Flectobacillus sp. BAB-3569

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Flectobacillus; unclassified Flectobacillus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A267T4J3|A0A267T4J3_9BACT Ornithine--oxo-acid aminotransferase OS=Flectobacillus sp. BAB-3569 OX=1509483 GN=rocD PE=3 SV=1
MM1 pKa = 7.68NILQKK6 pKa = 10.39IVILLVCLTLVSVKK20 pKa = 10.09IQAQDD25 pKa = 3.2VSINIINQPSTVTQGSTLGRR45 pKa = 11.84VTVDD49 pKa = 2.6ICNNDD54 pKa = 3.17GGSRR58 pKa = 11.84SAAANRR64 pKa = 11.84LRR66 pKa = 11.84PLITLPSSLVGSVIPITTTGWTVLPIDD93 pKa = 4.09INNPTTLRR101 pKa = 11.84FEE103 pKa = 4.24NTVAIAPGEE112 pKa = 4.03CSQIVIGYY120 pKa = 7.34TGVNIGGPLTVTGTLGFNGPQTTGNLTGNDD150 pKa = 3.45NSTTSITVITGDD162 pKa = 3.54TDD164 pKa = 3.97GDD166 pKa = 4.09GDD168 pKa = 4.8PDD170 pKa = 3.5ITDD173 pKa = 4.08PQPTDD178 pKa = 3.04PCAWGTGQVLANTTTAWRR196 pKa = 11.84TADD199 pKa = 3.7CDD201 pKa = 3.85GDD203 pKa = 4.25GVTNYY208 pKa = 10.36KK209 pKa = 10.12EE210 pKa = 4.07ATGTDD215 pKa = 4.16DD216 pKa = 6.2DD217 pKa = 5.17PLTLGDD223 pKa = 3.9NTNPNDD229 pKa = 3.62GCSYY233 pKa = 10.94NKK235 pKa = 9.61VDD237 pKa = 3.69QVIGNVSASWKK248 pKa = 9.17TLDD251 pKa = 4.56CDD253 pKa = 4.38KK254 pKa = 11.21DD255 pKa = 3.92GNPNEE260 pKa = 4.45TDD262 pKa = 3.94LNPQVATAVNDD273 pKa = 3.36VLTAPFGLTSTVSVLSNDD291 pKa = 3.36DD292 pKa = 3.94FLPVASNEE300 pKa = 3.61ITRR303 pKa = 11.84IGGNASGTVAFAPTTGVMSYY323 pKa = 9.35TPSATEE329 pKa = 3.6PGGPNVTVVYY339 pKa = 7.71QVCNTLVTPNVCASATVTISVPAAGDD365 pKa = 3.31QDD367 pKa = 4.59GDD369 pKa = 3.86GDD371 pKa = 4.8PDD373 pKa = 3.69NTDD376 pKa = 3.45PQPTNSCVWGIGQVLANTSTTWRR399 pKa = 11.84NADD402 pKa = 3.47CDD404 pKa = 4.03GDD406 pKa = 4.45GVTNYY411 pKa = 10.62NEE413 pKa = 3.83ATGADD418 pKa = 4.14GNPATLADD426 pKa = 3.81NTDD429 pKa = 3.83PLSACSLNLGQVXIRR444 pKa = 11.84FVLLLDD450 pKa = 3.46YY451 pKa = 11.35SGFLRR456 pKa = 11.84NFANSLDD463 pKa = 3.88TPLILLSGQIQYY475 pKa = 11.52GKK477 pKa = 9.52MFFGG481 pKa = 4.32

Molecular weight: 49.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A267T511|A0A267T511_9BACT NAGPA domain-containing protein OS=Flectobacillus sp. BAB-3569 OX=1509483 GN=BWI92_20980 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.25KK3 pKa = 9.95VEE5 pKa = 4.0YY6 pKa = 10.32LNRR9 pKa = 11.84RR10 pKa = 11.84MRR12 pKa = 11.84LKK14 pKa = 10.33IVSDD18 pKa = 3.97DD19 pKa = 3.65FLTISTLEE27 pKa = 3.84YY28 pKa = 10.29RR29 pKa = 11.84VGSFLGIGIWRR40 pKa = 11.84RR41 pKa = 11.84VGDD44 pKa = 3.81RR45 pKa = 11.84VEE47 pKa = 4.76LGSNVQLQQIVLSIQFTRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 9.95NKK69 pKa = 9.91LEE71 pKa = 3.78RR72 pKa = 11.84RR73 pKa = 11.84ASLTQIAEE81 pKa = 4.49KK82 pKa = 10.38LCKK85 pKa = 9.28TATPP89 pKa = 4.06

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5084

0

5084

1755997

26

3834

345.4

38.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.918 ± 0.036

0.838 ± 0.011

5.082 ± 0.023

6.035 ± 0.045

4.962 ± 0.025

6.611 ± 0.036

1.791 ± 0.018

7.137 ± 0.03

7.206 ± 0.038

9.519 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.018

5.602 ± 0.036

3.751 ± 0.02

4.147 ± 0.024

3.678 ± 0.022

6.755 ± 0.038

5.946 ± 0.043

6.435 ± 0.031

1.293 ± 0.015

4.093 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski