Ceraceosorus bombacis
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8017 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P1BRA5|A0A0P1BRA5_9BASI Uncharacterized protein OS=Ceraceosorus bombacis OX=401625 PE=4 SV=1
MM1 pKa = 8.18 DD2 pKa = 4.64 SFINKK7 pKa = 9.65 AKK9 pKa = 10.75 DD10 pKa = 3.3 FAEE13 pKa = 4.16 SDD15 pKa = 3.39 QGKK18 pKa = 9.49 GLINKK23 pKa = 9.51 ALGNQGNQDD32 pKa = 3.42 NQNSYY37 pKa = 10.64 GGNNSSSNNDD47 pKa = 3.05 SYY49 pKa = 12.18 GSGNNSSSTGGFGSSNNDD67 pKa = 3.06 NNDD70 pKa = 3.15 SYY72 pKa = 12.2 GSGNKK77 pKa = 9.5 SSSGGGFGGSSDD89 pKa = 3.98 NYY91 pKa = 9.62 GSSGGRR97 pKa = 11.84 DD98 pKa = 3.39 NNDD101 pKa = 3.01 SYY103 pKa = 11.79 GSSGGRR109 pKa = 11.84 DD110 pKa = 3.39 NNDD113 pKa = 3.01 SYY115 pKa = 11.79 GSSGGRR121 pKa = 11.84 DD122 pKa = 3.35 NNNSYY127 pKa = 10.78 GSSGGRR133 pKa = 11.84 DD134 pKa = 3.39 NNDD137 pKa = 3.01 SYY139 pKa = 11.78 GSSGGRR145 pKa = 11.84 GNNDD149 pKa = 2.85 SYY151 pKa = 11.81 GSSGGNNDD159 pKa = 3.48 SYY161 pKa = 11.52 GSSGGRR167 pKa = 11.84 DD168 pKa = 3.39 NNDD171 pKa = 3.01 SYY173 pKa = 11.79 GSSGGRR179 pKa = 11.84 DD180 pKa = 3.18 NSDD183 pKa = 3.37 SYY185 pKa = 11.63 GSSGGRR191 pKa = 11.84 GNNDD195 pKa = 2.69 SFGGSGGNSGGFGSSGNDD213 pKa = 2.92 NSSYY217 pKa = 10.88 GGNSGTDD224 pKa = 3.29 SYY226 pKa = 11.96 GSGGRR231 pKa = 11.84 QGGGNSDD238 pKa = 3.84 SYY240 pKa = 12.03 GSGGNDD246 pKa = 2.73 NNYY249 pKa = 10.66
Molecular weight: 24.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.884
IPC_protein 3.923
Toseland 3.668
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.961
Rodwell 3.745
Grimsley 3.579
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.431
Thurlkill 3.757
EMBOSS 3.961
Sillero 4.062
Patrickios 1.163
IPC_peptide 3.935
IPC2_peptide 4.024
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A0P1BJ97|A0A0P1BJ97_9BASI Uncharacterized protein OS=Ceraceosorus bombacis OX=401625 PE=4 SV=1
MM1 pKa = 7.72 ASNARR6 pKa = 11.84 GGRR9 pKa = 11.84 GGGPGASQRR18 pKa = 11.84 LSATPTGNWRR28 pKa = 11.84 TGGPNGAASGASPAGGAGSRR48 pKa = 11.84 WAAGPPRR55 pKa = 11.84 NNQSAQNGNAXX66 pKa = 3.86
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8017
0
8017
3982424
49
5271
496.7
53.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.34 ± 0.03
1.018 ± 0.011
5.451 ± 0.016
5.87 ± 0.026
3.022 ± 0.016
7.319 ± 0.028
2.569 ± 0.015
3.778 ± 0.018
4.199 ± 0.025
8.952 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.835 ± 0.009
2.957 ± 0.012
6.12 ± 0.033
4.192 ± 0.016
6.97 ± 0.026
9.808 ± 0.046
5.582 ± 0.016
5.702 ± 0.018
1.261 ± 0.01
2.045 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here