Leifsonia xyli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3238 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172X6J5|A0A172X6J5_9MICO Uroporphyrinogen decarboxylase OS=Leifsonia xyli OX=1575 GN=hemE PE=3 SV=1
MM1 pKa = 7.65APTAPVDD8 pKa = 3.73EE9 pKa = 4.52VQRR12 pKa = 11.84AYY14 pKa = 10.4AAKK17 pKa = 8.87LTEE20 pKa = 4.92AGDD23 pKa = 3.61DD24 pKa = 3.52EE25 pKa = 4.43QRR27 pKa = 11.84RR28 pKa = 11.84DD29 pKa = 3.42TVTAARR35 pKa = 11.84DD36 pKa = 3.45ALLAASAWQAPSTPVAPPVAQAPTAPADD64 pKa = 4.1ASPQHH69 pKa = 6.78PGYY72 pKa = 10.81APPPSGAVPPPGYY85 pKa = 10.52GSGGPTVPPPPVYY98 pKa = 10.72APGGPTLPPQPPYY111 pKa = 10.2PAGSWYY117 pKa = 10.41AAPPPARR124 pKa = 11.84RR125 pKa = 11.84GLSTGAIVGITLGSIAVALVILIVVFVGIFAVAHH159 pKa = 5.48EE160 pKa = 4.49SSRR163 pKa = 11.84LAGSDD168 pKa = 3.43VGSSAAPYY176 pKa = 10.16DD177 pKa = 4.41DD178 pKa = 5.73SSDD181 pKa = 4.11DD182 pKa = 3.8PSQGGDD188 pKa = 3.1SASGQDD194 pKa = 3.45YY195 pKa = 11.06DD196 pKa = 3.57VDD198 pKa = 3.97GVHH201 pKa = 5.74VHH203 pKa = 6.38NVDD206 pKa = 2.55GWTFEE211 pKa = 4.0LTADD215 pKa = 3.89QTCSGAQITAGFSDD229 pKa = 4.63SPDD232 pKa = 4.04GDD234 pKa = 6.04DD235 pKa = 3.86LDD237 pKa = 3.6QWTTIVDD244 pKa = 4.21LEE246 pKa = 4.58AGVPFTLTIPDD257 pKa = 3.66SASDD261 pKa = 3.63YY262 pKa = 10.58DD263 pKa = 3.86YY264 pKa = 11.94VGIEE268 pKa = 4.48TVDD271 pKa = 3.45CTQAA275 pKa = 3.19

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172X6C5|A0A172X6C5_9MICO Uncharacterized protein OS=Leifsonia xyli OX=1575 GN=A0130_10685 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.07GRR40 pKa = 11.84TKK42 pKa = 10.91LSAA45 pKa = 3.61

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3238

0

3238

995190

27

2281

307.3

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.384 ± 0.061

0.469 ± 0.01

6.117 ± 0.039

5.494 ± 0.044

3.212 ± 0.027

8.967 ± 0.042

1.912 ± 0.023

4.438 ± 0.034

2.167 ± 0.034

10.172 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.676 ± 0.016

2.065 ± 0.024

5.482 ± 0.032

2.904 ± 0.026

7.179 ± 0.048

5.638 ± 0.036

6.147 ± 0.042

8.964 ± 0.041

1.52 ± 0.019

2.093 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski