Microbacterium phage ArMaWen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Tinytimothyvirus; unclassified Tinytimothyvirus

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IP36|A0A3T0IP36_9CAUD Uncharacterized protein OS=Microbacterium phage ArMaWen OX=2500786 GN=27 PE=4 SV=1
MM1 pKa = 7.29STTLDD6 pKa = 3.44QCGTTPDD13 pKa = 3.76TLYY16 pKa = 7.55PTPDD20 pKa = 3.45NCWEE24 pKa = 4.02QVGTNVIVHH33 pKa = 5.88YY34 pKa = 10.49KK35 pKa = 10.68GEE37 pKa = 4.75DD38 pKa = 3.39YY39 pKa = 10.58THH41 pKa = 7.51AILGDD46 pKa = 3.44ATVAMVQAQDD56 pKa = 4.57CPDD59 pKa = 4.25LYY61 pKa = 10.58ATCNTTSIVWAYY73 pKa = 9.13PPSAPVQTPLAQTGVDD89 pKa = 3.41PGLVVALVVGAIIVSGVGSWLLAAGGKK116 pKa = 9.16RR117 pKa = 11.84RR118 pKa = 11.84NKK120 pKa = 10.59GEE122 pKa = 3.81

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9RAC9|A0A3Q9RAC9_9CAUD Uncharacterized protein OS=Microbacterium phage ArMaWen OX=2500786 GN=30 PE=4 SV=2
MM1 pKa = 7.64SDD3 pKa = 2.91KK4 pKa = 11.25GFIITNINEE13 pKa = 4.07GMRR16 pKa = 11.84DD17 pKa = 3.88TIAFITIEE25 pKa = 4.01ARR27 pKa = 11.84LKK29 pKa = 9.55VAIRR33 pKa = 11.84LGRR36 pKa = 11.84TDD38 pKa = 3.23TQALHH43 pKa = 6.25SARR46 pKa = 11.84KK47 pKa = 8.65LAAKK51 pKa = 9.86YY52 pKa = 9.71KK53 pKa = 10.21YY54 pKa = 8.97PGNVKK59 pKa = 8.63TMKK62 pKa = 9.97QALRR66 pKa = 11.84MCEE69 pKa = 3.67AAIKK73 pKa = 10.53AMRR76 pKa = 11.84PDD78 pKa = 4.2DD79 pKa = 4.01EE80 pKa = 4.3QDD82 pKa = 2.88RR83 pKa = 4.13

Molecular weight: 9.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

17065

43

4483

316.0

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.686 ± 0.399

0.475 ± 0.137

6.774 ± 0.224

6.587 ± 0.33

3.428 ± 0.158

7.589 ± 0.328

1.623 ± 0.202

4.635 ± 0.202

4.424 ± 0.349

8.368 ± 0.358

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.147

4.489 ± 0.288

4.729 ± 0.276

4.582 ± 0.235

6.001 ± 0.386

6.012 ± 0.217

6.44 ± 0.411

6.505 ± 0.359

1.723 ± 0.119

3.217 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski