Amino acid dipepetide frequency for Flectobacillus sp. BAB-3569

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.387AlaAla: 4.387 ± 0.077
0.675AlaCys: 0.675 ± 0.02
3.518AlaAsp: 3.518 ± 0.051
4.251AlaGlu: 4.251 ± 0.064
3.347AlaPhe: 3.347 ± 0.045
4.745AlaGly: 4.745 ± 0.069
1.124AlaHis: 1.124 ± 0.031
5.081AlaIle: 5.081 ± 0.063
4.905AlaLys: 4.905 ± 0.073
6.709AlaLeu: 6.709 ± 0.077
1.572AlaMet: 1.572 ± 0.034
3.849AlaAsn: 3.849 ± 0.052
2.367AlaPro: 2.367 ± 0.044
3.408AlaGln: 3.408 ± 0.049
2.231AlaArg: 2.231 ± 0.032
4.965AlaSer: 4.965 ± 0.067
4.213AlaThr: 4.213 ± 0.075
4.253AlaVal: 4.253 ± 0.059
0.863AlaTrp: 0.863 ± 0.025
2.717AlaTyr: 2.717 ± 0.046
0.001AlaXaa: 0.001 ± 0.001
Cys
0.479CysAla: 0.479 ± 0.017
0.113CysCys: 0.113 ± 0.009
0.434CysAsp: 0.434 ± 0.017
0.502CysGlu: 0.502 ± 0.022
0.447CysPhe: 0.447 ± 0.014
0.613CysGly: 0.613 ± 0.021
0.218CysHis: 0.218 ± 0.012
0.587CysIle: 0.587 ± 0.02
0.507CysLys: 0.507 ± 0.021
0.811CysLeu: 0.811 ± 0.024
0.185CysMet: 0.185 ± 0.011
0.4CysAsn: 0.4 ± 0.016
0.362CysPro: 0.362 ± 0.015
0.363CysGln: 0.363 ± 0.014
0.289CysArg: 0.289 ± 0.013
0.627CysSer: 0.627 ± 0.022
0.483CysThr: 0.483 ± 0.019
0.519CysVal: 0.519 ± 0.017
0.1CysTrp: 0.1 ± 0.008
0.338CysTyr: 0.338 ± 0.014
0.001CysXaa: 0.001 ± 0.001
Asp
3.687AspAla: 3.687 ± 0.048
0.427AspCys: 0.427 ± 0.017
2.259AspAsp: 2.259 ± 0.039
3.243AspGlu: 3.243 ± 0.057
3.269AspPhe: 3.269 ± 0.049
3.763AspGly: 3.763 ± 0.073
0.738AspHis: 0.738 ± 0.021
3.745AspIle: 3.745 ± 0.049
3.724AspLys: 3.724 ± 0.053
5.028AspLeu: 5.028 ± 0.063
1.047AspMet: 1.047 ± 0.02
2.719AspAsn: 2.719 ± 0.046
1.756AspPro: 1.756 ± 0.038
1.429AspGln: 1.429 ± 0.027
1.759AspArg: 1.759 ± 0.031
3.105AspSer: 3.105 ± 0.043
2.761AspThr: 2.761 ± 0.046
3.05AspVal: 3.05 ± 0.044
0.854AspTrp: 0.854 ± 0.025
2.455AspTyr: 2.455 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
4.368GluAla: 4.368 ± 0.066
0.35GluCys: 0.35 ± 0.016
2.809GluAsp: 2.809 ± 0.04
4.116GluGlu: 4.116 ± 0.086
2.519GluPhe: 2.519 ± 0.039
3.623GluGly: 3.623 ± 0.047
1.088GluHis: 1.088 ± 0.028
4.746GluIle: 4.746 ± 0.059
5.425GluLys: 5.425 ± 0.083
5.833GluLeu: 5.833 ± 0.065
1.433GluMet: 1.433 ± 0.03
3.654GluAsn: 3.654 ± 0.051
1.681GluPro: 1.681 ± 0.035
2.621GluGln: 2.621 ± 0.052
2.497GluArg: 2.497 ± 0.04
3.377GluSer: 3.377 ± 0.048
2.985GluThr: 2.985 ± 0.041
4.167GluVal: 4.167 ± 0.055
0.706GluTrp: 0.706 ± 0.021
2.352GluTyr: 2.352 ± 0.042
0.001GluXaa: 0.001 ± 0.001
Phe
3.192PheAla: 3.192 ± 0.041
0.538PheCys: 0.538 ± 0.018
2.976PheAsp: 2.976 ± 0.039
3.349PheGlu: 3.349 ± 0.049
2.428PhePhe: 2.428 ± 0.047
3.478PheGly: 3.478 ± 0.05
0.841PheHis: 0.841 ± 0.025
3.081PheIle: 3.081 ± 0.054
3.149PheLys: 3.149 ± 0.044
4.553PheLeu: 4.553 ± 0.059
1.129PheMet: 1.129 ± 0.028
2.644PheAsn: 2.644 ± 0.043
1.714PhePro: 1.714 ± 0.033
1.866PheGln: 1.866 ± 0.036
1.883PheArg: 1.883 ± 0.033
3.862PheSer: 3.862 ± 0.056
2.921PheThr: 2.921 ± 0.042
3.23PheVal: 3.23 ± 0.044
0.732PheTrp: 0.732 ± 0.024
2.055PheTyr: 2.055 ± 0.039
0.002PheXaa: 0.002 ± 0.001
Gly
4.331GlyAla: 4.331 ± 0.068
0.651GlyCys: 0.651 ± 0.024
3.114GlyAsp: 3.114 ± 0.05
3.248GlyGlu: 3.248 ± 0.047
3.636GlyPhe: 3.636 ± 0.056
4.717GlyGly: 4.717 ± 0.083
1.115GlyHis: 1.115 ± 0.026
5.333GlyIle: 5.333 ± 0.057
5.394GlyLys: 5.394 ± 0.064
6.264GlyLeu: 6.264 ± 0.08
1.599GlyMet: 1.599 ± 0.033
3.579GlyAsn: 3.579 ± 0.064
1.29GlyPro: 1.29 ± 0.032
2.435GlyGln: 2.435 ± 0.042
2.383GlyArg: 2.383 ± 0.036
4.159GlySer: 4.159 ± 0.068
4.237GlyThr: 4.237 ± 0.068
4.67GlyVal: 4.67 ± 0.055
0.935GlyTrp: 0.935 ± 0.023
3.015GlyTyr: 3.015 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
1.064HisAla: 1.064 ± 0.026
0.181HisCys: 0.181 ± 0.01
0.802HisAsp: 0.802 ± 0.022
1.081HisGlu: 1.081 ± 0.03
1.154HisPhe: 1.154 ± 0.026
1.068HisGly: 1.068 ± 0.026
0.525HisHis: 0.525 ± 0.021
1.363HisIle: 1.363 ± 0.032
1.071HisLys: 1.071 ± 0.027
1.838HisLeu: 1.838 ± 0.037
0.211HisMet: 0.211 ± 0.01
0.854HisAsn: 0.854 ± 0.021
0.964HisPro: 0.964 ± 0.023
0.874HisGln: 0.874 ± 0.025
0.785HisArg: 0.785 ± 0.021
1.035HisSer: 1.035 ± 0.024
0.999HisThr: 0.999 ± 0.026
0.899HisVal: 0.899 ± 0.026
0.324HisTrp: 0.324 ± 0.015
0.814HisTyr: 0.814 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.347IleAla: 5.347 ± 0.055
0.731IleCys: 0.731 ± 0.022
4.054IleAsp: 4.054 ± 0.052
4.655IleGlu: 4.655 ± 0.068
3.176IlePhe: 3.176 ± 0.056
4.744IleGly: 4.744 ± 0.055
1.316IleHis: 1.316 ± 0.031
4.599IleIle: 4.599 ± 0.064
4.776IleLys: 4.776 ± 0.06
6.638IleLeu: 6.638 ± 0.078
1.288IleMet: 1.288 ± 0.03
3.846IleAsn: 3.846 ± 0.051
3.191IlePro: 3.191 ± 0.04
3.064IleGln: 3.064 ± 0.046
2.795IleArg: 2.795 ± 0.041
5.252IleSer: 5.252 ± 0.061
4.217IleThr: 4.217 ± 0.054
4.329IleVal: 4.329 ± 0.058
0.786IleTrp: 0.786 ± 0.021
2.563IleTyr: 2.563 ± 0.038
0.001IleXaa: 0.001 ± 0.001
Lys
5.371LysAla: 5.371 ± 0.072
0.372LysCys: 0.372 ± 0.015
4.052LysAsp: 4.052 ± 0.056
4.756LysGlu: 4.756 ± 0.073
2.685LysPhe: 2.685 ± 0.045
4.682LysGly: 4.682 ± 0.055
1.292LysHis: 1.292 ± 0.031
5.353LysIle: 5.353 ± 0.058
5.448LysLys: 5.448 ± 0.067
6.208LysLeu: 6.208 ± 0.069
1.923LysMet: 1.923 ± 0.03
4.317LysAsn: 4.317 ± 0.062
2.744LysPro: 2.744 ± 0.044
2.735LysGln: 2.735 ± 0.042
2.654LysArg: 2.654 ± 0.042
4.456LysSer: 4.456 ± 0.058
4.098LysThr: 4.098 ± 0.048
5.105LysVal: 5.105 ± 0.064
0.81LysTrp: 0.81 ± 0.023
2.996LysTyr: 2.996 ± 0.042
0.001LysXaa: 0.001 ± 0.001
Leu
6.682LeuAla: 6.682 ± 0.077
0.843LeuCys: 0.843 ± 0.021
4.842LeuAsp: 4.842 ± 0.051
5.689LeuGlu: 5.689 ± 0.062
4.506LeuPhe: 4.506 ± 0.057
6.389LeuGly: 6.389 ± 0.07
1.654LeuHis: 1.654 ± 0.032
6.175LeuIle: 6.175 ± 0.078
7.142LeuLys: 7.142 ± 0.078
9.285LeuLeu: 9.285 ± 0.096
2.213LeuMet: 2.213 ± 0.043
5.08LeuAsn: 5.08 ± 0.058
4.068LeuPro: 4.068 ± 0.051
3.826LeuGln: 3.826 ± 0.052
3.599LeuArg: 3.599 ± 0.05
7.363LeuSer: 7.363 ± 0.075
5.592LeuThr: 5.592 ± 0.066
5.832LeuVal: 5.832 ± 0.059
1.081LeuTrp: 1.081 ± 0.028
3.331LeuTyr: 3.331 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
1.669MetAla: 1.669 ± 0.031
0.141MetCys: 0.141 ± 0.01
1.01MetAsp: 1.01 ± 0.024
1.138MetGlu: 1.138 ± 0.026
0.829MetPhe: 0.829 ± 0.022
1.525MetGly: 1.525 ± 0.029
0.333MetHis: 0.333 ± 0.013
1.488MetIle: 1.488 ± 0.035
2.03MetLys: 2.03 ± 0.034
2.139MetLeu: 2.139 ± 0.034
0.634MetMet: 0.634 ± 0.019
1.271MetAsn: 1.271 ± 0.027
1.005MetPro: 1.005 ± 0.022
0.855MetGln: 0.855 ± 0.023
0.876MetArg: 0.876 ± 0.022
1.497MetSer: 1.497 ± 0.031
1.309MetThr: 1.309 ± 0.028
1.35MetVal: 1.35 ± 0.029
0.196MetTrp: 0.196 ± 0.011
0.709MetTyr: 0.709 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.909AsnAla: 3.909 ± 0.055
0.413AsnCys: 0.413 ± 0.017
2.645AsnAsp: 2.645 ± 0.047
2.88AsnGlu: 2.88 ± 0.047
2.792AsnPhe: 2.792 ± 0.049
3.813AsnGly: 3.813 ± 0.058
1.114AsnHis: 1.114 ± 0.026
4.044AsnIle: 4.044 ± 0.054
3.315AsnLys: 3.315 ± 0.049
5.329AsnLeu: 5.329 ± 0.064
1.1AsnMet: 1.1 ± 0.022
3.139AsnAsn: 3.139 ± 0.057
3.059AsnPro: 3.059 ± 0.042
2.624AsnGln: 2.624 ± 0.046
2.163AsnArg: 2.163 ± 0.037
3.464AsnSer: 3.464 ± 0.046
3.72AsnThr: 3.72 ± 0.054
3.134AsnVal: 3.134 ± 0.048
0.78AsnTrp: 0.78 ± 0.024
2.581AsnTyr: 2.581 ± 0.045
0.001AsnXaa: 0.001 ± 0.001
Pro
2.706ProAla: 2.706 ± 0.053
0.226ProCys: 0.226 ± 0.012
2.151ProAsp: 2.151 ± 0.037
2.768ProGlu: 2.768 ± 0.052
1.884ProPhe: 1.884 ± 0.035
1.498ProGly: 1.498 ± 0.033
0.702ProHis: 0.702 ± 0.023
2.805ProIle: 2.805 ± 0.039
2.587ProLys: 2.587 ± 0.041
3.271ProLeu: 3.271 ± 0.045
0.815ProMet: 0.815 ± 0.021
2.508ProAsn: 2.508 ± 0.042
0.949ProPro: 0.949 ± 0.028
1.591ProGln: 1.591 ± 0.031
1.122ProArg: 1.122 ± 0.027
2.878ProSer: 2.878 ± 0.052
2.436ProThr: 2.436 ± 0.046
2.67ProVal: 2.67 ± 0.041
0.428ProTrp: 0.428 ± 0.016
1.513ProTyr: 1.513 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
2.929GlnAla: 2.929 ± 0.047
0.226GlnCys: 0.226 ± 0.013
1.802GlnAsp: 1.802 ± 0.032
2.612GlnGlu: 2.612 ± 0.048
1.827GlnPhe: 1.827 ± 0.032
2.24GlnGly: 2.24 ± 0.043
0.753GlnHis: 0.753 ± 0.02
2.991GlnIle: 2.991 ± 0.038
3.615GlnLys: 3.615 ± 0.048
3.936GlnLeu: 3.936 ± 0.054
0.985GlnMet: 0.985 ± 0.022
2.634GlnAsn: 2.634 ± 0.041
1.384GlnPro: 1.384 ± 0.026
2.036GlnGln: 2.036 ± 0.04
1.515GlnArg: 1.515 ± 0.031
2.568GlnSer: 2.568 ± 0.034
2.507GlnThr: 2.507 ± 0.048
2.684GlnVal: 2.684 ± 0.044
0.527GlnTrp: 0.527 ± 0.02
1.699GlnTyr: 1.699 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.287ArgAla: 2.287 ± 0.041
0.252ArgCys: 0.252 ± 0.014
1.745ArgAsp: 1.745 ± 0.033
2.173ArgGlu: 2.173 ± 0.043
2.056ArgPhe: 2.056 ± 0.037
2.17ArgGly: 2.17 ± 0.037
0.653ArgHis: 0.653 ± 0.02
2.845ArgIle: 2.845 ± 0.041
2.782ArgLys: 2.782 ± 0.051
3.669ArgLeu: 3.669 ± 0.054
0.95ArgMet: 0.95 ± 0.024
2.136ArgAsn: 2.136 ± 0.039
1.332ArgPro: 1.332 ± 0.031
1.524ArgGln: 1.524 ± 0.031
1.498ArgArg: 1.498 ± 0.031
1.964ArgSer: 1.964 ± 0.035
2.054ArgThr: 2.054 ± 0.034
2.486ArgVal: 2.486 ± 0.041
0.544ArgTrp: 0.544 ± 0.019
1.662ArgTyr: 1.662 ± 0.031
0.001ArgXaa: 0.001 ± 0.001
Ser
4.456SerAla: 4.456 ± 0.069
0.617SerCys: 0.617 ± 0.02
3.325SerAsp: 3.325 ± 0.051
3.732SerGlu: 3.732 ± 0.049
3.736SerPhe: 3.736 ± 0.052
4.685SerGly: 4.685 ± 0.065
1.163SerHis: 1.163 ± 0.026
5.032SerIle: 5.032 ± 0.059
4.484SerLys: 4.484 ± 0.056
6.587SerLeu: 6.587 ± 0.071
1.35SerMet: 1.35 ± 0.029
3.73SerAsn: 3.73 ± 0.052
2.563SerPro: 2.563 ± 0.036
2.731SerGln: 2.731 ± 0.043
2.207SerArg: 2.207 ± 0.038
4.739SerSer: 4.739 ± 0.069
4.263SerThr: 4.263 ± 0.059
4.415SerVal: 4.415 ± 0.058
0.854SerTrp: 0.854 ± 0.024
2.879SerTyr: 2.879 ± 0.049
0.001SerXaa: 0.001 ± 0.001
Thr
4.153ThrAla: 4.153 ± 0.072
0.454ThrCys: 0.454 ± 0.024
3.045ThrAsp: 3.045 ± 0.047
3.169ThrGlu: 3.169 ± 0.046
3.066ThrPhe: 3.066 ± 0.044
4.444ThrGly: 4.444 ± 0.08
1.068ThrHis: 1.068 ± 0.032
4.378ThrIle: 4.378 ± 0.058
3.725ThrLys: 3.725 ± 0.049
5.747ThrLeu: 5.747 ± 0.062
1.025ThrMet: 1.025 ± 0.025
3.164ThrAsn: 3.164 ± 0.054
2.761ThrPro: 2.761 ± 0.04
2.425ThrGln: 2.425 ± 0.037
1.887ThrArg: 1.887 ± 0.031
4.006ThrSer: 4.006 ± 0.073
3.899ThrThr: 3.899 ± 0.091
3.885ThrVal: 3.885 ± 0.064
0.743ThrTrp: 0.743 ± 0.022
2.409ThrTyr: 2.409 ± 0.042
0.001ThrXaa: 0.001 ± 0.001
Val
4.621ValAla: 4.621 ± 0.052
0.647ValCys: 0.647 ± 0.023
3.386ValAsp: 3.386 ± 0.047
3.943ValGlu: 3.943 ± 0.053
3.183ValPhe: 3.183 ± 0.041
4.363ValGly: 4.363 ± 0.058
1.051ValHis: 1.051 ± 0.029
4.429ValIle: 4.429 ± 0.054
4.581ValLys: 4.581 ± 0.052
6.019ValLeu: 6.019 ± 0.065
1.399ValMet: 1.399 ± 0.032
3.511ValAsn: 3.511 ± 0.057
2.356ValPro: 2.356 ± 0.039
2.246ValGln: 2.246 ± 0.034
2.348ValArg: 2.348 ± 0.041
4.711ValSer: 4.711 ± 0.061
3.715ValThr: 3.715 ± 0.059
4.587ValVal: 4.587 ± 0.056
0.823ValTrp: 0.823 ± 0.023
2.428ValTyr: 2.428 ± 0.039
0.002ValXaa: 0.002 ± 0.001
Trp
0.838TrpAla: 0.838 ± 0.023
0.117TrpCys: 0.117 ± 0.008
0.653TrpAsp: 0.653 ± 0.019
0.773TrpGlu: 0.773 ± 0.023
0.621TrpPhe: 0.621 ± 0.018
0.935TrpGly: 0.935 ± 0.024
0.277TrpHis: 0.277 ± 0.013
0.875TrpIle: 0.875 ± 0.024
0.899TrpLys: 0.899 ± 0.025
1.244TrpLeu: 1.244 ± 0.026
0.368TrpMet: 0.368 ± 0.015
0.768TrpAsn: 0.768 ± 0.026
0.266TrpPro: 0.266 ± 0.014
0.655TrpGln: 0.655 ± 0.021
0.525TrpArg: 0.525 ± 0.017
0.846TrpSer: 0.846 ± 0.025
0.688TrpThr: 0.688 ± 0.022
0.825TrpVal: 0.825 ± 0.023
0.218TrpTrp: 0.218 ± 0.013
0.544TrpTyr: 0.544 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.703TyrAla: 2.703 ± 0.047
0.401TyrCys: 0.401 ± 0.015
2.195TyrAsp: 2.195 ± 0.037
2.274TyrGlu: 2.274 ± 0.038
2.461TyrPhe: 2.461 ± 0.042
2.61TyrGly: 2.61 ± 0.041
0.882TyrHis: 0.882 ± 0.023
2.419TyrIle: 2.419 ± 0.036
2.494TyrLys: 2.494 ± 0.043
4.081TyrLeu: 4.081 ± 0.056
0.778TyrMet: 0.778 ± 0.022
2.215TyrAsn: 2.215 ± 0.046
1.696TyrPro: 1.696 ± 0.031
2.161TyrGln: 2.161 ± 0.038
1.768TyrArg: 1.768 ± 0.03
2.673TyrSer: 2.673 ± 0.036
2.359TyrThr: 2.359 ± 0.04
2.261TyrVal: 2.261 ± 0.042
0.629TyrTrp: 0.629 ± 0.021
1.868TyrTyr: 1.868 ± 0.04
0.001TyrXaa: 0.001 ± 0.001
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.001XaaGlu: 0.001 ± 0.001
0.001XaaPhe: 0.001 ± 0.001
0.001XaaGly: 0.001 ± 0.001
0.0XaaHis: 0.0 ± 0.0
0.001XaaIle: 0.001 ± 0.001
0.001XaaLys: 0.001 ± 0.001
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.001XaaAsn: 0.001 ± 0.001
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.001
0.002XaaSer: 0.002 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.001XaaVal: 0.001 ± 0.001
0.0XaaTrp: 0.0 ± 0.0
0.001XaaTyr: 0.001 ± 0.001
0.005XaaXaa: 0.005 ± 0.002
Statistics based on 5084 proteins (1755997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski