGKK109 pKa = 9.44 RR110 pKa = 11.84 FCVKK114 pKa = 9.92 SVYY117 pKa = 10.16 ILGKK121 pKa = 9.37 IWMDD125 pKa = 3.32 EE126 pKa = 4.0 NIKK129 pKa = 10.42 VKK131 pKa = 10.63 NHH133 pKa = 5.61 TNSVMFWLVRR143 pKa = 11.84 DD144 pKa = 3.83 RR145 pKa = 11.84 RR146 pKa = 11.84 PFGSPMDD153 pKa = 4.16 FGQVFNMYY161 pKa = 10.66 DD162 pKa = 3.86 NEE164 pKa = 4.34 PSTATVKK171 pKa = 10.81 NDD173 pKa = 2.84 LRR175 pKa = 11.84 DD176 pKa = 3.6 RR177 pKa = 11.84 YY178 pKa = 10.31 QVMHH182 pKa = 7.01 RR183 pKa = 11.84 FSAKK187 pKa = 8.35 VTGGQYY193 pKa = 11.1 ASNEE197 pKa = 3.84 QALVRR202 pKa = 11.84 RR203 pKa = 11.84 FWKK206 pKa = 10.4 VNNHH210 pKa = 4.48 VVYY213 pKa = 10.66 NHH215 pKa = 5.84 QEE217 pKa = 3.45 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 9.86 ATLKK247 pKa = 9.47 IRR249 pKa = 11.84 IYY251 pKa = 10.59 FYY253 pKa = 11.32 DD254 pKa = 4.06 SISNN258 pKa = 3.66
Molecular weight: 29.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.545
IPC_protein 9.823
Toseland 9.999
ProMoST 9.794
Dawson 10.248
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.526
Grimsley 10.35
Solomon 10.277
Lehninger 10.233
Nozaki 10.043
DTASelect 9.97
Thurlkill 10.087
EMBOSS 10.423
Sillero 10.175
Patrickios 9.589
IPC_peptide 10.277
IPC2_peptide 8.902
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1529
96
359
218.4
25.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.494 ± 0.305
2.224 ± 0.151
4.709 ± 0.238
4.709 ± 0.591
4.513 ± 0.418
4.578 ± 0.484
3.597 ± 0.477
5.821 ± 0.681
5.298 ± 0.563
6.475 ± 0.373
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.42 ± 0.466
5.755 ± 0.746
5.69 ± 0.421
3.728 ± 0.43
7.848 ± 1.046
9.614 ± 0.546
6.148 ± 0.423
5.559 ± 0.556
1.439 ± 0.273
4.382 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here