Mycoplasma crocodyli (strain ATCC 51981 / MP145)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma crocodyli

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 689 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5E5K4|D5E5K4_MYCCM 30S ribosomal protein S1 OS=Mycoplasma crocodyli (strain ATCC 51981 / MP145) OX=512564 GN=rpsA PE=4 SV=1
MM1 pKa = 7.67LIDD4 pKa = 3.81GNKK7 pKa = 9.19NKK9 pKa = 10.57SIIYY13 pKa = 8.51FVVDD17 pKa = 4.5GEE19 pKa = 4.93DD20 pKa = 2.98IDD22 pKa = 4.2YY23 pKa = 10.95QPSSEE28 pKa = 3.98VDD30 pKa = 3.69VVDD33 pKa = 3.93GFYY36 pKa = 11.06NLEE39 pKa = 3.95INSLNKK45 pKa = 10.27LKK47 pKa = 10.65NDD49 pKa = 3.4SYY51 pKa = 11.89VVFRR55 pKa = 11.84DD56 pKa = 3.66YY57 pKa = 11.43LSSFSANIKK66 pKa = 9.88ILEE69 pKa = 4.33ISLSDD74 pKa = 3.7YY75 pKa = 11.44SEE77 pKa = 4.02EE78 pKa = 4.15TLGNLIALIYY88 pKa = 9.43YY89 pKa = 9.47LNIYY93 pKa = 7.07YY94 pKa = 10.6ACLVGEE100 pKa = 4.41NPFINHH106 pKa = 6.71KK107 pKa = 10.41

Molecular weight: 12.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5E6G8|D5E6G8_MYCCM Putative esterase/lipase OS=Mycoplasma crocodyli (strain ATCC 51981 / MP145) OX=512564 GN=MCRO_0764 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.99RR4 pKa = 11.84TFQPNKK10 pKa = 8.36RR11 pKa = 11.84KK12 pKa = 8.93HH13 pKa = 5.88AKK15 pKa = 8.83VHH17 pKa = 5.34GFRR20 pKa = 11.84ARR22 pKa = 11.84MLTANGRR29 pKa = 11.84KK30 pKa = 9.04VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.11GRR40 pKa = 11.84KK41 pKa = 8.24QLTVSDD47 pKa = 4.08KK48 pKa = 11.41

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

689

0

689

254647

37

3422

369.6

42.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.01 ± 0.088

0.51 ± 0.024

5.649 ± 0.069

6.716 ± 0.096

5.299 ± 0.077

4.78 ± 0.1

1.42 ± 0.032

9.54 ± 0.103

10.668 ± 0.103

9.535 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.04

7.758 ± 0.109

2.637 ± 0.05

2.779 ± 0.038

2.769 ± 0.065

6.831 ± 0.069

5.437 ± 0.078

5.618 ± 0.065

0.886 ± 0.03

4.275 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski