Aestuariispira insulae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Kiloniellaceae; Aestuariispira

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4490 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9H5Q5|A0A3D9H5Q5_9PROT Choline monooxygenase OS=Aestuariispira insulae OX=1461337 GN=DFP90_11323 PE=4 SV=1
MM1 pKa = 7.62SSLNSALSAAVSGLFAQSTSISAISTNLSNVGTTAYY37 pKa = 9.85KK38 pKa = 9.45EE39 pKa = 4.05ASVSFSTLVSGSTGNNRR56 pKa = 11.84SSNFNGSGVYY66 pKa = 10.32AGVNQSISHH75 pKa = 7.23LGNVSDD81 pKa = 4.29TAISTNIALTGSGFLVVSDD100 pKa = 4.73DD101 pKa = 3.7AAGDD105 pKa = 3.46EE106 pKa = 5.18LYY108 pKa = 10.54FSRR111 pKa = 11.84VGSFYY116 pKa = 10.12PDD118 pKa = 3.46KK119 pKa = 10.81DD120 pKa = 3.78DD121 pKa = 5.83KK122 pKa = 11.4LVNLNGFYY130 pKa = 10.78LQGYY134 pKa = 6.07PTDD137 pKa = 3.72ASGNPTVGTGSTSTLEE153 pKa = 4.38TIDD156 pKa = 4.07LGSIGGNAAATEE168 pKa = 4.49NIDD171 pKa = 2.96IRR173 pKa = 11.84ANIPANAAIGDD184 pKa = 4.12SFNTDD189 pKa = 2.06IEE191 pKa = 4.53MFDD194 pKa = 3.6SLGTSHH200 pKa = 6.86YY201 pKa = 9.73VTATWTKK208 pKa = 8.89TAANTWDD215 pKa = 3.15ITYY218 pKa = 10.57ADD220 pKa = 4.45PVLSADD226 pKa = 3.54STTTTGTVTGSSTITFDD243 pKa = 3.23SDD245 pKa = 3.78GNFVSSTSNNLAINGWTTGASNSAITMNFAGLTQFTKK282 pKa = 10.77DD283 pKa = 3.42EE284 pKa = 4.02QTLDD288 pKa = 3.56IEE290 pKa = 4.33IDD292 pKa = 3.86KK293 pKa = 10.89INQDD297 pKa = 3.05GLEE300 pKa = 4.02YY301 pKa = 11.28GEE303 pKa = 4.94LKK305 pKa = 10.64AVSIGSDD312 pKa = 3.5GLVTATFEE320 pKa = 4.4NGTSYY325 pKa = 10.7PVYY328 pKa = 9.74QIPVATFSNPNGLALEE344 pKa = 4.43SGNVYY349 pKa = 10.6SATNDD354 pKa = 3.02SGTYY358 pKa = 8.41TLHH361 pKa = 7.72DD362 pKa = 5.16PGDD365 pKa = 4.08GGAGEE370 pKa = 4.72LKK372 pKa = 10.65GSALEE377 pKa = 4.31GSTVDD382 pKa = 4.55TADD385 pKa = 3.46QLTKK389 pKa = 10.94LIVAQQAYY397 pKa = 8.45SACAEE402 pKa = 4.23IVSTTDD408 pKa = 4.17DD409 pKa = 3.72MFDD412 pKa = 3.62TLISAARR419 pKa = 3.5

Molecular weight: 43.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9HK64|A0A3D9HK64_9PROT CelD/BcsL family acetyltransferase involved in cellulose biosynthesis OS=Aestuariispira insulae OX=1461337 GN=DFP90_105261 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 5.66FGTWVIYY9 pKa = 10.36LFACAALCFTPGPNSLLALSHH30 pKa = 5.9GASYY34 pKa = 10.86GFKK37 pKa = 9.62RR38 pKa = 11.84TIFTSAGGALGFAAVIAVCLAGLGALLAASATAFLVIKK76 pKa = 10.29LVGAAYY82 pKa = 9.62LIWLGIQSLRR92 pKa = 11.84RR93 pKa = 11.84PAFVLGADD101 pKa = 3.75VAAKK105 pKa = 9.52GLRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84SHH112 pKa = 6.65FAQGFLAAATNPKK125 pKa = 10.29GILFFVAFLPQFYY138 pKa = 10.72DD139 pKa = 3.44PARR142 pKa = 11.84PAFLQFLILAATFASIEE159 pKa = 4.13FFLEE163 pKa = 3.69LALAALAHH171 pKa = 7.15RR172 pKa = 11.84LRR174 pKa = 11.84PWLAKK179 pKa = 10.51ASVGKK184 pKa = 9.21WFKK187 pKa = 10.64RR188 pKa = 11.84VTGASFIGIGAGLATASGRR207 pKa = 3.73

Molecular weight: 21.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4490

0

4490

1443071

28

3550

321.4

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.236 ± 0.044

0.975 ± 0.013

6.237 ± 0.042

6.487 ± 0.034

3.885 ± 0.027

8.185 ± 0.045

2.176 ± 0.021

5.726 ± 0.024

4.171 ± 0.031

10.307 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.817 ± 0.019

3.241 ± 0.024

4.62 ± 0.027

3.633 ± 0.026

6.113 ± 0.037

5.654 ± 0.031

5.028 ± 0.032

6.749 ± 0.035

1.271 ± 0.015

2.489 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski