Devosia sp. M6-77

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Devosiaceae; Devosia; unclassified Devosia

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3943 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A447IBU0|A0A447IBU0_9RHIZ Phosphate regulon transcriptional regulatory protein PhoB OS=Devosia sp. M6-77 OX=2490941 GN=phoB PE=4 SV=1
MM1 pKa = 7.85LDD3 pKa = 3.45DD4 pKa = 3.71QLAIGTEE11 pKa = 3.71IDD13 pKa = 3.27AGLVGQEE20 pKa = 4.07QGSKK24 pKa = 10.74AVFACGDD31 pKa = 3.43IVVEE35 pKa = 4.14HH36 pKa = 5.59QHH38 pKa = 7.03VIDD41 pKa = 4.1EE42 pKa = 4.31QVAACCIALDD52 pKa = 3.65GDD54 pKa = 3.93RR55 pKa = 11.84PVNADD60 pKa = 3.45GLADD64 pKa = 3.95GDD66 pKa = 4.22GSEE69 pKa = 4.69GPPANEE75 pKa = 4.08ARR77 pKa = 11.84GAYY80 pKa = 9.06CQQ82 pKa = 3.58

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A447IAH9|A0A447IAH9_9RHIZ Putative amino-acid metabolite efflux pump OS=Devosia sp. M6-77 OX=2490941 GN=eamA_1 PE=4 SV=1
MM1 pKa = 7.02IRR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.37VSKK8 pKa = 10.51LANPQLIALAGVLLINWLLFPNFFRR33 pKa = 11.84ITWQDD38 pKa = 2.46GRR40 pKa = 11.84LFGSLIDD47 pKa = 3.74VLNRR51 pKa = 11.84GAPVAILAIGMAGVIATKK69 pKa = 10.53GVDD72 pKa = 3.68LSVGAVMAVAGAVAATLVVAGYY94 pKa = 6.69PAPVAVTAALVVGLLCGLWNGFLVAVLDD122 pKa = 3.73IQPIVATLVLMVAGRR137 pKa = 11.84GIAQLITQGFIVTFNDD153 pKa = 3.31PVLIFIGTGSFLGLPMATVIALVLMVLVTLLVRR186 pKa = 11.84RR187 pKa = 11.84TAIGLFIEE195 pKa = 4.41AVGVNRR201 pKa = 11.84AAASLAGIRR210 pKa = 11.84SRR212 pKa = 11.84MLLMLVYY219 pKa = 10.3SLSGLCAAIAGIIVAGDD236 pKa = 3.27IRR238 pKa = 11.84GADD241 pKa = 3.59ANNAGLWLEE250 pKa = 4.35LDD252 pKa = 4.62AILAVVIGGTSLLGGRR268 pKa = 11.84FSVPLAVVGALIIQAMNTGILVSGFPPEE296 pKa = 3.82FNLIVKK302 pKa = 10.15AGLIFIVLLIQSPLASRR319 pKa = 11.84LAPVRR324 pKa = 11.84LRR326 pKa = 11.84PVAKK330 pKa = 10.16GAQKK334 pKa = 10.91

Molecular weight: 34.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3943

0

3943

1228497

29

2825

311.6

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.468 ± 0.051

0.673 ± 0.01

5.757 ± 0.034

5.494 ± 0.04

3.841 ± 0.024

8.557 ± 0.035

1.976 ± 0.02

5.514 ± 0.03

3.083 ± 0.032

10.373 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.016

2.795 ± 0.024

5.041 ± 0.03

3.303 ± 0.024

6.439 ± 0.038

5.445 ± 0.026

5.463 ± 0.027

7.578 ± 0.034

1.317 ± 0.017

2.288 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski