Thaumarchaeota archaeon SCGC AC-337_F14
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 560 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A329CLD4|A0A329CLD4_9ARCH Uncharacterized protein OS=Thaumarchaeota archaeon SCGC AC-337_F14 OX=1465461 GN=BGW87_1152 PE=4 SV=1
MM1 pKa = 7.47 NSYY4 pKa = 10.87 IMLVPVTILILAGTGVVSQAFAATVITIEE33 pKa = 4.14 TDD35 pKa = 2.71 KK36 pKa = 11.11 DD37 pKa = 3.61 IYY39 pKa = 11.37 DD40 pKa = 3.79 HH41 pKa = 7.26 ASTIILTGNVDD52 pKa = 3.68 PVDD55 pKa = 3.9 LRR57 pKa = 11.84 GSEE60 pKa = 3.96 VTIMCKK66 pKa = 10.19 SPGGMGVCGIYY77 pKa = 9.99 QLSVNSDD84 pKa = 3.57 GNFSVSINTATFLMKK99 pKa = 10.18 KK100 pKa = 10.19 DD101 pKa = 3.84 GIYY104 pKa = 10.3 EE105 pKa = 4.01 FQANYY110 pKa = 10.59 SGLADD115 pKa = 3.36 VTVSVEE121 pKa = 3.77 LTNAIEE127 pKa = 4.11 TSEE130 pKa = 4.15 TGTAEE135 pKa = 3.75 TGTAVIGTAVTGTGEE150 pKa = 4.16 AGTDD154 pKa = 3.1 EE155 pKa = 4.19 VSFYY159 pKa = 11.4 KK160 pKa = 10.66 LAPGQIEE167 pKa = 4.06 YY168 pKa = 10.45 DD169 pKa = 3.6 ITCNASPAFFANADD183 pKa = 3.71 DD184 pKa = 4.41 DD185 pKa = 5.48 SIVILLDD192 pKa = 3.49 ATDD195 pKa = 5.21 DD196 pKa = 4.76 GILTITLHH204 pKa = 7.28 DD205 pKa = 4.14 EE206 pKa = 4.52 LIKK209 pKa = 10.98 PFDD212 pKa = 4.67 DD213 pKa = 3.66 GTFVVIVDD221 pKa = 3.83 NQVMQDD227 pKa = 3.15 FTQIGNTLTIPCLAGTEE244 pKa = 4.24 KK245 pKa = 10.5 IEE247 pKa = 4.42 IHH249 pKa = 6.32 GSWAIPEE256 pKa = 4.3 FGVIAAMILAVAIISIIVVTAKK278 pKa = 9.5 TRR280 pKa = 11.84 LSIVPRR286 pKa = 11.84 YY287 pKa = 9.45
Molecular weight: 30.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.897
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.732
EMBOSS 3.821
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|A0A329CX59|A0A329CX59_9ARCH Uncharacterized protein OS=Thaumarchaeota archaeon SCGC AC-337_F14 OX=1465461 GN=BGW87_10233 PE=4 SV=1
MM1 pKa = 7.19 ATSKK5 pKa = 10.86 AKK7 pKa = 10.44 RR8 pKa = 11.84 SAAAKK13 pKa = 9.55 KK14 pKa = 9.82 AARR17 pKa = 11.84 TRR19 pKa = 11.84 KK20 pKa = 9.74 RR21 pKa = 11.84 NAAAKK26 pKa = 7.03 NTKK29 pKa = 9.94 RR30 pKa = 11.84 SAASKK35 pKa = 9.79 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.71 SSGGAKK44 pKa = 9.67 RR45 pKa = 11.84 KK46 pKa = 9.35 APKK49 pKa = 10.05 RR50 pKa = 11.84 KK51 pKa = 9.13 APKK54 pKa = 9.96 RR55 pKa = 11.84 KK56 pKa = 7.59 ATKK59 pKa = 10.2 RR60 pKa = 11.84 KK61 pKa = 6.9 ATKK64 pKa = 10.12 RR65 pKa = 11.84 KK66 pKa = 8.78 APKK69 pKa = 9.87 RR70 pKa = 11.84 KK71 pKa = 9.21 APKK74 pKa = 9.6 RR75 pKa = 11.84 KK76 pKa = 8.99 AAAKK80 pKa = 9.59 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 3.55
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.481
IPC2_protein 11.52
IPC_protein 13.071
Toseland 13.247
ProMoST 13.729
Dawson 13.247
Bjellqvist 13.232
Wikipedia 13.715
Rodwell 13.115
Grimsley 13.29
Solomon 13.744
Lehninger 13.642
Nozaki 13.247
DTASelect 13.232
Thurlkill 13.247
EMBOSS 13.744
Sillero 13.247
Patrickios 12.822
IPC_peptide 13.744
IPC2_peptide 12.72
IPC2.peptide.svr19 9.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
560
0
560
139431
26
1264
249.0
28.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.738 ± 0.09
1.11 ± 0.039
5.898 ± 0.068
6.987 ± 0.094
4.277 ± 0.08
6.341 ± 0.096
1.87 ± 0.038
8.784 ± 0.096
9.286 ± 0.134
8.753 ± 0.086
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.454 ± 0.048
5.109 ± 0.079
3.667 ± 0.053
3.037 ± 0.054
3.924 ± 0.076
6.909 ± 0.088
5.389 ± 0.068
6.346 ± 0.079
0.928 ± 0.041
3.192 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here