CRESS virus sp. ctxk12
Average proteome isoelectric point is 7.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W7X5|A0A5Q2W7X5_9VIRU Rep protein OS=CRESS virus sp. ctxk12 OX=2656692 PE=4 SV=1
MM1 pKa = 7.67 PGRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 WTWTYY11 pKa = 11.27 NNEE14 pKa = 4.33 ALNHH18 pKa = 5.72 ATTIEE23 pKa = 4.01 QIEE26 pKa = 4.35 WGDD29 pKa = 4.02 KK30 pKa = 9.88 IAADD34 pKa = 4.11 LTRR37 pKa = 11.84 VPDD40 pKa = 3.93 MQWMFSLEE48 pKa = 4.02 RR49 pKa = 11.84 GKK51 pKa = 10.73 SGNIHH56 pKa = 5.73 LQGYY60 pKa = 8.98 SKK62 pKa = 10.81 LPRR65 pKa = 11.84 AMSLAAMKK73 pKa = 10.15 IVLPGAHH80 pKa = 6.98 LLASRR85 pKa = 11.84 GSTAQNKK92 pKa = 9.21 AYY94 pKa = 10.17 CGKK97 pKa = 10.24 SDD99 pKa = 3.44 THH101 pKa = 7.35 IAGPWQSEE109 pKa = 4.06 EE110 pKa = 4.94 FIDD113 pKa = 5.12 DD114 pKa = 3.86 DD115 pKa = 4.22 EE116 pKa = 4.29 EE117 pKa = 5.19 AKK119 pKa = 10.81 EE120 pKa = 4.94 KK121 pKa = 10.92 EE122 pKa = 4.11 DD123 pKa = 4.4 LDD125 pKa = 4.15 AEE127 pKa = 4.33 KK128 pKa = 10.3 EE129 pKa = 3.94 AAAQAVIKK137 pKa = 10.31 CAEE140 pKa = 4.31 EE141 pKa = 3.89 GHH143 pKa = 6.66 LVQDD147 pKa = 3.58 MFKK150 pKa = 10.71 LLKK153 pKa = 10.33 DD154 pKa = 3.07 KK155 pKa = 10.03 RR156 pKa = 11.84 TRR158 pKa = 11.84 GQTIHH163 pKa = 6.58 MLPALRR169 pKa = 11.84 LASTGLPPPIIARR182 pKa = 11.84 GPLDD186 pKa = 3.46 IEE188 pKa = 4.29 LHH190 pKa = 6.31 FGDD193 pKa = 4.1 TGLGKK198 pKa = 8.66 TFHH201 pKa = 6.19 VRR203 pKa = 11.84 RR204 pKa = 11.84 KK205 pKa = 10.49 YY206 pKa = 9.96 PDD208 pKa = 3.47 SYY210 pKa = 11.7 LKK212 pKa = 10.67 DD213 pKa = 3.55 FHH215 pKa = 6.96 GKK217 pKa = 9.03 RR218 pKa = 11.84 DD219 pKa = 3.42 PWFDD223 pKa = 3.81 GYY225 pKa = 11.09 CGQTCLILDD234 pKa = 4.28 DD235 pKa = 5.16 FNGTGCPINTLKK247 pKa = 10.88 RR248 pKa = 11.84 LLDD251 pKa = 4.23 CYY253 pKa = 10.49 PCRR256 pKa = 11.84 LQIKK260 pKa = 10.43 GGTTWAAWTTAAITTQKK277 pKa = 11.09 DD278 pKa = 3.18 PIYY281 pKa = 9.25 WYY283 pKa = 9.65 PRR285 pKa = 11.84 EE286 pKa = 4.38 DD287 pKa = 3.5 EE288 pKa = 4.52 ADD290 pKa = 3.44 RR291 pKa = 11.84 KK292 pKa = 10.86 AIFRR296 pKa = 11.84 RR297 pKa = 11.84 IARR300 pKa = 11.84 VYY302 pKa = 9.13 QWSADD307 pKa = 3.13 GSIRR311 pKa = 11.84 WRR313 pKa = 11.84 PGLDD317 pKa = 3.14 HH318 pKa = 7.13 FEE320 pKa = 4.22 HH321 pKa = 7.35 RR322 pKa = 11.84 GMAWTVVDD330 pKa = 4.44 LGGPEE335 pKa = 3.85 GGEE338 pKa = 3.8 ARR340 pKa = 11.84 RR341 pKa = 11.84 DD342 pKa = 3.3 EE343 pKa = 4.77 DD344 pKa = 3.51 SAEE347 pKa = 4.1 FTSGQSDD354 pKa = 3.5 GGSWSRR360 pKa = 11.84 GGDD363 pKa = 3.21 GGGGGGRR370 pKa = 11.84 EE371 pKa = 3.95 RR372 pKa = 11.84 QRR374 pKa = 11.84 GGIPTDD380 pKa = 3.18 AGSRR384 pKa = 11.84 RR385 pKa = 11.84 GEE387 pKa = 4.04 WTHH390 pKa = 6.72 GPVV393 pKa = 3.45
Molecular weight: 43.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.299
IPC2_protein 6.211
IPC_protein 6.326
Toseland 6.59
ProMoST 6.737
Dawson 6.678
Bjellqvist 6.649
Wikipedia 6.693
Rodwell 6.678
Grimsley 6.722
Solomon 6.693
Lehninger 6.693
Nozaki 6.956
DTASelect 7.117
Thurlkill 7.146
EMBOSS 7.132
Sillero 7.073
Patrickios 4.495
IPC_peptide 6.707
IPC2_peptide 6.898
IPC2.peptide.svr19 6.827
Protein with the highest isoelectric point:
>tr|A0A5Q2W7X5|A0A5Q2W7X5_9VIRU Rep protein OS=CRESS virus sp. ctxk12 OX=2656692 PE=4 SV=1
MM1 pKa = 7.92 PLPFFTAAGVAAAGIASYY19 pKa = 10.77 LGIGTEE25 pKa = 4.13 GRR27 pKa = 11.84 EE28 pKa = 4.09 ALGKK32 pKa = 10.78 GLGQLSSAADD42 pKa = 3.62 LALDD46 pKa = 3.73 VAEE49 pKa = 5.57 LYY51 pKa = 10.26 PGAPWFSQGARR62 pKa = 11.84 YY63 pKa = 9.33 VRR65 pKa = 11.84 DD66 pKa = 3.95 YY67 pKa = 11.2 ISGVHH72 pKa = 5.26 GMHH75 pKa = 6.97 RR76 pKa = 11.84 KK77 pKa = 7.35 VQGMLGDD84 pKa = 3.87 RR85 pKa = 11.84 DD86 pKa = 3.79 VHH88 pKa = 5.75 PVQVPGYY95 pKa = 7.3 SEE97 pKa = 4.1 RR98 pKa = 11.84 VPYY101 pKa = 10.35 DD102 pKa = 3.34 SYY104 pKa = 12.07 KK105 pKa = 10.28 LDD107 pKa = 2.92 SWGIPRR113 pKa = 11.84 HH114 pKa = 3.54 QTYY117 pKa = 8.29 WVRR120 pKa = 11.84 EE121 pKa = 4.09 HH122 pKa = 7.17 PSFFRR127 pKa = 11.84 PYY129 pKa = 9.55 VGRR132 pKa = 11.84 RR133 pKa = 11.84 SQLSYY138 pKa = 11.21 NKK140 pKa = 8.72 TRR142 pKa = 11.84 APRR145 pKa = 11.84 VGYY148 pKa = 8.54 WRR150 pKa = 11.84 PPLSAPRR157 pKa = 11.84 RR158 pKa = 11.84 SIMPRR163 pKa = 11.84 RR164 pKa = 11.84 FRR166 pKa = 11.84 RR167 pKa = 11.84 RR168 pKa = 11.84 FTRR171 pKa = 11.84 PRR173 pKa = 11.84 FRR175 pKa = 11.84 RR176 pKa = 11.84 RR177 pKa = 11.84 FRR179 pKa = 11.84 RR180 pKa = 11.84 RR181 pKa = 11.84 YY182 pKa = 7.79 GGYY185 pKa = 8.99 RR186 pKa = 11.84 RR187 pKa = 11.84 RR188 pKa = 11.84 RR189 pKa = 11.84 SFGVKK194 pKa = 8.64 RR195 pKa = 11.84 RR196 pKa = 11.84 RR197 pKa = 11.84 FVYY200 pKa = 9.75 RR201 pKa = 11.84 RR202 pKa = 11.84 ARR204 pKa = 11.84 RR205 pKa = 11.84 LIASMRR211 pKa = 11.84 PKK213 pKa = 10.47 RR214 pKa = 11.84 ADD216 pKa = 3.09 VQVGHH221 pKa = 6.96 KK222 pKa = 10.16 SVAANALTNQASPTFGASGNVLYY245 pKa = 10.14 ISPMRR250 pKa = 11.84 MPARR254 pKa = 11.84 PGGQYY259 pKa = 10.68 DD260 pKa = 3.07 IGGTKK265 pKa = 10.34 YY266 pKa = 10.52 PLRR269 pKa = 11.84 TAANQMQGRR278 pKa = 11.84 KK279 pKa = 8.86 IRR281 pKa = 11.84 LHH283 pKa = 5.34 RR284 pKa = 11.84 LKK286 pKa = 10.79 FKK288 pKa = 11.22 FEE290 pKa = 3.66 FWYY293 pKa = 10.09 DD294 pKa = 3.39 QSVMYY299 pKa = 10.7 GLDD302 pKa = 3.55 YY303 pKa = 11.35 SGGAPTPAFSSFHH316 pKa = 4.51 VWFGLFRR323 pKa = 11.84 KK324 pKa = 10.27 NIGSVANAYY333 pKa = 9.96 AQFGLYY339 pKa = 9.85 SDD341 pKa = 4.93 SLNSSDD347 pKa = 4.66 KK348 pKa = 10.63 PYY350 pKa = 10.39 IFYY353 pKa = 10.7 RR354 pKa = 11.84 RR355 pKa = 11.84 NHH357 pKa = 6.34 DD358 pKa = 3.67 TLNTLGDD365 pKa = 3.9 FSVGQQWHH373 pKa = 4.37 QRR375 pKa = 11.84 EE376 pKa = 3.77 VRR378 pKa = 11.84 YY379 pKa = 8.95 WLRR382 pKa = 11.84 HH383 pKa = 4.57 PRR385 pKa = 11.84 FLKK388 pKa = 9.82 YY389 pKa = 10.36 FKK391 pKa = 11.02 LLGHH395 pKa = 5.53 KK396 pKa = 9.45 HH397 pKa = 4.88 YY398 pKa = 10.71 RR399 pKa = 11.84 VGPEE403 pKa = 3.4 QNIWARR409 pKa = 11.84 TSAGTNVYY417 pKa = 10.29 EE418 pKa = 5.15 PIRR421 pKa = 11.84 KK422 pKa = 9.08 DD423 pKa = 3.3 VAPVLGAAAGQFTFRR438 pKa = 11.84 SRR440 pKa = 11.84 KK441 pKa = 8.02 GLVMEE446 pKa = 5.28 RR447 pKa = 11.84 DD448 pKa = 3.44 QDD450 pKa = 4.29 SVGVTPAALVDD461 pKa = 3.96 GALPLYY467 pKa = 10.55 AGQCDD472 pKa = 3.71 AFQCVYY478 pKa = 10.67 GAVPFMVIYY487 pKa = 10.97 CEE489 pKa = 4.13 DD490 pKa = 3.75 TSGLTAEE497 pKa = 5.12 ANWTLNSTYY506 pKa = 10.51 AISWNEE512 pKa = 3.43
Molecular weight: 58.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.043
IPC_protein 10.906
Toseland 10.555
ProMoST 10.452
Dawson 10.76
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.818
Grimsley 10.847
Solomon 10.862
Lehninger 10.804
Nozaki 10.54
DTASelect 10.54
Thurlkill 10.613
EMBOSS 10.979
Sillero 10.687
Patrickios 10.35
IPC_peptide 10.847
IPC2_peptide 9.633
IPC2.peptide.svr19 8.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
905
393
512
452.5
51.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.84 ± 0.12
1.105 ± 0.431
5.746 ± 1.366
4.088 ± 1.287
4.309 ± 0.963
10.387 ± 0.841
2.762 ± 0.186
3.978 ± 0.871
4.309 ± 0.66
7.072 ± 0.196
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.099 ± 0.041
2.431 ± 0.414
5.746 ± 0.419
3.867 ± 0.195
10.276 ± 1.361
5.856 ± 0.814
4.972 ± 0.886
4.751 ± 1.57
2.762 ± 0.51
4.641 ± 1.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here