Nitrosococcus halophilus (strain Nc4)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Nitrosococcus; Nitrosococcus halophilus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5BWV0|D5BWV0_NITHN 4-hydroxy-tetrahydrodipicolinate synthase OS=Nitrosococcus halophilus (strain Nc4) OX=472759 GN=dapA PE=3 SV=1
MM1 pKa = 7.32NNKK4 pKa = 9.89KK5 pKa = 10.18IIATIATVVPILFTADD21 pKa = 2.96ISAQSSDD28 pKa = 3.45QPLYY32 pKa = 10.95APYY35 pKa = 10.26EE36 pKa = 4.17GWPPCAVMEE45 pKa = 4.47AAGEE49 pKa = 4.17TMPDD53 pKa = 3.45GQVVTGVICRR63 pKa = 11.84SNPFIPDD70 pKa = 3.24SDD72 pKa = 4.02NDD74 pKa = 4.19GVPDD78 pKa = 5.25DD79 pKa = 4.71TDD81 pKa = 3.35QCPGTPEE88 pKa = 3.96GVEE91 pKa = 4.05VDD93 pKa = 3.86TVGCPLDD100 pKa = 3.91NDD102 pKa = 3.66NDD104 pKa = 4.24GVPDD108 pKa = 4.43YY109 pKa = 11.33LDD111 pKa = 3.41QCPDD115 pKa = 3.35TPVGVAVNSEE125 pKa = 4.04GCPLDD130 pKa = 3.56SDD132 pKa = 4.33GDD134 pKa = 4.19GVPDD138 pKa = 5.11YY139 pKa = 11.33LDD141 pKa = 3.44QCPDD145 pKa = 3.54TPPGVAVNSEE155 pKa = 4.06GCPLDD160 pKa = 3.61SDD162 pKa = 4.38GDD164 pKa = 4.3GVTDD168 pKa = 6.4DD169 pKa = 5.41VDD171 pKa = 3.59QCPDD175 pKa = 3.08TPAGVEE181 pKa = 3.91VDD183 pKa = 3.75EE184 pKa = 4.93VGCPEE189 pKa = 4.04PVVLEE194 pKa = 4.36GGVHH198 pKa = 6.41FAFDD202 pKa = 3.82SAKK205 pKa = 9.29LTPEE209 pKa = 3.7ARR211 pKa = 11.84DD212 pKa = 3.64ILDD215 pKa = 3.42EE216 pKa = 4.31TADD219 pKa = 3.76SLRR222 pKa = 11.84THH224 pKa = 7.59PDD226 pKa = 2.97LEE228 pKa = 4.53VTIEE232 pKa = 3.86GHH234 pKa = 5.53TDD236 pKa = 2.84SVGNADD242 pKa = 3.51YY243 pKa = 11.19NKK245 pKa = 10.3RR246 pKa = 11.84LSQLRR251 pKa = 11.84AEE253 pKa = 4.36AAMNYY258 pKa = 9.51LVSQGVDD265 pKa = 3.19QSRR268 pKa = 11.84LEE270 pKa = 3.86AVGYY274 pKa = 10.99GEE276 pKa = 4.59AQPIASNKK284 pKa = 7.59TKK286 pKa = 10.43EE287 pKa = 3.79GRR289 pKa = 11.84AQNRR293 pKa = 11.84RR294 pKa = 11.84VEE296 pKa = 4.36LHH298 pKa = 5.19THH300 pKa = 6.44DD301 pKa = 3.94

Molecular weight: 31.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5C444|D5C444_NITHN Multicopper oxidase type 3 OS=Nitrosococcus halophilus (strain Nc4) OX=472759 GN=Nhal_0010 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.02NLKK11 pKa = 9.99RR12 pKa = 11.84KK13 pKa = 7.29RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TQGGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3770

0

3770

1165698

23

3608

309.2

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.303 ± 0.037

1.0 ± 0.015

4.699 ± 0.027

6.9 ± 0.039

3.897 ± 0.03

7.613 ± 0.039

2.453 ± 0.022

5.564 ± 0.029

4.062 ± 0.033

11.381 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.016

3.055 ± 0.027

5.123 ± 0.028

4.54 ± 0.034

6.715 ± 0.03

5.449 ± 0.027

4.995 ± 0.032

6.758 ± 0.033

1.484 ± 0.018

2.855 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski