Paracoccus halophilus
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A099EVS8|A0A099EVS8_9RHOB Uncharacterized protein OS=Paracoccus halophilus OX=376733 GN=IT41_18345 PE=4 SV=1
MM1 pKa = 7.66 TDD3 pKa = 3.21 YY4 pKa = 8.29 FTHH7 pKa = 6.94 FSCLLDD13 pKa = 3.32 VGTPEE18 pKa = 4.06 NAASALDD25 pKa = 4.61 LYY27 pKa = 11.2 NALADD32 pKa = 4.43 DD33 pKa = 4.82 NAAEE37 pKa = 5.01 DD38 pKa = 4.25 PPSDD42 pKa = 3.7 GFLLSIQPEE51 pKa = 4.26 HH52 pKa = 6.99 GGTQLWMRR60 pKa = 11.84 DD61 pKa = 3.8 DD62 pKa = 3.59 VTGDD66 pKa = 3.66 PEE68 pKa = 4.69 HH69 pKa = 6.94 VIQFVKK75 pKa = 10.27 RR76 pKa = 11.84 CARR79 pKa = 11.84 EE80 pKa = 3.73 FGLTGLWGMQYY91 pKa = 11.5 ANTCSKK97 pKa = 10.44 PRR99 pKa = 11.84 VDD101 pKa = 3.8 GFGGGAHH108 pKa = 6.1 VLDD111 pKa = 4.82 LATGEE116 pKa = 4.42 TVDD119 pKa = 4.8 WIYY122 pKa = 11.18 TDD124 pKa = 3.52 GWLSTVLGGGNPYY137 pKa = 10.08 EE138 pKa = 4.12
Molecular weight: 14.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.024
IPC_protein 3.999
Toseland 3.795
ProMoST 4.126
Dawson 3.999
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.834
Grimsley 3.706
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.355
Thurlkill 3.846
EMBOSS 3.948
Sillero 4.126
Patrickios 1.036
IPC_peptide 3.986
IPC2_peptide 4.101
IPC2.peptide.svr19 4.025
Protein with the highest isoelectric point:
>tr|A0A099F0C1|A0A099F0C1_9RHOB DeoR faimly transcriptional regulator OS=Paracoccus halophilus OX=376733 GN=IT41_12430 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.0 GRR40 pKa = 11.84 KK41 pKa = 8.91 SLSAA45 pKa = 3.86
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3457
0
3457
1072070
36
2098
310.1
33.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.032 ± 0.062
0.829 ± 0.013
5.927 ± 0.035
5.739 ± 0.039
3.517 ± 0.029
8.973 ± 0.042
2.017 ± 0.02
5.33 ± 0.03
2.587 ± 0.03
10.276 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.785 ± 0.02
2.404 ± 0.026
5.379 ± 0.03
3.231 ± 0.026
7.699 ± 0.049
4.938 ± 0.026
4.992 ± 0.026
6.837 ± 0.032
1.426 ± 0.018
2.081 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here