Candidatus Sneabacter namystus
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 583 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C0UJ36|A0A5C0UJ36_9RICK 30S ribosomal protein S19 OS=Candidatus Sneabacter namystus OX=2601646 GN=rpsS PE=3 SV=1
MM1 pKa = 7.65 SGIPITFIYY10 pKa = 9.99 PDD12 pKa = 3.76 GTQKK16 pKa = 10.12 IVNAQPGLSVLEE28 pKa = 4.21 VAKK31 pKa = 10.73 QHH33 pKa = 6.86 DD34 pKa = 4.11 IPIEE38 pKa = 4.19 GACGGGLACSTCHH51 pKa = 6.67 VIVEE55 pKa = 4.35 SQEE58 pKa = 4.87 AYY60 pKa = 10.56 DD61 pKa = 3.77 SLKK64 pKa = 10.95 APSEE68 pKa = 4.25 EE69 pKa = 4.16 EE70 pKa = 3.89 EE71 pKa = 5.08 DD72 pKa = 3.75 MLDD75 pKa = 3.38 LAFGLEE81 pKa = 4.19 EE82 pKa = 4.09 TSRR85 pKa = 11.84 LCCQILVDD93 pKa = 4.58 EE94 pKa = 4.78 SLSGIKK100 pKa = 10.05 FKK102 pKa = 10.88 IPSCSRR108 pKa = 11.84 NLL110 pKa = 3.47
Molecular weight: 11.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.081
IPC2_protein 4.406
IPC_protein 4.279
Toseland 4.126
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.088
Rodwell 4.113
Grimsley 4.037
Solomon 4.215
Lehninger 4.164
Nozaki 4.342
DTASelect 4.457
Thurlkill 4.139
EMBOSS 4.101
Sillero 4.393
Patrickios 1.977
IPC_peptide 4.215
IPC2_peptide 4.38
IPC2.peptide.svr19 4.295
Protein with the highest isoelectric point:
>tr|A0A5C0UHK3|A0A5C0UHK3_9RICK FtsQ-type POTRA domain-containing protein OS=Candidatus Sneabacter namystus OX=2601646 GN=FZC37_00900 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.44 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTVGGMLILQRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.78 GRR39 pKa = 11.84 VKK41 pKa = 10.69 LSAA44 pKa = 3.97
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
583
0
583
187933
42
1385
322.4
36.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.393 ± 0.105
1.77 ± 0.048
5.196 ± 0.083
5.784 ± 0.113
4.551 ± 0.082
5.65 ± 0.094
2.201 ± 0.039
8.298 ± 0.083
8.169 ± 0.104
9.914 ± 0.129
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.316 ± 0.044
4.943 ± 0.084
3.026 ± 0.045
3.446 ± 0.057
3.981 ± 0.065
8.106 ± 0.089
4.992 ± 0.075
7.222 ± 0.115
0.747 ± 0.031
3.296 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here