Amino acid dipepetide frequency for Microbacterium phage ArMaWen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.307AlaAla: 12.307 ± 1.321
0.469AlaCys: 0.469 ± 0.196
5.45AlaAsp: 5.45 ± 0.571
5.098AlaGlu: 5.098 ± 0.583
3.282AlaPhe: 3.282 ± 0.399
6.564AlaGly: 6.564 ± 0.996
1.699AlaHis: 1.699 ± 0.398
4.219AlaIle: 4.219 ± 0.524
3.575AlaLys: 3.575 ± 0.682
8.497AlaLeu: 8.497 ± 0.709
2.403AlaMet: 2.403 ± 0.497
4.571AlaAsn: 4.571 ± 0.6
5.567AlaPro: 5.567 ± 1.194
3.868AlaGln: 3.868 ± 0.453
6.388AlaArg: 6.388 ± 0.656
6.329AlaSer: 6.329 ± 0.69
6.036AlaThr: 6.036 ± 0.819
5.684AlaVal: 5.684 ± 0.485
1.817AlaTrp: 1.817 ± 0.268
2.989AlaTyr: 2.989 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
0.234CysAla: 0.234 ± 0.142
0.0CysCys: 0.0 ± 0.0
0.059CysAsp: 0.059 ± 0.059
0.586CysGlu: 0.586 ± 0.274
0.234CysPhe: 0.234 ± 0.151
0.469CysGly: 0.469 ± 0.219
0.059CysHis: 0.059 ± 0.056
0.176CysIle: 0.176 ± 0.124
0.176CysLys: 0.176 ± 0.127
0.352CysLeu: 0.352 ± 0.185
0.176CysMet: 0.176 ± 0.117
0.117CysAsn: 0.117 ± 0.09
0.41CysPro: 0.41 ± 0.211
0.234CysGln: 0.234 ± 0.169
0.176CysArg: 0.176 ± 0.112
0.352CysSer: 0.352 ± 0.169
0.293CysThr: 0.293 ± 0.182
0.293CysVal: 0.293 ± 0.158
0.059CysTrp: 0.059 ± 0.065
0.293CysTyr: 0.293 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
7.794AspAla: 7.794 ± 0.769
0.527AspCys: 0.527 ± 0.225
4.981AspAsp: 4.981 ± 0.566
4.688AspGlu: 4.688 ± 0.546
2.051AspPhe: 2.051 ± 0.336
6.915AspGly: 6.915 ± 0.731
1.172AspHis: 1.172 ± 0.215
3.516AspIle: 3.516 ± 0.418
3.165AspLys: 3.165 ± 0.625
4.747AspLeu: 4.747 ± 0.572
1.817AspMet: 1.817 ± 0.27
2.872AspAsn: 2.872 ± 0.415
3.106AspPro: 3.106 ± 0.491
3.165AspGln: 3.165 ± 0.401
2.872AspArg: 2.872 ± 0.479
3.633AspSer: 3.633 ± 0.291
3.34AspThr: 3.34 ± 0.594
3.868AspVal: 3.868 ± 0.739
1.699AspTrp: 1.699 ± 0.278
1.817AspTyr: 1.817 ± 0.306
0.0AspXaa: 0.0 ± 0.0
Glu
5.684GluAla: 5.684 ± 0.507
0.117GluCys: 0.117 ± 0.089
4.278GluAsp: 4.278 ± 0.59
4.278GluGlu: 4.278 ± 0.649
2.813GluPhe: 2.813 ± 0.43
4.395GluGly: 4.395 ± 0.461
1.172GluHis: 1.172 ± 0.336
3.575GluIle: 3.575 ± 0.553
3.751GluLys: 3.751 ± 0.665
6.739GluLeu: 6.739 ± 0.786
1.582GluMet: 1.582 ± 0.287
3.223GluAsn: 3.223 ± 0.504
2.168GluPro: 2.168 ± 0.589
2.754GluGln: 2.754 ± 0.441
4.454GluArg: 4.454 ± 0.642
3.633GluSer: 3.633 ± 0.448
3.282GluThr: 3.282 ± 0.429
3.868GluVal: 3.868 ± 0.403
1.406GluTrp: 1.406 ± 0.303
2.696GluTyr: 2.696 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
2.051PheAla: 2.051 ± 0.399
0.41PheCys: 0.41 ± 0.209
2.579PheAsp: 2.579 ± 0.436
3.047PheGlu: 3.047 ± 0.635
1.113PhePhe: 1.113 ± 0.212
2.461PheGly: 2.461 ± 0.291
0.645PheHis: 0.645 ± 0.244
1.289PheIle: 1.289 ± 0.236
1.875PheLys: 1.875 ± 0.284
2.52PheLeu: 2.52 ± 0.364
0.996PheMet: 0.996 ± 0.24
1.524PheAsn: 1.524 ± 0.379
1.641PhePro: 1.641 ± 0.267
1.289PheGln: 1.289 ± 0.281
2.461PheArg: 2.461 ± 0.446
2.11PheSer: 2.11 ± 0.312
2.11PheThr: 2.11 ± 0.446
2.813PheVal: 2.813 ± 0.487
0.352PheTrp: 0.352 ± 0.148
0.996PheTyr: 0.996 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
7.384GlyAla: 7.384 ± 1.096
0.059GlyCys: 0.059 ± 0.062
4.454GlyAsp: 4.454 ± 0.395
5.333GlyGlu: 5.333 ± 0.581
2.579GlyPhe: 2.579 ± 0.37
6.974GlyGly: 6.974 ± 1.047
1.172GlyHis: 1.172 ± 0.232
4.278GlyIle: 4.278 ± 0.634
3.047GlyLys: 3.047 ± 0.495
5.977GlyLeu: 5.977 ± 0.671
2.286GlyMet: 2.286 ± 0.414
2.461GlyAsn: 2.461 ± 0.477
2.11GlyPro: 2.11 ± 0.346
3.516GlyGln: 3.516 ± 0.385
4.571GlyArg: 4.571 ± 0.604
4.864GlySer: 4.864 ± 0.638
5.157GlyThr: 5.157 ± 0.733
5.509GlyVal: 5.509 ± 0.523
1.055GlyTrp: 1.055 ± 0.25
3.106GlyTyr: 3.106 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
0.938HisAla: 0.938 ± 0.268
0.176HisCys: 0.176 ± 0.131
1.231HisAsp: 1.231 ± 0.28
1.348HisGlu: 1.348 ± 0.363
0.352HisPhe: 0.352 ± 0.125
0.938HisGly: 0.938 ± 0.274
0.234HisHis: 0.234 ± 0.17
0.82HisIle: 0.82 ± 0.181
1.231HisLys: 1.231 ± 0.45
1.289HisLeu: 1.289 ± 0.261
0.352HisMet: 0.352 ± 0.178
0.469HisAsn: 0.469 ± 0.156
1.231HisPro: 1.231 ± 0.241
0.82HisGln: 0.82 ± 0.178
1.113HisArg: 1.113 ± 0.247
0.703HisSer: 0.703 ± 0.168
0.645HisThr: 0.645 ± 0.258
1.582HisVal: 1.582 ± 0.359
0.176HisTrp: 0.176 ± 0.07
0.586HisTyr: 0.586 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
4.337IleAla: 4.337 ± 0.561
0.176IleCys: 0.176 ± 0.118
4.747IleAsp: 4.747 ± 0.603
3.282IleGlu: 3.282 ± 0.533
1.113IlePhe: 1.113 ± 0.235
2.286IleGly: 2.286 ± 0.557
0.352IleHis: 0.352 ± 0.112
2.403IleIle: 2.403 ± 0.47
2.989IleLys: 2.989 ± 0.472
3.809IleLeu: 3.809 ± 0.459
0.996IleMet: 0.996 ± 0.257
2.344IleAsn: 2.344 ± 0.504
2.168IlePro: 2.168 ± 0.335
2.051IleGln: 2.051 ± 0.328
2.696IleArg: 2.696 ± 0.464
3.047IleSer: 3.047 ± 0.39
2.637IleThr: 2.637 ± 0.363
2.93IleVal: 2.93 ± 0.607
0.762IleTrp: 0.762 ± 0.211
1.231IleTyr: 1.231 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
4.161LysAla: 4.161 ± 0.573
0.293LysCys: 0.293 ± 0.157
2.989LysAsp: 2.989 ± 0.403
3.165LysGlu: 3.165 ± 0.466
1.172LysPhe: 1.172 ± 0.239
2.579LysGly: 2.579 ± 0.387
0.586LysHis: 0.586 ± 0.183
1.524LysIle: 1.524 ± 0.23
2.051LysLys: 2.051 ± 0.457
3.985LysLeu: 3.985 ± 0.613
0.82LysMet: 0.82 ± 0.185
2.872LysAsn: 2.872 ± 0.328
2.11LysPro: 2.11 ± 0.465
1.699LysGln: 1.699 ± 0.29
2.52LysArg: 2.52 ± 0.696
2.168LysSer: 2.168 ± 0.52
3.633LysThr: 3.633 ± 0.439
3.047LysVal: 3.047 ± 0.464
0.82LysTrp: 0.82 ± 0.288
2.051LysTyr: 2.051 ± 0.464
0.0LysXaa: 0.0 ± 0.0
Leu
7.677LeuAla: 7.677 ± 0.736
0.586LeuCys: 0.586 ± 0.266
5.567LeuAsp: 5.567 ± 0.556
5.216LeuGlu: 5.216 ± 0.55
2.813LeuPhe: 2.813 ± 0.451
6.388LeuGly: 6.388 ± 0.789
1.406LeuHis: 1.406 ± 0.24
3.692LeuIle: 3.692 ± 0.763
3.516LeuLys: 3.516 ± 0.528
6.681LeuLeu: 6.681 ± 0.606
3.106LeuMet: 3.106 ± 0.521
3.282LeuAsn: 3.282 ± 0.44
3.985LeuPro: 3.985 ± 0.568
4.044LeuGln: 4.044 ± 0.739
5.86LeuArg: 5.86 ± 0.62
5.216LeuSer: 5.216 ± 0.816
5.274LeuThr: 5.274 ± 0.491
5.333LeuVal: 5.333 ± 0.711
1.231LeuTrp: 1.231 ± 0.324
2.813LeuTyr: 2.813 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
3.165MetAla: 3.165 ± 0.357
0.176MetCys: 0.176 ± 0.137
1.524MetAsp: 1.524 ± 0.205
1.172MetGlu: 1.172 ± 0.362
1.289MetPhe: 1.289 ± 0.254
1.934MetGly: 1.934 ± 0.737
0.469MetHis: 0.469 ± 0.145
0.996MetIle: 0.996 ± 0.225
1.641MetLys: 1.641 ± 0.246
1.992MetLeu: 1.992 ± 0.306
0.586MetMet: 0.586 ± 0.162
1.231MetAsn: 1.231 ± 0.227
1.406MetPro: 1.406 ± 0.255
1.231MetGln: 1.231 ± 0.256
1.524MetArg: 1.524 ± 0.438
2.52MetSer: 2.52 ± 0.48
1.992MetThr: 1.992 ± 0.362
1.231MetVal: 1.231 ± 0.291
0.352MetTrp: 0.352 ± 0.149
0.703MetTyr: 0.703 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
4.102AsnAla: 4.102 ± 0.699
0.293AsnCys: 0.293 ± 0.146
2.52AsnAsp: 2.52 ± 0.507
2.344AsnGlu: 2.344 ± 0.292
1.465AsnPhe: 1.465 ± 0.283
4.805AsnGly: 4.805 ± 0.641
0.645AsnHis: 0.645 ± 0.288
1.817AsnIle: 1.817 ± 0.341
2.11AsnLys: 2.11 ± 0.487
3.809AsnLeu: 3.809 ± 0.764
1.055AsnMet: 1.055 ± 0.234
1.758AsnAsn: 1.758 ± 0.353
2.872AsnPro: 2.872 ± 0.668
2.403AsnGln: 2.403 ± 0.346
1.699AsnArg: 1.699 ± 0.444
2.286AsnSer: 2.286 ± 0.473
3.399AsnThr: 3.399 ± 0.529
3.633AsnVal: 3.633 ± 0.517
0.469AsnTrp: 0.469 ± 0.144
1.406AsnTyr: 1.406 ± 0.263
0.0AsnXaa: 0.0 ± 0.0
Pro
4.864ProAla: 4.864 ± 0.77
0.117ProCys: 0.117 ± 0.083
3.516ProAsp: 3.516 ± 0.4
4.102ProGlu: 4.102 ± 0.679
1.231ProPhe: 1.231 ± 0.224
3.575ProGly: 3.575 ± 0.643
0.527ProHis: 0.527 ± 0.263
2.696ProIle: 2.696 ± 0.445
1.641ProLys: 1.641 ± 0.475
3.985ProLeu: 3.985 ± 0.526
0.938ProMet: 0.938 ± 0.22
2.813ProAsn: 2.813 ± 0.311
1.875ProPro: 1.875 ± 0.314
1.465ProGln: 1.465 ± 0.292
2.11ProArg: 2.11 ± 0.358
2.872ProSer: 2.872 ± 0.393
3.282ProThr: 3.282 ± 0.524
3.751ProVal: 3.751 ± 0.459
0.762ProTrp: 0.762 ± 0.207
1.172ProTyr: 1.172 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
5.919GlnAla: 5.919 ± 0.632
0.234GlnCys: 0.234 ± 0.133
2.989GlnAsp: 2.989 ± 0.409
2.696GlnGlu: 2.696 ± 0.45
1.524GlnPhe: 1.524 ± 0.22
2.93GlnGly: 2.93 ± 0.392
0.82GlnHis: 0.82 ± 0.166
1.934GlnIle: 1.934 ± 0.249
1.348GlnLys: 1.348 ± 0.301
4.161GlnLeu: 4.161 ± 0.415
1.524GlnMet: 1.524 ± 0.288
1.992GlnAsn: 1.992 ± 0.395
1.992GlnPro: 1.992 ± 0.315
2.696GlnGln: 2.696 ± 0.57
3.399GlnArg: 3.399 ± 0.399
2.286GlnSer: 2.286 ± 0.354
2.696GlnThr: 2.696 ± 0.373
2.286GlnVal: 2.286 ± 0.36
0.879GlnTrp: 0.879 ± 0.256
1.524GlnTyr: 1.524 ± 0.318
0.0GlnXaa: 0.0 ± 0.0
Arg
4.63ArgAla: 4.63 ± 0.701
0.059ArgCys: 0.059 ± 0.067
3.751ArgAsp: 3.751 ± 0.437
5.216ArgGlu: 5.216 ± 0.538
2.461ArgPhe: 2.461 ± 0.587
4.337ArgGly: 4.337 ± 0.637
0.82ArgHis: 0.82 ± 0.226
2.344ArgIle: 2.344 ± 0.395
2.286ArgLys: 2.286 ± 0.403
5.391ArgLeu: 5.391 ± 0.707
1.758ArgMet: 1.758 ± 0.274
2.813ArgAsn: 2.813 ± 0.506
2.754ArgPro: 2.754 ± 0.374
2.989ArgGln: 2.989 ± 0.458
4.805ArgArg: 4.805 ± 0.843
3.399ArgSer: 3.399 ± 0.744
3.809ArgThr: 3.809 ± 0.56
4.219ArgVal: 4.219 ± 0.474
0.703ArgTrp: 0.703 ± 0.2
1.465ArgTyr: 1.465 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
5.684SerAla: 5.684 ± 0.548
0.117SerCys: 0.117 ± 0.093
4.395SerAsp: 4.395 ± 0.53
2.813SerGlu: 2.813 ± 0.338
3.047SerPhe: 3.047 ± 0.414
5.567SerGly: 5.567 ± 0.623
0.996SerHis: 0.996 ± 0.233
2.696SerIle: 2.696 ± 0.386
2.579SerLys: 2.579 ± 0.259
4.102SerLeu: 4.102 ± 0.48
1.875SerMet: 1.875 ± 0.333
3.165SerAsn: 3.165 ± 0.404
2.696SerPro: 2.696 ± 0.392
2.93SerGln: 2.93 ± 0.439
3.165SerArg: 3.165 ± 0.343
3.516SerSer: 3.516 ± 0.493
3.868SerThr: 3.868 ± 0.637
3.751SerVal: 3.751 ± 0.397
1.348SerTrp: 1.348 ± 0.246
1.817SerTyr: 1.817 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
5.684ThrAla: 5.684 ± 0.805
0.117ThrCys: 0.117 ± 0.099
4.454ThrAsp: 4.454 ± 0.436
4.395ThrGlu: 4.395 ± 0.456
1.875ThrPhe: 1.875 ± 0.358
5.626ThrGly: 5.626 ± 0.828
0.996ThrHis: 0.996 ± 0.293
3.165ThrIle: 3.165 ± 0.453
2.051ThrLys: 2.051 ± 0.365
5.86ThrLeu: 5.86 ± 0.7
1.699ThrMet: 1.699 ± 0.303
2.52ThrAsn: 2.52 ± 0.405
3.282ThrPro: 3.282 ± 0.407
2.637ThrGln: 2.637 ± 0.409
3.516ThrArg: 3.516 ± 0.421
4.161ThrSer: 4.161 ± 0.518
5.509ThrThr: 5.509 ± 0.762
3.516ThrVal: 3.516 ± 0.442
1.641ThrTrp: 1.641 ± 0.35
1.699ThrTyr: 1.699 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
6.036ValAla: 6.036 ± 0.677
0.352ValCys: 0.352 ± 0.171
4.219ValAsp: 4.219 ± 0.433
4.161ValGlu: 4.161 ± 0.448
2.168ValPhe: 2.168 ± 0.316
4.395ValGly: 4.395 ± 0.602
1.582ValHis: 1.582 ± 0.383
2.989ValIle: 2.989 ± 0.351
2.754ValLys: 2.754 ± 0.427
5.802ValLeu: 5.802 ± 0.614
1.465ValMet: 1.465 ± 0.279
2.461ValAsn: 2.461 ± 0.424
4.044ValPro: 4.044 ± 0.638
3.458ValGln: 3.458 ± 0.325
4.219ValArg: 4.219 ± 0.528
3.633ValSer: 3.633 ± 0.387
4.395ValThr: 4.395 ± 0.582
3.516ValVal: 3.516 ± 0.415
1.172ValTrp: 1.172 ± 0.208
2.227ValTyr: 2.227 ± 0.336
0.0ValXaa: 0.0 ± 0.0
Trp
1.231TrpAla: 1.231 ± 0.22
0.059TrpCys: 0.059 ± 0.067
1.641TrpAsp: 1.641 ± 0.288
0.938TrpGlu: 0.938 ± 0.243
0.586TrpPhe: 0.586 ± 0.172
1.289TrpGly: 1.289 ± 0.407
0.234TrpHis: 0.234 ± 0.115
0.703TrpIle: 0.703 ± 0.205
0.82TrpLys: 0.82 ± 0.277
1.641TrpLeu: 1.641 ± 0.36
0.527TrpMet: 0.527 ± 0.198
0.703TrpAsn: 0.703 ± 0.251
0.469TrpPro: 0.469 ± 0.167
0.762TrpGln: 0.762 ± 0.2
0.762TrpArg: 0.762 ± 0.177
1.348TrpSer: 1.348 ± 0.339
1.289TrpThr: 1.289 ± 0.266
1.465TrpVal: 1.465 ± 0.326
0.234TrpTrp: 0.234 ± 0.13
0.527TrpTyr: 0.527 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.989TyrAla: 2.989 ± 0.391
0.41TyrCys: 0.41 ± 0.219
2.403TyrAsp: 2.403 ± 0.316
1.992TyrGlu: 1.992 ± 0.317
1.113TyrPhe: 1.113 ± 0.263
1.465TyrGly: 1.465 ± 0.369
0.645TyrHis: 0.645 ± 0.265
1.524TyrIle: 1.524 ± 0.224
1.641TyrLys: 1.641 ± 0.258
2.344TyrLeu: 2.344 ± 0.384
1.113TyrMet: 1.113 ± 0.215
1.699TyrAsn: 1.699 ± 0.432
1.406TyrPro: 1.406 ± 0.45
1.817TyrGln: 1.817 ± 0.233
1.699TyrArg: 1.699 ± 0.335
2.051TyrSer: 2.051 ± 0.306
1.758TyrThr: 1.758 ± 0.286
2.754TyrVal: 2.754 ± 0.363
0.293TyrTrp: 0.293 ± 0.142
1.055TyrTyr: 1.055 ± 0.261
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (17065 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski